Tenectin is a novel αPS2βPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila

Fraichard, Stéphane; Bougé, Anne-Laure; Kendall, Timmy; Chauvel, Isabelle; Bouhin, Hervé; Bunch, Thomas A.

doi:10.1016/j.ydbio.2010.02.008

Cited by 19 publications

(28 citation statements)

References 108 publications

(132 reference statements)

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“…As mentioned above, tnc encodes an extracellular matrix protein involved in cell adhesion. Consistent with a role for tnc in mediating adhesion between the dorsal and ventral surfaces of the wing pouch, RNAimediated knockdown of tnc results in defects in wing morphology (Fraichard et al 2010). We cloned two temporally dynamic open chromatin sites located ∼40 kb upstream of the tnc promoter.…”

Section: Temporally Dynamic Open Chromatin Sites Correspond To Tempormentioning

confidence: 78%

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Hormone-dependent control of developmental timing through regulation of chromatin accessibility

Uyehara

Nystrom²,

Niederhuber³

et al. 2017

Genes Dev.

138

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Specification of tissue identity during development requires precise coordination of gene expression in both space and time. Spatially, master regulatory transcription factors are required to control tissue-specific gene expression programs. However, the mechanisms controlling how tissue-specific gene expression changes over time are less well understood. Here, we show that hormone-induced transcription factors control temporal gene expression by regulating the accessibility of DNA regulatory elements. Using the Drosophila wing, we demonstrate that temporal changes in gene expression are accompanied by genome-wide changes in chromatin accessibility at temporal-specific enhancers. We also uncover a temporal cascade of transcription factors following a pulse of the steroid hormone ecdysone such that different times in wing development can be defined by distinct combinations of hormone-induced transcription factors. Finally, we show that the ecdysone-induced transcription factor E93 controls temporal identity by directly regulating chromatin accessibility across the genome. Notably, we found that E93 controls enhancer activity through three different modalities, including promoting accessibility of late-acting enhancers and decreasing accessibility of early-acting enhancers. Together, this work supports a model in which an extrinsic signal triggers an intrinsic transcription factor cascade that drives development forward in time through regulation of chromatin accessibility.

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Section: Temporally Dynamic Open Chromatin Sites Correspond To Tempormentioning

confidence: 78%

“…S2A). For example, the tenectin gene (tnc), which encodes a constituent of the extracellular matrix that binds α-PS2 integrin (Fraichard et al 2010), exhibits multiple open chromatin changes between L3, 24-h, and 44-h wings ( Fig. 2A).…”

Section: Open Chromatin Profiles Are Temporally Dynamic In Pupal Wingsmentioning

confidence: 99%

Hormone-dependent control of developmental timing through regulation of chromatin accessibility

Uyehara

Nystrom²,

Niederhuber³

et al. 2017

Genes Dev.

138

View full text Add to dashboard Cite

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“…It encodes an integrin binding protein related to developmental processes and is regulated by ecdysone during wing development. Transcripts for this gene were recently identified in Drosophila leg discs, eye and brain ( Fraichard et al , 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis

Santos

Hartfelder

2015

Genet. Mol. Biol.

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Phenotypic plasticity is a hallmark of the caste systems of social insects, expressed in their life history and morphological traits. These are best studied in bees. In their co-evolution with angiosperm plants, the females of corbiculate bees have acquired a specialized structure on their hind legs for collecting pollen. In the highly eusocial bees (Apini and Meliponini), this structure is however only present in workers and absent in queens. By means of histological sections and cell proliferation analysis we followed the developmental dynamics of the hind legs of queens and workers in the fourth and fifth larval instars. In parallel, we generated subtractive cDNA libraries for hind leg discs of queen and worker larvae by means of a Representational Difference Analysis (RDA). From the total of 135 unique sequences we selected 19 for RT-qPCR analysis, where six of these were confirmed as differing significantly in their expression between the two castes in the larval spinning stage. The development of complex structures such as the bees’ hind legs, requires diverse patterning mechanisms and signaling modules, as indicated by the set of differentially expressed genes related with cell adhesion and signaling pathways.

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“…The fact that rhea/talin and mys are involved in integrin adhesion suggests an important role of cell-extracellular matrix (ECM) adhesion for genitalia LR asymmetry. Of note, mutations in the Drosophila tenectin gene, encoding a ligand for PS2 integrin, can induce genitalia rotation defects [32], and recent work showed that genitalia rotation involves cell intercalation and asymmetry of junction remodeling [31]. A role for cell adhesion and ECM has also been shown in vertebrates for LR asymmetry.…”

Section: Actin Nucleation Cell Polarity and Cell Adhesion Are Importmentioning

confidence: 99%

The Drosophila actin nucleator DAAM is essential for left-right asymmetry

et al. 2020

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Left-Right (LR) asymmetry is essential for organ positioning, shape and function. Myosin 1D (Myo1D) has emerged as an evolutionary conserved chirality determinant in both Drosophila and vertebrates. However, the molecular interplay between Myo1D and the actin cytoskeleton underlying symmetry breaking remains poorly understood. To address this question, we performed a dual genetic screen to identify new cytoskeletal factors involved in LR asymmetry. We identified the conserved actin nucleator DAAM as an essential factor required for both dextral and sinistral development. In the absence of DAAM, organs lose their LR asymmetry, while its overexpression enhances Myo1D-induced de novo LR asymmetry. These results show that DAAM is a limiting, LR-specific actin nucleator connecting up Myo1D with a dedicated F-actin network important for symmetry breaking.

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Tenectin is a novel αPS2βPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila

Cited by 19 publications

References 108 publications

Hormone-dependent control of developmental timing through regulation of chromatin accessibility

Hormone-dependent control of developmental timing through regulation of chromatin accessibility

Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis

The Drosophila actin nucleator DAAM is essential for left-right asymmetry

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