Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes

Hart, Prue H.; Brand, Catherine; Carson, Christine; Riley, Thomas V.; Prager, R. H.; Finlay‐Jones, John J.

doi:10.1007/s000110050639

Cited by 335 publications

(255 citation statements)

References 16 publications

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“…In an in vitro study, concentrations of 300 mg/mL and 500 mg/mL of TTO caused 50% mortality of the human ephitelial cells and fi broblasts after 24 or 48 hours of incubation (Soderberg et al, 1996). In another study in vitro, the tea tree oil emulsifi ed into culture medium containing 10% fetal calf serum was toxic to monocytes at a concentration of 0.016% v/v (Hart et al, 2000) and for neutrophils in the same concentration (Brand et al, 2001). The high concentration (20%) of neutral gel used in this experiment could be the reason for the toxicity of the cells.…”

Section: Discussionmentioning

confidence: 99%

“…It was demonstrated that terpeno-4-ol, the largest component of essential oil of Melaleuca, suppresses the synthesis of infl ammatory mediators for activated human monocytes. The composition inhibited the production of cytokines such as Tumor Necrosis Factor (TNF), Interleukin-1b (IL-1b), IL-8 and IL-10, as well as the lipidic mediator prostaglandin, when the monocytes are activated by bacterial lipopolysaccharide (Hart et al, 2000). This essential oil is a compound of approximately 100 components.…”

Section: Discussionmentioning

confidence: 99%

“…Australian natives believe that oil of Melaleuca has antiseptic activities (Schulz, 2000). Oil of Melaleuca or Tea Tree Oil (TTO) has been reported to have anti-bacterial (Carson et al, 2006;Luz;Janaina, 2007), antifungic (Hammer et al, 1997, Oliva et al, 2003, antiviral (Bishop, 1995), anti-tumor activity (Calcabrini et al, 2004) and anti-infl ammatory properties in vitro (Brand et al, 2001;Hart et al, 2000). Oil of Melaleuca has a wide spectrum of activity that is mainly attributed to terpeno-4-ol.…”

Section: Introductionmentioning

confidence: 99%

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The influence of the essential oil of Melaleuca alternifolia on the healing of infected dental alveoli: a histological study in rats

Kreuger¹,

Ternes²,

Mello³

et al. 2007

Rev. bras. farmacogn.

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RESUMO: "Infl uência do óleo essencial de Melaleuca alternifolia na cicatrização de alveolite dental infectada: um estudo histológico em ratos". A planta Melaleuca alternifolia é nativa da Austrália e a destilação de suas folhas produz um óleo essencial conhecido por óleo de Melaleuca, ou Tea tree oil, usado como antimicrobiano. Nesse estudo, foi verifi cado a atividade deste óleo no processo de reparo de alvéolos dentais infectados. 48 ratos foram utilizados (Rattus novergicus albinus, Wistar) e após a extração do dente e posterior infecção do alvéolo com Staphylococcus aureus, os animais foram separados em três grupos: Grupo I: curetagem e irrigação do alvéolo com soro fi siológico; Grupo II: curetagem e irrigação com soro fi siológico e tratamento com Rifocina 25 mg; e Grupo III: curetagem e irrigação com soro fi siológico e aplicação tópica de óleo de Melaleuca 20%. Os animais foram sacrifi cados 24 horas, 7, 14 e 21 dias após o tratamento e o processo de reparo do alvéolo dental foi analisado por microscopia ótica. Os resultados foram submetidos à análise quantitativa e qualitativa e foi possível concluir que o óleo a 20% causou um retardo no processo de reparo dos alvéolos dentais infectados dos ratos, demonstrado por maior área de necrose e menor osteogênese. Unitermos: Melaleuca alternifolia, Staphylococcus aureus, alvéolo dental. ABSTRACT:The plant Melaleuca alternifolia is native to Australia. The distillation of its leaves produces an essential oil, commonly known as oil of Melaleuca, or Tea tree oil, which present antimicrobial activity. This study investigates the action of this oil on the repair process of infected dental alveoli. 48 rats were used (Rattus novergicus albinus, Wistar). After tooth extraction and posterior infection of the dental alveoli with Staphylococcus aureus, the animals were separated into three groups: Group I: curettage and irrigation with physiologic saline solution; Group II: curettage and irrigation with physiologic saline solution and topical application of rifamycin diethylamide B 25 mg; and Group III: curettage and irrigation with physiologic saline solution and topical application of oil of Melaleuca 20%. The animals were sacrifi ced 24 hours, 7, 14 and 21 days after the treatment with powder and the repair process of the dental alveoli was analyzed using an optical microscope. The results were submitted to qualitative and quantitative analysis and it was concluded that tea tree oil at 20% caused a delay in the repair process of infected dental alveoli in rats, as demonstrated by the presence of more necrosis area and less osteogenesis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The influence of the essential oil of Melaleuca alternifolia on the healing of infected dental alveoli: a histological study in rats

