Ternary copper(II) complexes with amino acid chains and heterocyclic bases: DNA binding, cytotoxic and cell apoptosis induction properties

Ma, Tieliang; Xu, Jun; Wang, Yuan; Yu, Hao; Yang, Yanyan; Liu, Yang; Ding, Weiliang; Zhu, Wei; Chen, Ruhua; Zhang, Ge; Tan, Yongfei; Jia, Lei; Zhu, Tao

doi:10.1016/j.jinorgbio.2014.12.011

Cited by 65 publications

(24 citation statements)

References 53 publications

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“…The better cytotoxic activity of complex 4 is in accordance with its stronger DNA‐binding ability, which further suggests the binding of the complexes to DNA and thus consequently leads to cell death. The result obtained here is similar to the published work by us recently , while the cytotoxicity of the complexes here is slightly higher than the published results. The morphology examinations also show that the proliferation of the cells is significantly inhibited and the cells exhibit morphological changes such as cell shrinkage and cell detachment, as shown in Figure .…”

Section: Resultssupporting

confidence: 92%

Ternary Dinuclear Copper(II) Complexes of a Reduced Schiff Base Ligand with Diimine Coligands: DNA Binding, Cytotoxic Cell Apoptosis, and Apoptotic Mechanism

Yang

et al. 2015

Chem Biol Drug Des

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A serial of mixed-ligand Cu(II) complexes of the type [Cu(phens)(H2 PDILeu)]H2 O (1-4) containing phens as 2,2'-bipyridyl (bpy, 1), 1,10-phenanthroline (phen, 2), dipyrido[3,2-d:2',3'-f]quinoxaline (dpq, 3), and dipyrido[3,2-a:2',3'-c]phenazine (dppz, 4) have been isolated and characterized. The interaction of the complexes with calf-thymus DNA has been explored by physical methods to propose modes of DNA binding of the complexes, which indicate that 4 interacts with DNA more strongly than all of the other complexes through intercalation interaction. Furthermore, cell apoptosis was detected by AnnexinV/PI flow cytometry and TUNEL assay and by Western blotting to detect the protein expression of p53, Bax, and Bcl-2. All the three copper complexes can effectively induce apoptosis of the three human tumor cells, which was accompanied with upregulation of the expression of p53 and Bax, while Bcl-2 decreased.

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Section: Resultssupporting

confidence: 92%

Ternary Dinuclear Copper(II) Complexes of a Reduced Schiff Base Ligand with Diimine Coligands: DNA Binding, Cytotoxic Cell Apoptosis, and Apoptotic Mechanism

Yang

et al. 2015

Chem Biol Drug Des

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“…Growing evidence indicates that apoptosis is an active physiological process of cell destruction that involves specific morphological and biochemical changes in the nucleus and cytoplasm and the fragmentation of DNA is one of the hallmarks of apoptosis (Ma et al, 2015). In this study, apoptosis was observed by Hoechst 33258-stained nuclei in a fluorescence microscope.…”

Section: Discussionmentioning

confidence: 87%

Platycodin D exerts anti-tumor efficacy in H22 tumor-bearing mice via improving immune function and inducing apoptosis

Tian

Liu

et al. 2016

J. Toxicol. Sci.

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-Platycodin D (PD), a major saponin derived and isolated from the roots of Platycodon grandiflorum, exerts potent growth inhibition and strong cytotoxicity against various cancer cell lines. However, the anti-tumor efficacy of PD on H22 hepatocellular carcinoma remains unknown. In the present study, we aimed to explore the anti-hepatoma activity in vivo and the underlying mechanism of PD in H22 tumor-bearing mice. The results revealed that PD could considerably suppress tumor growth with no significant side effects on immune organs and body weight. Further investigations showed that the levels of serum cytokines, including interferon gamma (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-2 (IL-2), were enhanced by PD administration. On the other hand, PD inhibited the production of vascular endothelial growth factor (VEGF) in serum of H22 tumor mice. Additionally, the observations from H&E and Hoechst 33258 staining results demonstrated that PD noticeably induced apoptosis in H22 hepatocellular carcinoma cells. Importantly, immunohistochemical analysis showed that PD treatment increased Bax expression and decreased Bcl-2 and VEGF expression of H22 tumor tissues in a dose-dependent manner. Taken together, the findings in the present investigation clearly demonstrated that the PD markedly suppressed the tumor growth of H22 transplanted tumor in vivo at least partly via improving the immune functions, inducing apoptosis, and inhibiting angiogenesis.

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“…34 To further confirm that the NCs have no effect on the growth of human liver cells, Annexin V/PI staining and flow cytometry were used to quantify cell apoptosis. 35 Treatment with NCs at a high concentration (200 µg/mL) had no effect on the number of apoptotic LO2 cells detected by flow cytometry, indicating that the prepared NCs were suitable for biological applications (Figure 8). …”

Section: Cell Cytotoxicity and Apoptosismentioning

confidence: 98%

Multifunctional nanocomposite based on halloysite nanotubes for efficient luminescent bioimaging and magnetic resonance imaging

Zhou¹,

Jia²,

Luo³

et al. 2016

IJN

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A novel multifunctional halloysite nanotube (HNT)-based Fe 3 O 4 @HNT-polyethyleneimine-Tip-Eu(dibenzoylmethane) 3 nanocomposite (Fe-HNT-Eu NC) with both photoluminescent and magnetic properties was fabricated by a simple one-step hydrothermal process combined with the coupling grafting method, which exhibited high suspension stability and excellent photophysical behavior. The as-prepared multifunctional Fe-HNT-Eu NC was characterized using various techniques. The results of cell viability assay, cell morphological observation, and in vivo toxicity assay indicated that the NC exhibited excellent biocompatibility over the studied concentration range, suggesting that the obtained Fe-HNT-Eu NC was a suitable material for bioimaging and biological applications in human hepatic adenocarcinoma cells. Furthermore, the biocompatible Fe-HNT-Eu NC displayed superparamagnetic behavior with high saturation magnetization and also functioned as a magnetic resonance imaging (MRI) contrast agent in vitro and in vivo. The results of the MRI tests indicated that the Fe-HNT-Eu NC can significantly decrease the T 2 signal intensity values of the normal liver tissue and thus make the boundary between the normal liver and transplanted cancer more distinct, thus effectively improving the diagnosis effect of cancers.

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Ternary copper(II) complexes with amino acid chains and heterocyclic bases: DNA binding, cytotoxic and cell apoptosis induction properties

Cited by 65 publications

References 53 publications

Ternary Dinuclear Copper(II) Complexes of a Reduced Schiff Base Ligand with Diimine Coligands: DNA Binding, Cytotoxic Cell Apoptosis, and Apoptotic Mechanism

Ternary Dinuclear Copper(II) Complexes of a Reduced Schiff Base Ligand with Diimine Coligands: DNA Binding, Cytotoxic Cell Apoptosis, and Apoptotic Mechanism

Platycodin D exerts anti-tumor efficacy in H22 tumor-bearing mice via improving immune function and inducing apoptosis

Multifunctional nanocomposite based on halloysite nanotubes for efficient luminescent bioimaging and magnetic resonance imaging

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