Terpenoid phytoalexins

Brooks, C. J. W.; Watson, D. G.

doi:10.1039/np9910800367

Cited by 35 publications

(19 citation statements)

References 23 publications

(32 reference statements)

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“…In cell and organ cultures, biotic and abiotic elicitors have effectively stimulated production of almost all chemical classes of plant secondary metabolites (Brooks and Watson, 1991), such as coumarin derivatives (Conrath et al, 1989), thiophenes (Mukundan and Hjortso, 1990), alkaloids (Tyler et ah, 1989), terpenoids (Bostock et al, 1982), and flavonoids (Franceschi and Grimes, 1991;Tamari et al, 1995). In production of sanguinarine from cell suspension cultures of Papaver snmniverum, an elicitor was successfully used on an industrial scale (Park et al, 1990).…”

Section: Introductionmentioning

confidence: 98%

Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium phalae)

Fang

Smith

Pépin

1999

In Vitro Cell.Dev.Biol.-Plant

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Elicitation of anthocyanin-producing cells of ohelo (Vaccinium pahalae) by both biotic (purified 13-glucan and chitosan) and abiotic [sodium ferric ethylenediamine di-(o-hydroxyphenylacetate) FeEDDHA, and CuS04] elicitors resulted in significant enhancement of anthocyanin accumulation. Anthocyanin production increased up to 1.8 and 1.5-fold over the control in the presence of abiotic elicitors (90 I.tM FeEDDHA and 20 ~M CuSO a, respectively), and increased 1.9 and 1.6-fold in the presence of biotic elicitors (10 mg L 1 ]3-glucan and 100 mg L -1 chitosan). Maximum anthocyanin production with the two most effective elicitors was achieved when cultures were treated on Day 3 ([3-glucan) or Day 0 (FeEDDHA) after the initiation of fresh cell cultures. A concentration-dependent response was exhibited by cultures treated with exogenous methyl jasmonate (M J). The addition of 0.5 p,M MJ alone provoked a 2-3-fold increase in anthocyanin production over that of the control; however, no additive effect on anthocyanin production was observed in any treatments which combined MJ and [3-glucan or FeEDDHA. Conditioning of the cells with a preculture in either M J, ]3-glucan, or FeEDDHA similarly did not enhance anthocyanin production. Inoculation of cultures elicited by MJ or 6-glucan with ibuprofen, a reported inhibitor of jasmonate biosynthesis, dramatically stimulated, rather than inhibited, anthocyanin production, resuiting in levels of accumulation beyond any of the tested elicitor combinations. Hypotheses for the observed influence of ibuprofen in this system are discussed.

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Section: Introductionmentioning

confidence: 98%

Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium phalae)

Fang

Smith

Pépin

1999

In Vitro Cell.Dev.Biol.-Plant

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“…By considering that the physiologically active epimer of jasmonate [(+)-epijasmonate] represents only 5% of the total jasmonate in natural and synthetic samples (24,25) due to rapid epimerization and that exogenously applied jasmonate is rapidly metabolized in plants (26), the physiological concentration measured after elicitation ( Fig. 1) and the optimal exogenously applied concentration of the correct stereoisomer were both in the range of [1][2][3][4][5][6][7][8][9][10] gM. The HPLC profile of the alkaloid fraction of control and jasmonate-elicited cultures showed a general induction of alkaloid accumulation in the E. californica culture (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Fungal cell walls and fragments thereof (biotic elicitors) (3) trigger this defense response even in plant cell suspension cultures (4). The elicitor molecule interacts with a plant membrane receptor (5) that through an unknown mechanism activates specific genes (6), resulting in the synthesis of almost all chemical classes of secondary plant products (7). Jasmonic acid and its methyl ester have been 0 For jasmonic acid, R is H; for methyl jasmonate, R is CH3.…”

Section: Introductionmentioning

confidence: 99%

Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures.

Gundlach¹,

Müller²,

Kutchan³

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

1,013

562

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To deter pathogenic microorganisms and herbivores, plants have developed an inducible chemical defense system. It is known that the induced synthesis of low molecular weight compounds can be provoked by exposing cultured cells to fungal cell wall fragments. In this study we show that endogenous jasmonic acid and its methyl ester accumulate rapidly and transiently after treatment of plant cell suspension cultures of RauvolJ' canescens and Eschscholtia caisfornica with a yeast elicitor. Thirty-six plant species tested in cell suspension culture could be elicited with respect to the accumulation of secondary metabolites by exogenously supplied methyl jasmonate. Addition of methyl jasmonate initiates de novo transcription of genes, such as phenylalanine ammonia lyase, that are known to be involved in the chemical defense mechanisms of plants. These data demonstrate the integral role of jasmonic acid and its derivatives in the intracellular signal cascade that begins with interaction of an elicitor molecule with the plant cell surface and results, ultimately, in the accumulation of secondary compounds.

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“…It is thought that an elicitor molecule binds to a plant membrane receptor (Cosio et al 1992;Shibuya et al 1993) and activates the expression of a series of specific genes, resulting in the synthesis of phytoalexins (Brooks and Watson 1991). Biotic elicitors derived from the cell surface of pathogens and/ or host plants trigger the defense response.…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells

Sobajima

Takeda

Sugimori

et al. 2003

Planta

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In suspension-cultured rice (Oryza sativa L.) cells, jasmonic acid (JA) functions as a signal transducer in elicitor N-acetylchitoheptaose-induced phytoalexin production. Differential screening of a cDNA library constructed using poly(A) + RNA from suspension-cultured rice cells treated with JA (10 -4 M) for 2 h yielded a cDNA for a gene that responded to exogenous JA by an increase in mRNA level. Nucleotide sequence analysis indicated that the cDNA encodes an homologue of the yeast Old Yellow Enzyme. The deduced amino acid sequence was very similar to the sequences of 12-oxophytodienoic acid reductases (OPR) 1 and 2 from Arabidopsis thaliana (AtOPR1 and AtOPR2) and OPR1 from tomato (Lycopersicon esculentum) (LeOPR1). The cDNA-encoded protein purified from recombinant Escherichia coli cells as a hexahistidine-tagged fusion protein exhibited OPR activity similar to that of AtOPR1, AtOPR2, and LeOPR1, which catalyze reduction of (-)-cis-12-oxophytodienoic acid (OPDA) preferentially over (+)-cis-OPDA, a natural precursor of JA. Thus the rice enzyme was termed OsOPR1. The physiological roles of OsOPR1 are discussed. This is the first report of the cloning of an OPR gene from a monocot plant.

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Terpenoid phytoalexins

Cited by 35 publications

References 23 publications

Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium phalae)

Effects of exogenous methyl jasmonate in elicited anthocyanin-producing cell cultures of ohelo (Vaccinium phalae)

Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures.

Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells

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