Kreuger¹,

Ternes²,

Mello³

et al. 2007

Rev. bras. farmacogn.

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“…(Paisooksantivatana and Buain, 2009), leaves and terminal branches of Melaleuca alternifolia (Myrtales: Myrtaceae) (Pazyar et al, 2013). Terpinen-4-ol has many pharmacological activities such as anti-inflammation (Hart et al, 2000;Jeenapongsa et al, 2003), and anti-hypersensitivity (Lahlou et al, 2003). Furthermore, bactericidal (Loughlin et al, 2008) and antifungal properties (Mondello et al, 2006) have also been found in terpinen-4-ol.…”

Section: Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death Inmentioning

confidence: 99%

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Banjerdpongchai¹,

Khaw-on²

2013

Asian Pacific Journal of Cancer Prevention

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“…(Bua-in and Paisooksantivatana, 2009). It is used as herbal medicine with several activities, such as antiinflammation (Hart et al, 2000), anti-hypersensitivity …”

Section: Introductionmentioning

confidence: 99%

Induction of Intrinsic and Extrinsic Apoptosis Pathways in the Human Leukemic MOLT-4 Cell Line by Terpinen-4-ol

Khaw-on¹,

Banjerdpongchai²

2012

Asian Pacific Journal of Cancer Prevention

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Terpinen-4-ol is a terpene found in the rhizome of Plai (Zingiber montanum (Koenig) Link ex Dietr.). In this study apoptogenic activity and mechanisms of cell death induced by terpinen-4-ol were investigated in the human leukemic MOLT-4 cell line. Terpinen-4-ol exhibited cytotoxicity in MOLT-4 cells, with characteristic morphological features of apoptosis by Wright's staining. The mode of cell death was confirmed to be apoptosis by flow cytometric analysis after staining with annexin V-FITC and propidium iodide. A sub-G1 peak in DNA histograms of cell cycle assays was observed. Terpinen-4-ol induced-MOLT-4 cell apoptosis mediated through an intrinsic pathway involving the loss of mitochondrial transmembrane potential (MTP) and release of cytochrome c into the cytosol. In addition, terpinen-4-ol also induced apoptosis via an extrinsic pathway by caspase-8 activation resulting in the cleavage of cytosolic Bid. Truncated-Bid (tBid) translocated to mitochondria and activated the mitochondrial pathway in conjunction with down-regulation of Bcl-2 protein expression. Caspase-3 activity also increased. In conclusion, terpinen-4-ol can induce human leukemic MOLT-4 cell apoptosis via both intrinsic and extrinsic pathways.

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Terpinen-4-ol, the main component of the essential oil of Melaleuca alternifolia (tea tree oil), suppresses inflammatory mediator production by activated human monocytes

Abstract: The water-soluble components of tea tree oil can suppress pro-inflammatory mediator production by activated human monocytes.

Cited by 335 publications

References 16 publications

The influence of the essential oil of Melaleuca alternifolia on the healing of infected dental alveoli: a histological study in rats

The influence of the essential oil of Melaleuca alternifolia on the healing of infected dental alveoli: a histological study in rats

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Induction of Intrinsic and Extrinsic Apoptosis Pathways in the Human Leukemic MOLT-4 Cell Line by Terpinen-4-ol

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