Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro

Alviano, Francesco; Fossati, Valentina; Marchionni, Cosetta; Arpinati, Mario; Bonsi, Laura; Franchina, Michele; Lanzoni, Giacomo; Cantoni, Silvia; Cavallini, Claudia; Bianchi, Francesca; Tazzari, Pier Luigi; Pasquinelli, Gianandrea; Foroni, Laura; Ventura, Carlo; Grossi, Alberto; Bagnara, Gian Paolo

doi:10.1186/1471-213x-7-11

Cited by 330 publications

(114 citation statements)

References 51 publications

(52 reference statements)

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“…The results of this study show that CD105-, CD29-positive and CD34-negative MSCs present in the major components of human umbilical cord (AM and WJ, UA and UV) and this finding is supported by some previous studies [1,4,5,[25][26][27]. It was an interesting observation that endothelial lining of both the UA and UV showed expression of the MSCs markers CD29 and CD105.…”

Section: Discussionsupporting

confidence: 88%

Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

Alrefaei

Ayuob

Ali

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Although the human umbilical cord (UC) has been previously considered a medical waste, its use as a main source of fetal stem cells for regenerative medicine applications has increased over the past few years. The aim of the study was to assess the impact of the maternal age on the expression of mesenchymal stem cells (MSC) markers CD105 and CD29 in the different areas of human UC. Material and methods. In this comparative cross sectional study, one hundred term UCs from five maternal age groups (20-45 years) were collected after delivery from healthy mothers and were processed to assess both immuno-and gene expression of CD105 and CD29 surface antigen markers using immunohistochemical and RT-PCR techniques. Results. The immunoexpression of CD105 and CD29 in the amniotic membrane (AM) and Wharton's jelly (WJ), the umbilical artery (UA) and the umbilical vein (UV) showed significant negative correlation with the maternal age (p < 0.001). Reduced amount of cells as well as the studied MSC markers and their gene expression levels were documented in older age mothers. CD105-positive MSCs were more abundant in the UA, whereas CD29-positive MSCs were more abundant in the AM and WJ. Conclusion. The decreased expression of CD105 and CD29 MSCs markers with age suggests that selective isolation of MSCs from Wharton's jelly, umbilical artery or umbilical vein of younger mothers should be recommended.

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Section: Discussionsupporting

confidence: 88%

Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

Alrefaei

Ayuob

Ali

et al. 2015

Folia Histochem Cytobiol.

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“…Recently, multipotent MSCs have also been isolated in placental tissues, including the amnion and chorion layers of the amniotic membrane,21, 22, 23, 24 and these amniotic membrane derived stem cells have demonstrated the ability to regulate immune cells though secretion of various immunomodulatory cytokines 25, 26, 27, 28…”

Section: Introductionmentioning

confidence: 99%

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

Massee

Chinn

Lei³

et al. 2015

J Biomed Mater Res

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Human‐derived placental tissues have been shown in randomized clinical trials to be effective for healing chronic wounds, and have also demonstrated the ability to recruit stem cells to the wound site in vitro and in vivo. In this study, PURION® Processed dehydrated human amnion/chorion membrane allografts (dHACM, EpiFix®, MiMedx Group, Marietta, GA) were evaluated for their ability to alter stem cell activity in vitro. Human bone marrow mesenchymal stem cells (BM‐MSCs), adipose derived stem cells (ADSCs), and hematopoietic stem cells (HSCs) were treated with soluble extracts of dHACM tissue, and were evaluated for cellular proliferation, migration, and cytokine secretion. Stem cells were analyzed for cell number by DNA assay after 24 h, closure of an acellular zone using microscopy over 3 days, and soluble cytokine production in the medium of treated stem cells was analyzed after 3 days using a multiplex ELISA array. Treatment with soluble extracts of dHACM tissue stimulated BM‐MSCs, ADSCs, and HSCs to proliferate with a significant increase in cell number after 24 h. dHACM treatment accelerated closure of an acellular zone by ADSCs and BM‐MSCs after 3 days, compared to basal medium. BM‐MSCs, ADSCs, and HSCs also modulated endogenous production of a number of various soluble signals, including regulators of inflammation, mitogenesis, and wound healing. dHACM treatment promoted increased proliferation and migration of ADSCs, BM‐MSCs, and HSCs, along with modulation of secreted proteins from those cells. Therefore, dHACM may impact wound healing by amplifying host stem cell populations and modulating their responses in treated wound tissues. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1495–1503, 2016.

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“…AM-MSCs are easily isolated through mechanical and sequential trypsin and collagenase digestion, in a significant number, approximately 2x10 6 per gram of the amnion [45]. They exhibit a fibroblast-like morphology and have a capability to differentiate towards not only adipogenic and osteogenic, but also chondrogenic, skeletal myogenic and endothelial lineages [46]. AM-MSCs have the capacity to support the hematopoiesis of CD34 + cells in co-cultures, even in the absence of exogenous cytokines.…”

Section: Amniotic Membrane -Human Mesenchymal Stem/stromal Cells (Am-mentioning

confidence: 99%

“…Alviano et al showed that AM-MSCs spontaneously form capillary-like structures when they are cultured in semisolid medium. Supplementing the culture medium with vascular endothelial growth factor (VEGF) strengthens the AM-MSCs angiogenic behavior [46]. AM-MSCs are capable of secreting several growth factors that support angiogenesis and tissue remodeling, and decrease inflammation, such as: transforming growth factor-β (TGF-β), basic fibroblast growth factor (FGF), epidermal growth factor (EGF), keratinocyte growth factor, and hepatocyte growth factor (HGF) [48].…”

Section: Amniotic Membrane -Human Mesenchymal Stem/stromal Cells (Am-mentioning

confidence: 99%

“…When the C-MSCs were cultured in differentiation media, they demonstrated broad morphogenetic plasticity and differentiated into osteoblasts, osteocytes, chondrocytes, myocytes, adipocytes, and neural cells [63,64]. They can also differentiate into hepatocytes, endothelial cells, and cardiomyocytes [46,67,68]. Despite the high plasticity of these cells, there is little experience with chorionderived stem cells, which may be related to their poor survival in advanced passages.…”

Section: Chorionic Mesenchymal Stem/stromal Cells (C-mscs)mentioning

confidence: 99%

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Perinatal sources of mesenchymal stem cells: Wharton’s jelly, amnion and chorion

Witkowska-Zimny

Wróbel

2011

Cellular and Molecular Biology Letters

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Recently, stem cell biology has become an interesting topic, especially in the context of treating diseases and injuries using transplantation therapy. Several varieties of human stem cells have been isolated and identified in vivo and in vitro. Ideally, stem cells for regenerative medical application should be found in abundant quantities, harvestable in a minimally invasive procedure, then safely and effectively transplanted to either an autologous or allogenic host. The two main groups of stem cells, embryonic stem cells and adult stem cells, have been expanded to include perinatal stem cells. Mesenchymal stem cells from perinatal tissue may be particularly useful in the clinic for autologous transplantation for fetuses and newborns, and after banking in later stages of life, as well as for in utero transplantation in case of genetic disorders.This review highlights the characteristics and therapeutic potential of three human mesenchymal stem cell types obtained from perinatal sources: Wharton’s jelly, the amnion, and the chorion.

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Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro

Cited by 330 publications

References 51 publications

Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

Effects of maternal age on the expression of mesenchymal stem cell markers in the components of human umbilical cord

Dehydrated human amnion/chorion membrane regulates stem cell activity in vitro

Perinatal sources of mesenchymal stem cells: Wharton’s jelly, amnion and chorion

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