Tension‐voltage relationship in membrane fusion and its implication in exocytosis

Ramos, Corinne; Teissié, Justin

doi:10.1016/s0014-5793(99)01739-1

Cited by 11 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the vesicles were purely lipidic, there was no contribution of a protein scaffold in the present study. We previously showed that mechanical constraints play a positive role in electrofusion [23]. This strongly suggests that, within the present description, exocytosis will be facilitated by the mechanical strain in the contact area due to the SNARE proteins [24].…”

Section: Discussionsupporting

confidence: 69%

Spontaneous lipid vesicle fusion with electropermeabilized cells

et al. 2002

Self Cite

View full text Add to dashboard Cite

Fusion is obtained between electropermeabilized mammalian cells and intact large unilamellar lipid vesicles. This is monitored by a fluorescence assay. Prepulse contact is obtained by Ca 2+ when negatively charged lipids are present in the liposomes. The mixing of the liposome content in the cell cytoplasm is observed under conditions preserving cell viability. Electric conditions are such that free liposomes are not affected by the external field. Therefore destabilization of only one of the two membranes of the partners is sufficient for fusion. The comparison between the efficiency of dye delivery for different liposome preparations (multilamellar vesicles, large unilamellar vesicles, small unilamellar vesicles) is indicative that more metastable liposomes are more fusable with electropulsated cells. This observation is discussed within the framework of the recent hypothesis that occurrence of a contact induced electrostatic destabilization of the plasma membrane is a key step in the exocytosis process. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

show abstract

Section: Discussionsupporting

confidence: 69%

Spontaneous lipid vesicle fusion with electropermeabilized cells

et al. 2002

Self Cite

View full text Add to dashboard Cite

show abstract

“…Besides electric field parameters, mechanical forces can increase the fusion yield as they enable good contact of cells (Jaroszeski et al 1994;Ramos and Teissié 2000b).…”

Section: Electrofusionmentioning

confidence: 99%

Electroporation in Biological Cell and Tissue: An Overview

Kandušer

Miklavčič²

2008

Electrotechnologies for Extraction From Food Plants and Biomaterials

View full text Add to dashboard Cite

In this chapter, basics and mechanisms of electroporation are presented. Most important electric pulse parameters for electroporation efficiency for different applications that involve introduction of small molecules and macromolecules into the cell or cell membrane electrofusion are described. In all these applications, cell viability has to be preserved. However, in some biotechnological applications, such as liquid food sterilization or water treatment, electroporation is used as a method for efficient cell killing. For all the applications mentioned above, besides electric pulse parameters, other factors, such as electroporation medium composition and osmotic pressure, play significant roles in electroporation effectiveness. For controlled use of the method in all applications, the basic mechanisms of electroporation need to be known. The phenomenon was studied from the single-cell level and dense cell suspension that represents a simplified homogenous tissue model, to complex biological tissues. In the latter, different cell types and electric conductivity that change during the course of electric pulse application can significantly affect the effectiveness of the treatment. For such a complex situation, the design and use of suitable electrodes and theoretical modeling of electric field distribution within the tissue are essential. Electroporation as a universal method applicable to different cell types is used for different purposes. In medicine it is used for electrochemotherapy and genetherapy. In biotechnology it is used for water and liquid food sterilization and for transfection of bacteria, yeast, plant protoplast, and intact plant tissue. Understanding the phenomenon of electroporation, its mechanisms and optimization of all the parameters that affect electroporation is a prerequisite for successful treatment. In addition to the parameters mentioned above, different biological characteristics of treated cell affect the outcome of the treatment. Electroporation, gene electrotransfer and electrofusion are affected by cell membrane fluidity, cytoskeleton, and the presence of the cell wall in bacteria yeast and plant cells. Thus, electroporation parameters need to be specifically optimized for different cell types.

show abstract

“…Electropermeabilization of biological membranes is widely utilized to transfer nucleic acids and other macromolecules through membranes of different eukaryotic and prokaryotic cells (Neumann et al, 1982;Rols and Teissie, 1998). High electric fields are also used to fuse cells (Neil and Zimmermann, 1993;Ramos and Teissie, 2000). We hope that further development of these biomedical and biotechnological applications will eventually benefit from better understanding of the physical mechanisms underlying voltage-induced formation and evolution of the pores.…”

Section: Lipidic Pores and Biologically Relevant Processesmentioning

confidence: 99%

Voltage-Induced Nonconductive Pre-Pores and Metastable Single Pores in Unmodified Planar Lipid Bilayer

Melikov

Frolov

Щербаков

et al. 2001

Biophysical Journal

241

192

View full text Add to dashboard Cite

Electric fields promote pore formation in both biological and model membranes. We clamped unmodified planar bilayers at 150-550 mV to monitor transient single pores for a long period of time. We observed fast transitions between different conductance levels reflecting opening and closing of metastable lipid pores. Although mean lifetime of the pores was 3 +/- 0.8 ms (250 mV), some pores remained open for up to approximately 1 s. The mean amplitude of conductance fluctuations (approximately 500 pS) was independent of voltage and close for bilayers of different area (40,000 and 10 microm(2)), indicating the local nature of the conductive defects. The distribution of pore conductance was rather broad (dispersion of approximately 250 pS). Based on the conductance value and its dependence of the ion size, the radius of the average pore was estimated as approximately 1 nm. Short bursts of conductance spikes (opening and closing of pores) were often separated by periods of background conductance. Within the same burst the conductance between spikes was indistinguishable from the background. The mean time interval between spikes in the burst was much smaller than that between adjacent bursts. These data indicate that opening and closing of lipidic pores proceed through some electrically invisible (silent) pre-pores. Similar pre-pore defects and metastable conductive pores might be involved in remodeling of cell membranes in different biologically relevant processes.

show abstract

Tension‐voltage relationship in membrane fusion and its implication in exocytosis

Cited by 11 publications

References 33 publications

Spontaneous lipid vesicle fusion with electropermeabilized cells

Spontaneous lipid vesicle fusion with electropermeabilized cells

Electroporation in Biological Cell and Tissue: An Overview

Voltage-Induced Nonconductive Pre-Pores and Metastable Single Pores in Unmodified Planar Lipid Bilayer

Contact Info

Product

Resources

About