GER1, a GDSL Motif‐Encoding Gene from Rice is a Novel Early Light‐ and Jasmonate‐Induced Gene

Abstract

The reaction of the rice mutant HEBIBA differs from that of wild-type rice in that the mutant responds inversely to red light and is defective in the light-triggered biosynthesis of jasmonic acid (JA). Using the wild type and the HEBIBA mutant of rice in a differential display screen, we attempted to identify genes that act in or near the convergence point of light and JA signalling. We isolated specifically regulated DNA fragments from approximately 10 000 displayed bands, and identified a new early light- and JA-induced gene. This gene encodes an enzyme containing a GDSL motif, showing 38 % identity at the amino acid level to lipase Arab-1 in Arabidopsis thaliana. The GDSL CONTAINING ENZYME RICE 1 gene (GER1) is rapidly induced by both red (R) and far-red (FR) light and by JA. The results are discussed with respect to a possible role for GER1 as a negative regulator of coleoptile elongation in the context of recent findings on the impact of JA on light signalling.

Multifunctionality and diversity of GDSL esterase/lipase gene family in rice (Oryza sativa L. japonica) genome: new insights from bioinformatics analysis

“…Subclade Ib ( OsGELP2 3 63 77 84 , and 85 ) is expected to be involved in plant development and morphogenesis at the seedling stage according to the function of their close homologue GER1 ( OsGELP33 ) [56]. These genes are not only expressed in many rice organs and development stages, as well as share analogous gene structure and special protein motif 32, but also change their expression dramatically under stress conditions during early plant development (Figures.…”

“…The OsGELP63 gene is induced by both red and far-red light and by jasmonic acid, and acts in response to drought and cold stresses [79]. The study of OsGELP33 ( GER1 ) has demonstrated the role of this gene in the rice plant development at the seedling and coleoptile elongation stages [56]. OsGELP33 , together with its sister genes OsGELP84 and OsGELP3 , arose from the segmental duplication event (Figure 2).…”

“…Currently, only two rice GDSL esterases/lipases genes have been reported. GDSL-containing enzyme rice 1 ( GER1 ) and wilted dwarf and lethal 1 ( WDL1 ) were cloned from the rice genome, and their physiologic functions were suggested as regulatory in coleoptile elongation and plant growth in the seedling stage, respectively [56,57]. …”

Proteomics (SWATH-MS) informed by transcriptomics approach of tropical herbPersicaria minorleaves upon methyl jasmonate elicitation

“…This suggests that the enzyme regulates positive feedbacks of jasmonate production during an injury response or stress. Furthermore, GER1 gene (GDSL containing enzyme rice 1) containing a GDSL motif was responsible for the light regulated JA response (Riemann et al, 2007), suggesting that the GDSL esterase/lipase could also be part of the defense mechanism mediated by jasmonate (Chepyshko et al, 2012). …”

Screening of UV-B-induced genes from apple peels by SSH: possible involvement of MdCOP1-mediated signaling cascade genes in anthocyanin accumulation

“…RBX1 (RING box protein 1) E3 ubiquitin ligase (13B5) plays a pivotal role in the maintenance of genome integrity (Jia et al ), alleviating cells from the genetic damage caused by UV‐B irradiation (Rozema et al ). GDSL‐motif lipases (GDSLs and 13A11) have been studied in various plants and function mainly in the regulation of plant development and defense response involved in jasmine acid, ethylene, light and auxin signaling (Oh et al , Ling et al , Riemann et al , Kwon et al , Lee et al ). The expression of GDSL ( 13A11 ) is UV‐B inducible in both ‘Mutsu’ and ‘Tsugaru’ (data not shown).…”