Termiticidal activity of lectins from Myracrodruon urundeuva against Nasutitermes corniger and its mechanisms

Napoleão, Thiago Henrique; Gomes, Francis Soares; Lima, Tânia Andrade; Santos, Nataly Diniz de Lima; Sá, Roberto Araújo; Albuquerque, Auristela C.; Coelho, Luana Cassandra Breitenbach Barroso

doi:10.1016/j.ibiod.2010.05.015

Cited by 50 publications

(50 citation statements)

References 66 publications

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“…The three lectins agglutinated human and rabbit erythrocytes in a broad pH range, and the hemagglutinating activities were inhibited by N-acetylglucosamine. SDS-PAGE revealed that MuBL, MuHL and MuLL are polypeptides of 14, 14.4 and 14.2 kDa, respectively (Sá et al, 2009c;Napoleão et al, 2011). MuHL showed antimicrobial activity inhibiting the growth of numerous bacteria (Bacillus subtilis, Corynebacterium callunae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus faecalis) and fungi (Fusarium oxysporum, F. decemcellulare, F. fusarioides, F. solani and F. verticilioides (Sá et al, 2009b).…”

Section: Lectins From Myracrodruon Urundeuva Bark Heartwood and Leafmentioning

confidence: 99%

“…Lectins have deleterious effects against larvae, developing stages and mature forms of insects from orders Coleoptera, Diptera, Hemiptera, Homoptera, Hymenoptera, Isoptera, Lepidoptera and Neuroptera (Murdock et al, 1990;Eisemann et al, 1994;Powell et al, 1995;Zhu-Salzman et al, 1998;Bandyopadhyay et al, 2001;Isidro et al, 2001;Hogervorst et al, 2006;Kaur et al, 2006;Coelho et al, 2007;Macedo et al, 2007;Fitches et al, 2008;Sá et al, 2008;Coelho et al, 2009;Sá et al, 2009c;Silva et al, 2009;Napoleão et al, 2011;Souza et al, 2011b). Insecticide activity of lectin is generally evaluated by bioassays that incorporate the lectin into artificial diets offered to insects, with insects dying from nutritional deprivation.…”

Section: Insecticidal Activity Of Lectinsmentioning

confidence: 99%

“…Insecticide activity of lectin is generally evaluated by bioassays that incorporate the lectin into artificial diets offered to insects, with insects dying from nutritional deprivation. It has been shown that lectins are resistant to proteases present in the insect gut, a property responsible for their active presence in the digestive tract, eventually with insecticide effects Napoleão et al, 2011;Oliveira et al, 2011). The precise mechanisms of insecticidal action of lectins remain unknown, though it has been suggested that this entomotoxic activity seems to depend upon the carbohydrate recognition property they exhibit.…”

Section: Insecticidal Activity Of Lectinsmentioning

confidence: 99%

“…That study suggested that resistance of the heartwood to termite attack may be linked to termiticidal activity of MuHL. The termiticidal effect of lectins isolated from M. urundeuva bark and leaf (Table 1) was determined by Napoleão et al (2011). Both MuBL and MuLL killed workers (LC 50 of 0.974 and 0.374 mg ml -1 , respectively) and soldiers (LC 50 of 0.787 and 0.432 mg ml -1 , respectively).…”

Section: Termiticidal Activity Of Lectins Against Nasutitermes Cornigermentioning

confidence: 99%

“…Lectin solubility and stability vary with the sequence of amino acids in the polypeptide chain, and such structural features can be exploited to provide concentrated lectin preparations. Lectins can be precipitated from extracts by adding ammonium sulfate at high concentration (salting out method) or organic solvents (Santana et al, 2008;Napoleão et al, 2011). Heat-stable lectins can be partially purified by submitting the extract to high temperature for removal of other proteins (Santana et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Insecticide Activity of Lectins and Secondary Metabolites

Paiva¹,

Napoleão²,

Sá³

et al. 2012

Insecticides - Advances in Integrated Pest Management

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Section: Lectins From Myracrodruon Urundeuva Bark Heartwood and Leafmentioning

confidence: 99%

Section: Insecticidal Activity Of Lectinsmentioning

confidence: 99%

Section: Insecticidal Activity Of Lectinsmentioning

confidence: 99%

Section: Termiticidal Activity Of Lectins Against Nasutitermes Cornigermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Insecticide Activity of Lectins and Secondary Metabolites

