Spectrophotometric studies on the interaction between pazufloxacin mesilate and human serum albumin or lysozyme

Jin, Jing; Zhang, Xia

doi:10.1016/j.jlumin.2007.05.008

Cited by 57 publications

(25 citation statements)

References 25 publications

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“…From the above equation, the a-helical structure of HSA is increased from 48.73% to 50.32% and 52.64% at the molar ration ratio of HSA to docetaxel of 1:6 and 1:12. The percentage of a-helical structure in HSA increased indicates that docetaxel strengthened their hydrogen bonding networks and stabilized helical structures in protein upon the addition of docetaxel [35,36]. These results further verify hydrogen bonds play a major role in binding and are consistent with the thermodynamic analysis.…”

supporting

confidence: 83%

Interaction of the docetaxel with human serum albumin using optical spectroscopy methods

Cheng

Liu

Zhang

et al. 2009

Journal of Luminescence

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supporting

confidence: 83%

Interaction of the docetaxel with human serum albumin using optical spectroscopy methods

Cheng

Liu

Zhang

et al. 2009

Journal of Luminescence

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“…on BSA When small molecules bind independently to a set of equivalent sites on a macromolecule, the equilibrium between free and bound molecules is given by the following equation [22]: than 0.999, and the standard deviation is no more than 0.010, indicating that the assumptions underlying the derivation of Eq. 5 are satisfied.…”

Section: Number Of Binding Sites and Identification Of The Binding Lomentioning

confidence: 99%

Spectroscopic Studies on the Binding of Cobalt(II) 1,10-Phenanthroline Complex to Bovine Serum Albumin

Zhang

Dai

et al. 2009

Biol Trace Elem Res

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The binding interaction of the cobalt(II) 1,10-phenanthroline complex (Co(phen) (3) (2+) , phen = 1,10-phenanthroline) with bovine serum albumin (BSA) was investigated by fluorescence spectroscopy combined with UV-Vis absorption and circular dichroism measurements under simulative physiological conditions. The experiment results showed that the fluorescence intensity of BSA was dramatically decreased owing to the formation of Co(phen) (3) (2+) -BSA complex. The corresponding association constants (K (a)) between Co(phen) (3) (2+) and BSA at four different temperatures were calculated according to the modified Stern-Volmer equation. The enthalpy change (DeltaH degrees ) and entropy change (DeltaS degrees ) were calculated to be -2.73 kJ mol(-1) and 82.27 J mol(-1) K(-1), respectively, which suggested that electrostatic interaction and hydrophobic force played major roles in stabilizing the Co(phen) (3) (2+) -BSA complex. Site marker competitive experiments indicated that the binding of Co(phen) (3) (2+) to BSA primarily took place in site I of BSA. A value of 4.11 nm for the average distance r between Co(phen) (3) (2+) (acceptor) and tryptophan residues of BSA (donor) was derived from Förster's energy transfer theory. The conformational investigation showed that the presence of Co(phen) (3) (2+) resulted in the change of BSA secondary structure and induced the slight unfolding of the polypeptides of protein, which confirmed the microenvironment and conformational changes of BSA molecules.

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“…Meanwhile, an increase in the fluorescence intensity at 378 nm could be assigned to PZFX. 29 From the fluorescence emission spectra, we could conclude that AuNC@BSA and PZFX had a strong interaction.…”

Section: Response Mechanism Of Auncs@bsa To Pzfxmentioning

confidence: 89%

A Fluorescence Probe Based on Biomolecule-stabilized Gold Nanoclusters for the Detection of Pazufloxacin Mesilate

Cao

Lian

et al. 2014

ANAL. SCI.

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Spectrophotometric studies on the interaction between pazufloxacin mesilate and human serum albumin or lysozyme

Cited by 57 publications

References 25 publications

Interaction of the docetaxel with human serum albumin using optical spectroscopy methods

Interaction of the docetaxel with human serum albumin using optical spectroscopy methods

Spectroscopic Studies on the Binding of Cobalt(II) 1,10-Phenanthroline Complex to Bovine Serum Albumin

A Fluorescence Probe Based on Biomolecule-stabilized Gold Nanoclusters for the Detection of Pazufloxacin Mesilate

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