Tensile strength and histological changes of abdominal aorta of malnourished rats

Yoshida, Winston Bonetti; Müller, Sérgio Swain; Carvalho, Isabela Feitosa de; Fabris, Viciany Erique; Naresse, Luiz Eduardo; Maffei, Fábio

doi:10.1016/0967-2109(95)94165-s

Cited by 11 publications

(9 citation statements)

References 4 publications

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“…The achieved tensile strength of the PUR grafts exceeds the one of natural vessels: Yoshida et al reported an axial tensile strength of 3.1N for the rat aorta when compared with 5.8N of our prosthesis. 28 Experiments performed in our laboratory showed a three times higher fracture force in circumferential direction for the polyurethane prostheses in comparison to specimens of the rat abdominal aorta (unpublished data).…”

Section: Discussionmentioning

confidence: 89%

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Electrospun polyurethane vascular grafts: In vitro mechanical behavior and endothelial adhesion molecule expression

Grasl

Bergmeister

Stoiber

et al. 2009

J Biomedical Materials Res

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Engineered small diameter vascular grafts must closely match mechanical characteristics of native vessels and exhibit stimulus-responsive bioactivity. In this study, mechanical homogeneity of electrospun small diameter polyurethane grafts as well as spontaneous attachment, proliferation, and adhesion molecule expression of endothelial cells (EC) in their presence was studied in vitro. Axial and circumferential tensile strengths were measured and found to be twofold higher in the circumferential direction. EC attachment was easily achieved without precoating the fiber matrix. Stimulation of EC with interleukin-1beta (IL-1beta) led to a statistically significant upregulation of the adhesion molecules E-Selectin, ICAM-1, and VCAM-1. Quantification of adhesion molecule expression by means of energy-dispersive X-ray microanalysis revealed no differences in the stimulatory responses of EC cultured on electrospun polyurethane when compared with cells grown on tissue culture-treated cover slips. Summarizing, highly uniform small diameter polyurethane grafts were fabricated and shown to allow spontaneous EC attachment. The synthetic graft surface neither impaired the endothelial response toward IL-1beta stimulation nor did it adversely affect the regulation of expression of endothelial adhesion molecules.

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Section: Discussionmentioning

confidence: 89%

“…28 Experiments performed in our laboratory showed a three times higher fracture force in circumferential direction for the polyurethane prostheses in comparison to specimens of the rat abdominal aorta (unpublished data).…”

Section: Discussionmentioning

confidence: 92%

Electrospun polyurethane vascular grafts: In vitro mechanical behavior and endothelial adhesion molecule expression

Grasl

Bergmeister

Stoiber

et al. 2009

J Biomedical Materials Res

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“…0.018þF/3700 KN and operates in conjunction with a microcomputer using Mtest s 1.00 software. The velocity chosen for tension application was 30 mm/min [22,23]. At the end of the test, the software provided a load-elongation diagram and the results of yield point (N), stiffness coefficient (N/mm) and failure load (N).…”

Section: Biomechanical Characterizationmentioning

confidence: 99%

Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds

Bertanha

Moroz

Jaldin

et al. 2014

Experimental Cell Research

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Clinical experience for peripheral arterial disease treatment shows poor results when synthetic grafts are used to approach infrapopliteal arterial segments. However, tissue engineering may be an option to yield surrogate biocompatible neovessels. Thus, biological decellularized scaffolds could provide natural tissue architecture to use in tissue engineering, when the absence of ideal autologous veins reduces surgical options. The goal of this study was to evaluate different chemical induced decellularization protocols of the inferior vena cava of rabbits. They were decellularized with Triton X100 (TX100), sodium dodecyl sulfate (SDS) or sodium deoxycholate (DS). Afterwards, we assessed the remaining extracellular matrix (ECM) integrity, residual toxicity and the biomechanical resistance of the scaffolds. Our results showed that TX100 was not effective to remove the cells, while protocols using SDS 1% for 2h and DS 2% for 1h, efficiently removed the cells and were better characterized. These scaffolds preserved the original organization of ECM. In addition, the residual toxicity assessment did not reveal statistically significant changes while decellularized scaffolds retained the equivalent biomechanical properties when compared with the control. Our results concluded that protocols using SDS and DS were effective at obtaining decellularized scaffolds, which may be useful for blood vessel tissue engineering.

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“…There are a number of mechanisms, reported in both experimental and human studies, by which malnutrition early in life could initiate or further increase higher BP: activation of the renin-angiotensin system [25], alterations in vascular structure, function and compliance [26, 27], increased sympathetic nervous activity [28], increased insulin sensitivity [29]and high plasma glucocorticoid concentrations [30]. …”

Section: Discussionmentioning

confidence: 99%

Malnutrition Is Associated with Increased Blood Pressure in Childhood

Sesso

Barreto²,

Neves³

et al. 2004

Nephron Clin Pract

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Background/Aims: Protein-energy malnutrition remains a major public health problem in many countries. Scanty information is available about the effects of malnutrition during childhood on blood pressure (BP). Methods: In a cross-sectional study we assessed the BP of 172 children older than 2 years living in shantytowns in São Paulo city. Ninety-one children were malnourished (height-for-age or weight-for-age Z-score below –1 of the NCHS references); 20 had recovered from malnutrition after an average time of 6.4 years, and 61 were non-malnourished controls. Results: A greater percentage of children in the malnourished and recovered groups had increased systolic or diastolic BP (>95th percentile of the Update of the 2nd Task Force references) after adjusting for age, sex and height, compared to the controls (29, 20 and 2%, respectively, p < 0.001). Mean diastolic BP, adjusted for age, sex, race, weight, height and birth weight, was significantly increased in malnourished and recovered children compared to controls (65.2 ± 0.6, 66.5 ± 1.5, and 61.8 ± 0.8 mm Hg, respectively, p < 0.01). Conclusions: BP is increased in malnourished children and in those who recovered from malnutrition after an average period of 6 years. Malnutrition occurring during childhood may represent a risk factor for increased BP later in life.

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Tensile strength and histological changes of abdominal aorta of malnourished rats

Cited by 11 publications

References 4 publications

Electrospun polyurethane vascular grafts: In vitro mechanical behavior and endothelial adhesion molecule expression

Electrospun polyurethane vascular grafts: In vitro mechanical behavior and endothelial adhesion molecule expression

Morphofunctional characterization of decellularized vena cava as tissue engineering scaffolds

Malnutrition Is Associated with Increased Blood Pressure in Childhood

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