Paiva¹,

Napoleão²,

Sá³

et al. 2012

Insecticides - Advances in Integrated Pest Management

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EFFECT OF Moringa oleifera FLOWER EXTRACT ON LARVAL TRYPSIN AND ACETHYLCHOLINESTERASE ACTIVITIES IN Aedes aegypti

Pontual

Napoleão

Assis

et al. 2012

Arch Insect Biochem Physiol

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Aedes aegypti control is crucial to reducing dengue fever. Aedes aegypti larvae have developed resistance to organophosporous insecticides and the use of natural larvicides may help manage larval resistance by increasing elements in insecticide rotation programs. Here, we report on larvicidal activity of Moringa oleifera flower extract against A. aegypti L(1), L(2), L(3), and L(4) as well as the effect of flower extract on gut trypsin and whole-larval acetylcholinesterase from L(4.) In addition, the heated flower extract was investigated for larvicidal activity against L(4) and effect on larval gut trypsin. Moringa oleifera flower extract contains a proteinaceous trypsin inhibitor (M. oleifera flower trypsin inhibitor, MoFTI), triterpene (β-amyrin), sterol (β-sitosterol) as well as flavonoids (kaempferol and quercetin). Larvicidal activity was detected against L(2), L(3), and L(4) (LC(50) of 1.72%, 1.67%, and 0.92%, respectively). Flower extract inhibited L(4) gut trypsin (MoFTI K(i) = 0.6 nM) and did not affect acetylcholinesterase activity. In vivo assay showed that gut trypsin activity from L(4) treated with M. oleifera flower extract decreased over time (0-1,440 min) and was strongly inhibited (98.6%) after 310 min incubation; acetylcholinesterase activity was not affected. Thermal treatment resulted in a loss of trypsin inhibitor and larvicidal activities, supporting the hypothesis that flower extract contains a proteinaceous trypsin inhibitor that may be responsible for the deleterious effects on larval mortality.

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Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

Nova

Almeida

Procópio

et al. 2022

Arch Insect Biochem Physiol

Self Cite

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This study evaluated the effects of acute exposure of Aedes aegypti third instar (L3) larvae to the saline extract of Opuntia ficus‐indica cladodes on the biological cycle and fertility of the emerging adults. For this, larvae were treated for 24 h with the extract at ¼ LC50 (lethal concentration to kill 50% of larvae), ½ LC50 or LC50; the development and reproduction of the emerged adults were evaluated after a recovery period of 9 days. The resistance of proteins in the extract to hydrolysis by L3 digestive enzymes and histomorphological alterations in the larval midgut were also investigated. The extract contained lectin, flavonoids, cinnamic derivatives, terpenes, steroids, and reducing sugars. It showed a LC50 of 3.71% for 48 h. The data indicated mean survival times similar in control and extract treatments. It was observed development delay in extract‐treated groups, with a lower number of adults than in control. However, the females that emerged laid similar number of eggs in control and treatments. Histological evaluation revealed absence of bacterial and fungal microorganisms in the food content in midguts from larvae treated with cladode extract. Electrophoresis revealed that three polypeptides in the extract resisted to hydrolysis by L3 digestive proteases for 90 min. The lectin activity was not altered even after 24‐h incubation with the enzymes. In conclusion, the extract from O. ficus‐indica can delay the development of Ae. aegypti larvae, which may be linked to induction of an axenic environment at larval midgut and permanence of lectin activity even after proteolysis.

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Termiticidal activity of lectins from Myracrodruon urundeuva against Nasutitermes corniger and its mechanisms

Cited by 50 publications

References 66 publications

Insecticide Activity of Lectins and Secondary Metabolites

Insecticide Activity of Lectins and Secondary Metabolites

EFFECT OF Moringa oleifera FLOWER EXTRACT ON LARVAL TRYPSIN AND ACETHYLCHOLINESTERASE ACTIVITIES IN Aedes aegypti

Extract from Opuntia ficus‐indica cladode delays the Aedes aegypti larval development by inducing an axenic midgut environment

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