Terbium-Sensitized Fluorescence Method for the Determination of Pazufloxacin Mesilate and Its Application

Abstract

A simple, rapid and highly sensitive fluorometric method for the determination of pazufloxacin mesilate (PZFX) is described. It is based on the formation of the complex [Tb(PZFX)2] 3+ , which shows the intensive characteristic bands of Tb 3+ . Optimum conditions for the determination were investigated. Under the optimum experimental condition, the fluorescence intensity responds linearly to the PZFX concentration in the range of 2.0 × 10 -8 -5.0 × 10 -6 mol/l with a detection limit of 6.2 × 10 -9 mol/l. The method has been successfully applied to the determination of PZFX in urine and serum samples.

A capacitive sensor based on molecularly imprinted polymers and poly(p-aminobenzene sulfonic acid) film for detection of pazufloxacin mesilate

“…The detection limit corresponding to three times the standard deviation of the blank solution was estimated as 1.8 ng·mL −1 . Compared with those of conventional chemiluminescence methods [3][4][5] , the proposed capacitive sensor showed the lower detection limit.…”

“…This compound is currently widely used for the treatment of infections, such as respiratory organs infection, genitourinary organ infection, skin and parenchyma infection, and surgical infection, which is caused by Gram-negative and positive bacteria [2] . The chemical structure of pazufloxacin is shown in Figure 1.Chemiluminescence and chromatography methods were employed for the determination of pazufloxacin [3][4][5] . Figure 1 The structure of pazuflouxacin mesilate.…”

“…Chemiluminescence and chromatography methods were employed for the determination of pazufloxacin [3][4][5] . However, these methods have suffered several disadvantages, such as low sensitivity and long detection period.…”

Spectrophotometric studies on the interaction between pazufloxacin mesilate and human serum albumin or lysozyme

“…Pazufloxacin mesilate (PZFX, (À)-(S)-10-(1-aminocyclopropyl)-9-fluoro-3-methyl-7-oxo-2,3-dihydro-7H-pyrido [1,2,3-de] [1,4]benzoxazine-6-carboxylic acid monome thanesulfonate) (see Fig. 1) is a newly developed quinolone antimicrobial agent.…”

Synthesis, characterization and biological aspects of novel five-coordinated dimeric-Cu(II) systems

“…Several spectrophotometry methods have been reported for the determination of fluoroquinolones in human blood and urine via metal chelation [2][3][4]. There is a drastic change in the biochemical properties of copper on chelation with drugs and it possess modified toxicological and pharmacological properties.…”