Teratogenic and lethal effects of paraquat on developing frog embryos (Rana pipiens)

Dial, Norman A.; Bauer, Cheryl A.

doi:10.1007/bf01625589

Cited by 30 publications

(9 citation statements)

References 4 publications

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“…In an experiment with fathead minnows exposed to Aroclor 1242 (Monsanto, St. Louis, MO, USA) a commercial PCB mixture, eggs maintained at high concentrations (15 to 51 g/L) hatched with good success, but none of the fry survived [17]. Dial and Bauer [34] found leopard frog eggs to be very resistant to paraquat, a herbicide, until approximately 3 d posthatch, when a significant increase in mortality was observed in all but one treatment group. Berrill et al [35] also found that in experimental exposures of leopard and green frogs to low concentrations of pyrethroid insecticides, ''newly hatched tadpoles are considerably more sensitive than embryo stages.…”

Section: Embryos and Larvaementioning

confidence: 99%

Effects of Polychlorinated Biphenyl 126 on Green Frog(rana Clamitans) and Leopard Frog (Rana Pipiens)hatching Success, Development, and Metamorphosis

Rosenshield¹,

Jofré²,

Karasov³

1999

Environ Toxicol Chem

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Although increasing evidence links planar chlorinated hydrocarbons, such as polychlorinated biphenyls (PCBs), to decreases in survival and reproduction of fish, mammals, and birds near Green Bay, Wisconsin, and the Great Lakes, USA, relatively little is known of their bioaccumulation or of their possible effects in amphibians. We exposed embryos and larvae of two ranid species commonly occurring in the Green Bay ecosystem, the green frog (Rana clamitans) and the leopard frog (Rana pipiens), to PCB 126, a model coplanar PCB compound. Nominal concentrations ranged from 0.005 to 50 g/L, and exposure lasted through metamorphosis. Tissue concentrations of PCB 126 in tadpoles that did not metamorphose by the end of the experiment ranged from 1.2 to 9,600 ng/g wet mass. No significant mortality of embryos occurred before hatching; however, survival of larvae was significantly reduced at the highest concentration for both species. Few deformities were observed, but the incidence of edema was significantly higher in tadpoles exposed to 50 g/L. Swimming speed and growth of tadpoles was also significantly reduced in this treatment. The percent of tadpoles that reached metamorphosis was significantly lower in green frogs at the highest concentration, and no leopard frogs survived past day 47 of the experiment in this treatment. At high concentrations, PCB 126 affected both ranid species; however, sublethal effects were not apparent for the parameters we measured at concentrations that occur in water in the Green Bay ecosystem.

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Section: Embryos and Larvaementioning

confidence: 99%

Effects of Polychlorinated Biphenyl 126 on Green Frog(rana Clamitans) and Leopard Frog (Rana Pipiens)hatching Success, Development, and Metamorphosis

Rosenshield¹,

Jofré²,

Karasov³

1999

Environ Toxicol Chem

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“…Similarly, exposure of B. melanostictus, Polypedates cruciger and Rana clamitans larvae to OP diazinon leads to tail curving and bending (Harris et al 1998;Ratnasooriya 2008a, 2008b). Tail abnormalities (bent or drooped or narrow margined tails) and stunted growth were Toxicological & Environmental Chemistry 2017 observed in Rana pipiens tadpoles exposed to paraquat and chlorpyrifos (Dial and Bauer 1984;Gaizick, Gupta and Bass 2001). The tail deformity of larvae is attributed to the failure of expansion of the vitelline membrane surrounding the embryo that forces the growing spine to curl (Langan 2003).…”

Section: Discussionmentioning

confidence: 97%

Morphological and behavioral alterations induced by endocrine disrupters in amphibian tadpoles

