Termiticidal Activity and Chemical Components of Bamboo Vinegar against Subterranean Termites under Different Pyrolysis Temperatures

Subterranean termite attacks on cajuput (Melaleuca cajuputi) seedling roots were widespread in several of Java's Perum Perhutani Forest Management Units. This attack was suspected to be related to the chemical components of the cajunput seedling roots. This study was conducted to determine the bioactivity of cajuput seedling root extract as an attractant for the subterranean termite Coptotermes curvignathus Holmgren (Isoptera: Rhinotermitidae). The extraction process was performed according to ASTM D1108-96, and the extract was characterized using gas chromatography-mass spectrometry (GC-MS). Bioactivity testing of the extracts was carried out using attractiveness and no-choice feeding bioassays. The results showed that the average root extraction yield from cajunput seedlings was 4.94%. The attractiveness of the extract solutions at concentrations of 0.50%, 0.75%, and 1.00% were 45.33%, 62.00%, and 74.67%, respectively. The mortality rate of C. curvignathus termites ranged from 9.63% to 24.44%. Cajuput seedling root extract's lethal concentration 50 was 2.45% (non-toxic). GC-MS analysis showed that the extract contained linoleic acid, which has the potential to attract insects.

“…4 shows that the greater the concentration, the more termites died. (Arsyad et al, 2020;Oramahi et al, 2022;Suprianto et al, 2023).…”

Section: Mortality Of Subterranean Termitesmentioning

confidence: 99%

Bioactivity of Cajuput Seedling n-Hexane Extract as an Attractant for Subterranean Termite Coptotermes curvignathus Holmgren (Isoptera: Rhinotermitidae)

ARINANA,

DHARMA,

SARI

et al. 2024

“…Termites are wood-eating pests (Arinana et al, 2022;Su and Scheffrahn, 2000). Since termites damage wooden buildings, many studies have been conducted to control them in various ways (Adfa et al, 2020(Adfa et al, , 2023Arsyad et al, 2020;Cahyono et al, 2020;Ella Nkogo et al, 2022;Im and Han, 2020;Lee et al, 2020;Oramahi et al, 2022;Priadi et al, 2023). However, they also serve as ecological suppliers providing various nu-trients, including nitrogen compounds (Govorushko, 2019;Lobry de Bruyn and Conacher, 1990).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Low-Temperature Enzymatic Reactions through Heterologous Expression and Functional Analysis of Two Beta-Glucosidases from the Termite Symbiotic Bacterium Elizabethkingia miricola Strain BM10

LEE,

KIM

2023

Lower termites need symbiotic microbes for cellulose digestion. Elizabethkingia miricola strain BM10 has been proposed as a symbiotic microbe that assists in low-temperature digestion and metabolism of Reticulitermes speratus KMT1, a termite on Bukhan Mountain, Seoul, Korea. In E. miricola strain BM10, β-glucosidase genes expressed at 10℃ were identified, and the psychrophilic enzymatic characteristic was confirmed by heterogeneously expressed proteins. Crude β-glucosidase in the culture broth of E. miricola strain BM10 showed specific enzymatic properties, and its substrate affinity was 4.69 times higher than that of Cellic CTec2. Among the genes proposed as β-glucosidase, two genes, bglB_1 and bglA_2, whose gene expression was more than doubled at 10℃ than at 30℃, were identified. They were heterogeneously expressed in Escherichia coli and identified as psychrophilic enzymes with an optimal reaction temperature of about 20℃-25℃. In this study, E. miricola strain BM10, a symbiotic bacterium of lower termites, produced psychrophilic β-glucosidases that contribute to the spread of the low-temperature habitat of a lower termite, R. speratus KMT1.

“…The wood vinegar contains primary chemical components such as acids, ketones, furans, benzene, phenols, sugars, and guaiacols. Arsyad et al (2020) reported that wood vinegar obtained from bamboo pyrolyzed at 400℃ contains phenols and acids that exhibit antitermitic activity.…”

Section: Introductionmentioning

confidence: 99%

The Antitermitic and Antifungal Activities and Composition of Vinegar from Durian Wood (Durio sp.)

SUPRIANTO,

ORAMAHI,

DIBA

et al. 2023

Chemical characterization of vinegars obtained from Durian wood (Durio sp.) and their termicidal activity against Coptotermes curvignathus and antifungal activity against Schizophyllum commune were evaluated. The process of pyrolysis produced wood vinegars at three distinct temperature: 350℃, 400℃, and 450℃. To determine their effectiveness against fungal growth, the vinegars were tested using a Petri dish with 1.0%, 2.0%, 3.0%, and 4.0% (v/v) against S. commune. In the experiment, termicidal activities were evaluated using a no-choice test for C. curvignathus with 3.0%, 6.0%, 9.0%, and 12.0% (v/v). The wood vinegar exhibited antitermitic activity to C. curvignathus workers in the no-choice experiment; For vinegar produced at 450℃, a 6% concentration was required to achieve 100% mortality against C. curvignathus. In addition, a 12% vinegar produced at 450℃ resulted in the lowest mass loss of treated filter paper, which was 20.00%. Furthermore, all the wood vinegars exhibited antifungal activities against S. commune at concentration of 2.0%. The dominant chemical components of wood vinegar produced at temperature of 350℃, 400℃, and 450℃ were 2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 4-ethyl-2-methoxy-phenol, 3.5-dimethoxy-4-hydroxytoluene, and creosol.