Terpenoids with antifungal activity trigger mitochondrial dysfunction in Saccharomyces cerevisiae

Haque, Ejazul; Irfan, Safia; Kamil, Mohd; Sheikh, Saba; Hasan, Adria; Ahmad, Aijaz; Lakshmi, Vijai; Nazir, Aamir; Mir, Snober S.

doi:10.1134/s0026261716040093

Cited by 49 publications

(24 citation statements)

References 29 publications

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“…In another situation, EOs of Origanum compactum , Artemisia herba alba and Cinnamomum camphora showed an increase of the number of cytoplasmic petite mutations, i.e., mitochondrial damage, in Saccharomyces cerevisiae [ 34 ]. Haque et al [ 35 ] indicated that terpenoids could play a key role in diminishing the mitochondrial content, which gives rise to an altered level of reactive oxygen species (ROS) and ATP generation.…”

Section: Antifungal Activitymentioning

confidence: 99%

“…The fungal plasma membrane H + -ATPase plays an important role in the physiology of the fungal cell by supporting the large transmembrane electrochemical proton gradient across cell membrane necessary for nutrient uptake. The H + -ATPase also regulates intracellular pH and fungal cell growth; furthermore, it is involved in fungal pathogenicity through its effects on dimorphism, nutrient uptake, and medium acidification [ 35 , 36 , 37 ]. Inhibition of H + -ATPase leads to intracellular acidification and cell death.…”

Section: Antifungal Activitymentioning

confidence: 99%

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Essential Oils and Antifungal Activity

et al. 2017

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Since ancient times, folk medicine and agro-food science have benefitted from the use of plant derivatives, such as essential oils, to combat different diseases, as well as to preserve food. In Nature, essential oils play a fundamental role in protecting the plant from biotic and abiotic attacks to which it may be subjected. Many researchers have analyzed in detail the modes of action of essential oils and most of their components. The purpose of this brief review is to describe the properties of essential oils, principally as antifungal agents, and their role in blocking cell communication mechanisms, fungal biofilm formation, and mycotoxin production.

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Section: Antifungal Activitymentioning

confidence: 99%

Section: Antifungal Activitymentioning

confidence: 99%

Essential Oils and Antifungal Activity

et al. 2017

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“…In addition to these pharmacological activities, antimicrobial properties have also been demonstrated for these and other related triterpenoids such as tingenone, netzahualcoyone, or zeylasterone that exhibit inhibitory activity against Gram-positive bacteria [ 14 , 15 , 16 , 17 ]. Moreover, the anti-biofilm [ 18 , 19 , 20 ] and anti-fouling properties of celastrol and pristimerin have been reported on a wide variety of microorganisms [ 21 ], as well as anti-mycotic activity [ 22 , 23 , 24 , 25 , 26 , 27 ]. Besides, celastrol and its derivatives have also shown inhibitory activity against viruses producing hepatitis B and C [ 28 , 29 ], which makes them potentially useful compounds for the control of various diseases.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Mode of Action of Celastrol, a Nortriterpen Quinone Isolated from Natural Sources

Padilla-Montaño

Guerra

Moujir

2021

Foods

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Species of the Celastraceae family are traditionally consumed in different world regions for their stimulating properties. Celastrol, a triterpene methylene quinone isolated from plants of celastraceas, specifically activates satiety centers in the brain that play an important role in controlling body weight. In this work, the antimicrobial activity and mechanism of action of celastrol and a natural derivative, pristimerin, were investigated in Bacillus subtilis. Celastrol showed a higher antimicrobial activity compared with pristimerin, being active against Gram-positive bacteria with minimum inhibitory concentrations (MICs) that ranged between 0.16 and 2.5 µg/mL. Killing curves displayed a bactericidal effect that was dependent on the inoculum size. Monitoring of macromolecular synthesis in bacterial populations treated with these compounds revealed inhibition in the incorporation of all radiolabeled precursors, but not simultaneously. Celastrol at 3 µg/mL and pristimerin at 10 µg/mL affected DNA and RNA synthesis first, followed by protein synthesis, although the inhibitory action on the uptake of radiolabeled precursors was more dramatic with celastrol. This compound also caused cytoplasmic membrane disruption observed by potassium leakage and formation of mesosome-like structures. The inhibition of oxygen consumption of whole and disrupted cells after treatments with both quinones indicates damage in the cellular structure, suggesting the cytoplasmic membrane as a potential target. These findings indicate that celastrol could be considered as an interesting alternative to control outbreaks caused by spore-forming bacteria.

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“…It was classified in the diterpene group. Haque and co-workers [38] explained that terpenoids from the plant extract reduce the mitochondrial content of fungus, which could alter the ATP generation and level of reactive oxygen species. Consequently, the mitochondria of the fungus become dysfunctional.…”

Section: Discussionmentioning

confidence: 99%

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

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Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent.

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Terpenoids with antifungal activity trigger mitochondrial dysfunction in Saccharomyces cerevisiae

Cited by 49 publications

References 29 publications

Essential Oils and Antifungal Activity

Essential Oils and Antifungal Activity

Antimicrobial Activity and Mode of Action of Celastrol, a Nortriterpen Quinone Isolated from Natural Sources

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

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