Ghodageri

Pancharatna

2011

Toxicological & Environmental Chemistry

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Developmental toxicity effects of endocrine disrupter chemicals, acephate and cypermethrin were studied in Bufo melanostictus tadpoles. Thirty developing eggs of B. melanostictus were exposed to each concentration (0.01, 0.05, 0.1, 0.5, and 1 mg L À1 ) of acephate or cypermethrin in the laboratory (temperature: 23 AE 1 C; photoperiod: 11.5-12.5 h). Eggs maintained in conditioned water alone served as controls. After hatching, larvae were fed on boiled spinach until the completion of metamorphosis. In control group, larvae that hatched on 3rd day were heavily pigmented, voracious feeders, and active swimmers; in these tadpoles, hind limb and forelimb-buds emerged on 16th and 24th day and metamorphosis was complete on 32nd day. Eggs exposed to acephate also hatched on 3rd day but larvae exhibited deformities such as, (i) tail distortions, (ii) laterally crooked trunk, (iii) decreased pigmentation, (iv) inactivity, (v) peeling of the skin, and (vi) delay in emergence of limbs and completion of metamorphosis. Cypermethrin-exposed eggs exhibited a delay (4-8 days) in hatching, there was no mortality, deformities in tail, trunk and head region, delay in the emergence of limbs, and completion of metamorphosis were evident. The demonstrated data indicate that these pesticides interfere with amphibian development when present in the aquatic system.

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“…In an experiment with fathead minnows exposed to Aroclor® 1242 (Monsanto, St. Louis, MO, USA) a commercial PCB mixture, eggs maintained at high concentrations (15 to 51 μg/L) hatched with good success, but none of the fry survived [17]. Dial and Bauer [34] found leopard frog eggs to be very resistant to paraquat, a herbicide, until approximately 3 d posthatch, when a significant increase in mortality was observed in all but one treatment group. Berrill et al [35] also found that in experimental exposures of leopard and green frogs to low concentrations of pyrethroid insecticides, “newly hatched tadpoles are considerably more sensitive than embryo stages.”…”

Section: Discussionmentioning

confidence: 99%

Effects of polychlorinated biphenyl 126 on green frog (Rana clamitans) and leopard frog (Rana pipiens) hatching success, development, and metamorphosis

Rosenshield

Jofré

Karasov

1999

Enviro Toxic and Chemistry

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Abstract-Although increasing evidence links planar chlorinated hydrocarbons, such as polychlorinated biphenyls (PCBs), to decreases in survival and reproduction of fish, mammals, and birds near Green Bay, Wisconsin, and the Great Lakes, USA, relatively little is known of their bioaccumulation or of their possible effects in amphibians. We exposed embryos and larvae of two ranid species commonly occurring in the Green Bay ecosystem, the green frog (Rana clamitans) and the leopard frog (Rana pipiens), to PCB 126, a model coplanar PCB compound. Nominal concentrations ranged from 0.005 to 50 g/L, and exposure lasted through metamorphosis. Tissue concentrations of PCB 126 in tadpoles that did not metamorphose by the end of the experiment ranged from 1.2 to 9,600 ng/g wet mass. No significant mortality of embryos occurred before hatching; however, survival of larvae was significantly reduced at the highest concentration for both species. Few deformities were observed, but the incidence of edema was significantly higher in tadpoles exposed to 50 g/L. Swimming speed and growth of tadpoles was also significantly reduced in this treatment. The percent of tadpoles that reached metamorphosis was significantly lower in green frogs at the highest concentration, and no leopard frogs survived past day 47 of the experiment in this treatment. At high concentrations, PCB 126 affected both ranid species; however, sublethal effects were not apparent for the parameters we measured at concentrations that occur in water in the Green Bay ecosystem.

show abstract

Teratogenic and lethal effects of paraquat on developing frog embryos (Rana pipiens)

Cited by 30 publications

References 4 publications

Effects of Polychlorinated Biphenyl 126 on Green Frog(rana Clamitans) and Leopard Frog (Rana Pipiens)hatching Success, Development, and Metamorphosis

Effects of Polychlorinated Biphenyl 126 on Green Frog(rana Clamitans) and Leopard Frog (Rana Pipiens)hatching Success, Development, and Metamorphosis

Morphological and behavioral alterations induced by endocrine disrupters in amphibian tadpoles

Effects of polychlorinated biphenyl 126 on green frog (Rana clamitans) and leopard frog (Rana pipiens) hatching success, development, and metamorphosis

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