Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells

Abstract

In suspension-cultured rice (Oryza sativa L.) cells, jasmonic acid (JA) functions as a signal transducer in elicitor N-acetylchitoheptaose-induced phytoalexin production. Differential screening of a cDNA library constructed using poly(A) + RNA from suspension-cultured rice cells treated with JA (10 -4 M) for 2 h yielded a cDNA for a gene that responded to exogenous JA by an increase in mRNA level. Nucleotide sequence analysis indicated that the cDNA encodes an homologue of the yeast Old Yellow Enzyme. The deduced amino acid sequence was very similar to the sequences of 12-oxophytodienoic acid reductases (OPR) 1 and 2 from Arabidopsis thaliana (AtOPR1 and AtOPR2) and OPR1 from tomato (Lycopersicon esculentum) (LeOPR1). The cDNA-encoded protein purified from recombinant Escherichia coli cells as a hexahistidine-tagged fusion protein exhibited OPR activity similar to that of AtOPR1, AtOPR2, and LeOPR1, which catalyze reduction of (-)-cis-12-oxophytodienoic acid (OPDA) preferentially over (+)-cis-OPDA, a natural precursor of JA. Thus the rice enzyme was termed OsOPR1. The physiological roles of OsOPR1 are discussed. This is the first report of the cloning of an OPR gene from a monocot plant.

GER1, a GDSL Motif‐Encoding Gene from Rice is a Novel Early Light‐ and Jasmonate‐Induced Gene

“…In summary, these findings show that GER1 is under control of both R light and the plant hormone JA. The same external stimuli also regulate the OPDA reductase of rice (OsOPR1), a JA-responsive gene in rice (Sobajima et al, 2003;Riemann et al, 2003). These findings are consistent with the abundance of light-, JA-, and pathogenesis-related motifs in the GER1 promoter (see supplemental data).…”

Safeners coordinately induce the expression of multiple proteins and MRP transcripts involved in herbicide metabolism and detoxification in Triticum tauschii seedling tissues

“…Besides acting as Phase I-related enzymes (mentioned previously), these OPRs may directly participate in the octadecanoid signaling pathway for JA biosynthesis, a signaling molecule that triggers plant defense responses against biotic and abiotic stresses [54]. In rice, diverse environmental stimuli as well as phytohormones (such as JA, salicylic acid, and ethylene) transiently regulate OsOPR1 transcripts [55] and the enzyme catalyzes the reduction of both cis-(2) -OPDA and cis-(1) -OPDA, with less activity toward cis-(1) -OPDA [56]. Three safener-inducible partial OPR cDNAs from T. tauschii seedlings have been cloned and sequenced in our laboratory (Zhang and Riechers, unpublished results), which have highest homology with rice OsOPR1 (ranging from 65 to 83% amino acid identity).…”

Comparative characterization and expression analysis of the four Old Yellow Enzyme homologues from Shewanella oneidensis indicate differences in physiological function

“…Some members of the OYE family are related quite distantly to OYE, including the oestrogen-binding protein of Candida albicans [3], the bile-acid-inducible flavoenzymes BaiH and BaiC [4] and trimethylamine dehydrogenase [5]. More closely related enzymes have been characterized in other yeasts, Grampositive and Gram-negative bacteria, monocotyledonous and dicotyledonous plants, Caenorhabditis elegans and Trypanosoma cruzi [6][7][8][9][10][11][12][13][14][15][16]. Bacterial homologues that have been characterized so far include PETN (pentaerythritol tetranitrate) reductase [17], GTN (glycerol trinitrate) reductase [18], MR (morphinone reductase) [19], 2-cyclohexenone reductase [20], the xenobiotic reductases A and B from Pseudomonas sp.…”