Induction of Intrinsic and Extrinsic Apoptosis Pathways in the Human Leukemic MOLT-4 Cell Line by Terpinen-4-ol

Khaw-on, Patompong; Banjerdpongchai, Ratana

doi:10.7314/apjcp.2012.13.7.3073

Cited by 24 publications

(18 citation statements)

References 13 publications

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“…Percent cell viability of HL-60 and MOLT-4 cells were reduced by terpinen-4-ol with a dose-dependence manner, whereas terpinen-4-ol had no cytotoxic effect on U937 as shown in Figure 1 and our previous report (Khaw-on and Banjerdpongchai, 2012). The IC 20 and IC 50 values (inhibitory concentration at 20% and 50%) of the terpinen-4-ol on HL-60 cells were 12 and 30 µM, respectively, which were used for further experiments.…”

Section: Cytotoxic Effect Of Terpinen-4-ol On Human Leukemic Hl-60 Anmentioning

confidence: 70%

“…Apoptosis induced by terpinen-4-ol and its underlying mechanism have been previously studied, for example, terpinen-4-ol induces apoptosis in human non-small cell lung cancer (NSCLC) cells (Wu et al, 2012). Terpinen-4-ol is able to induce apoptosis in human leukemia MOLT-4 cells through both the mitochondriamediated intrinsic pathway indicating by the cytochrome c release and the loss of mitochondrial transmembrane potential (MTP) (Khaw-on and Banjerdpongchai, 2012). Moreover, it has revealed that terpinen-4-ol induces apoptosis in human leukemic MOLT-4 cells in vitro via caspase-8-activated extrinsic pathways and also cross talked to intrinsic pathway by the presence of truncated Bid protein.…”

Section: Discussionmentioning

confidence: 99%

“…The results of MTT assay demonstrated that terpinen-4-ol treatment significantly induced cell death in human leukemic HL-60 cells but not in U937 cells. The MTT assay indicated that HL-60 cells exhibited the greatest sensitivity to terpinen-4-ol-induced cytotoxicity with the lower level of IC 50 (30 µM) compared to MOLT-4 cells (155 µM, in our previous study) ( Khaw-on and Banjerdpongchai, 2012).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Banjerdpongchai¹,

Khaw-on²

2013

Asian Pacific Journal of Cancer Prevention

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Section: Cytotoxic Effect Of Terpinen-4-ol On Human Leukemic Hl-60 Anmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Banjerdpongchai¹,

Khaw-on²

2013

Asian Pacific Journal of Cancer Prevention

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“…Moreover, the use of sesame oil did not need the hexane or methanol elimination process. For example, sabinene and terpinen-4-ol in Zingiber cassumunar 14,15 and zerumbone in Zingiber zerumbet 16 are water insoluble, so sesame oil can be employed to extract these substances from herbs. Moreover, the combination of sesame oil and herbal ingredients might provide benefits in terms of preserving the quality of active ingredients and preventing the lipid oxidation.…”

Section: Introductionmentioning

confidence: 99%

“…: This species has been widely researched and it was shown that terpinen-4-ol was the active compound which caused leukemic cells apoptosis. 14 It also has anti-inflammatory and anti-cancer properties. 17-21 …”

Section: Introductionmentioning

confidence: 99%

Properties of macerated herbal oil

Kantawong¹,

Singhatong²,

Srilamay³

et al. 2017

Bioimpacts

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Introduction: The addition of herbs into hot sesame oil could increase the oil-pulling efficiency of sesame oil. The aim of present study was to modify the proportion of herbs and sesame oil with the addition of other ingredients including menthol, camphor, and borneol and improve the medicinal properties and the scent of the oil. Methods: Macerated herbal oil was prepared by heat extraction of five species of herbs (Zingiber cassumunar, Zingiber zerumbet, Plantago major Linn, Citrus hystrix, and Amomum biflorum) with hot sesame oil. The study was performed to evaluate the anti-oxidant, anti-inflammatory, and anti-bacterial properties of this macerated herbal oil. Results: Macerated herbal oil was evaluated for antioxidant activity using DPPH and ABTS assays. It was shown that at dilution 1:2 in DMSO, the macerated herbal oil had DPPH and ABTS radical scavenging activities equal to 63% and 22%, respectively. Macerated herbal oil dilution 1:8 in DMSO demonstrated ferric reducing capacity equivalent to ascorbic acid (0.208 µM) and had reducing power equivalent to butylated hydroxytoluene (BHT) 7.41 µg/mL. MTT assay was performed using immortalized human mesenchymal stem cells (HMSCs) as a cell culture model. The result indicated that the cytotoxic concentration of the macerated herbal oil was ≥ 2.5 µL/mL in complete DMEM. Anti-inflammatory effects were evaluated using the nitrite assay and RT-PCR. It was found that the macerated herbal oil could inhibit nitrite accumulation in culture media. Change in the expression of COX-2, Nrf2, and NF-kB in RT-PCR confirmed the anti-inflammatory activity of the macerated herbal oil. Conclusion: It could be concluded that the macerated herbal oil could inhibit nitrite accumulation in culture media, which might be the inhibitory effect of the macerated herbal oil on COX-2 or Nrf2, the downstream modulator of the COX-2 pathway. Further intensive studies are needed for the optimization before bringing this macerated herbal oil into clinical application.

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Mono(2-ethylhexyl) phthalate induces apoptosis in p53-silenced L02 cells via activation of both mitochondrial and death receptor pathways

et al. 2014

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Mono(2-ethylhexyl) phthalate (MEHP) is one of the main metabolites of di(2-ethylhexyl) phthalate. The evidence shows that DEHP may exert its toxic effects primarily via MEHP, which is 10-fold more potent than its parent compound in toxicity in vitro. MEHP-induced apoptosis is mediated by either p53-dependent or -independent pathway. However, the detailed mechanism of its toxicity remains unclear. In this study, immortalized normal human liver cell line L02 was chosen, as an in vitro model of nonmalignant liver, to elucidate the role of p53 in MEHP-induced apoptosis. The cells were treated with MEHP (6.25, 12.50, 25.00, 50.00, and 100.00 μM) for 24 and 36 h, then small interfering RNA (siRNA) was used to specifically silence p53 gene of L02 cells. The results indicated that MEHP caused oxidative DNA damage and apoptosis in L02 cells were associated with the p53 signaling pathway. Further study found that MEHP (50.00 and 100.00 μM) induced apoptosis in p53-silenced L02 cells, along with the up-regulations of Fas and FasL proteins as well as increased the Bax/Bcl-2 ratio and Caspase 3, 8, and 9 activities. Additionally, both FasL inhibitor (AF-016) and Caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp- fluoromethylketone (Z-VAD-FMK) could prevent the cell apoptosis induced by MEHP. The findings suggested that MEHP-induced apoptosis in L02 cells involving a Caspases-mediated mitochondrial signaling pathway and/or death receptor pathway. p53 was not absolutely necessary for MEHP-induced L02 cell apoptosis.

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Induction of Intrinsic and Extrinsic Apoptosis Pathways in the Human Leukemic MOLT-4 Cell Line by Terpinen-4-ol

Cited by 24 publications

References 13 publications

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Terpinen-4-ol Induces Autophagic and Apoptotic Cell Death in Human Leukemic HL-60 Cells

Properties of macerated herbal oil

Mono(2-ethylhexyl) phthalate induces apoptosis in p53-silenced L02 cells via activation of both mitochondrial and death receptor pathways

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