Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis

Santos, Carolina G.; Hartfelder, Klaus

doi:10.1590/s1415-475738320140393

Cited by 7 publications

(95 citation statements)

References 69 publications

(95 reference statements)

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“…For suppression subtractive hybridization, we used a RDA protocol (Pastorian et al ., ) adapted for social Hymenoptera (Judice et al ., ; Santos & Hartfelder, ). We subtractively hybridized an accessory gland library prepared from cDNA from winged male MAGs (tester) against that of the MAGs from ergatoid males (driver) and vice versa.…”

Section: Methodsmentioning

confidence: 99%

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Accessory gland proteins of males in the male-diphenic antCardiocondyla obscurior

et al. 2018

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Seminal fluid proteins are important agents in sperm competition and sexual conflict. In solitary insects, males and females may have different interests concerning the outcome of a mating episode, and males may use seminal fluid to manipulate the physiology and behaviour of the female against the latter's fitness interests. Less is known about the role of seminal fluids in social insects, where the interests of mating partners are more aligned because of life‐long pair‐bonding. In the male‐diphenic ant Cardiocondyla obscurior, mating even with sterilized males appears to prolong the lifespan of queens, suggesting a positive role of seminal fluids. In the present study, using two approaches (two‐dimensional gel electrophoresis/mass spectrometry and representational difference analysis), we analyze the seminal fluid and the male accessory glands of both winged and wingless males of C. obscurior. The results obtained reveal the presence of hundreds of proteins, of which proteases and protease inhibitors are also widespread in the seminal fluids of other insects.

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Section: Methodsmentioning

confidence: 99%

“…For suppression subtractive hybridization, we used a RDA protocol (Pastorian et al, 2000) adapted for social Hymenoptera (Judice et al, 2006;Santos & Hartfelder, 2015).…”

Section: Representational Difference Analysis (Rda)mentioning

confidence: 99%

Accessory gland proteins of males in the male-diphenic antCardiocondyla obscurior

et al. 2018

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“…Although the gene 14-3-3 epsilon is linked to several functions in flies (e.g. embryonic hatching, germ cell migration, gonad formation, wing venation and eye development, according to Flybase), this gene was found to be consistently expressed in queens and workers of honeybees (Santos & Hartfelder, 2015) providing evidence for its suitability for gene normalization.…”

Section: Short Notementioning

confidence: 99%

A Scientific Note of Housekeeping Genes for the Primitively Eusocial bee Euglossa viridissima Friese (Apidae: Euglossini)

Boff

Friedel²,

Miertsch

et al. 2018

Sociobiology

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Studies on the expression of genes in different contexts are essential to our understanding of the functioning of organisms and their adaptations to the environment. Gene expression studies require steps of normalization, which are done using the stable expression pattern of reference genes. For many different eusocial bees reference genes have been discovered, but not for the primitively eusocial euglossine bees.We used available genomic resources of euglossine species and the gene information of Apis melliferato develop a set of reference genes for the primitive eusocial bee Euglossaviridissima. We tested nine genes in distinct developmental stages three different algorithms to infer the stability of gene expression. The Tata binding protein(Tbp) and 14-3-3epsilon were the most stable genes across all different stages. The strongest deviation in gene expression pattern occurred in pupae, which require a different set of genes for normalizing gene expression.

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“…Insights into the dynamics of hind leg development in honey bee ( Apis mellifera L.) queen and worker larvae—a morphology/differential gene expression analysis (Santos and Hartfelder, )…”

Section: Latin America: a Living Laboratory For Evo‐devo Researchmentioning

confidence: 99%

Evolutionary Developmental Biology (Evo-Devo) Research in Latin America

Marcellini

González

Sarrazin

et al. 2016

J. Exp. Zool. (Mol. Dev. Evol.)

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Famous for its blind cavefish and Darwin's finches, Latin America is home to some of the richest biodiversity hotspots of our planet. The Latin American fauna and flora inspired and captivated naturalists from the nineteenth and twentieth centuries, including such notable pioneers such as Fritz Müller, Florentino Ameghino, and Léon Croizat who made a significant contribution to the study of embryology and evolutionary thinking. But, what are the historical and present contributions of the Latin American scientific community to Evo-Devo? Here, we provide the first comprehensive overview of the Evo-Devo laboratories based in Latin America and describe current lines of research based on endemic species, focusing on body plans and patterning, systematics, physiology, computational modeling approaches, ecology, and domestication. Literature searches reveal that Evo-Devo in Latin America is still in its early days; while showing encouraging indicators of productivity, it has not stabilized yet, because it relies on few and sparsely distributed laboratories. Coping with the rapid changes in national scientific policies and contributing to solve social and health issues specific to each region are among the main challenges faced by Latin American researchers. The 2015 inaugural meeting of the Pan-American Society for Evolutionary Developmental Biology played a pivotal role in bringing together Latin American researchers eager to initiate and consolidate regional and worldwide collaborative networks. Such networks will undoubtedly advance research on the extremely high genetic and phenotypic biodiversity of Latin America, bound to be an almost infinite source of amazement and fascinating findings for the Evo-Devo community.

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Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis

Cited by 7 publications

References 69 publications

Accessory gland proteins of males in the male-diphenic antCardiocondyla obscurior

Accessory gland proteins of males in the male-diphenic antCardiocondyla obscurior

A Scientific Note of Housekeeping Genes for the Primitively Eusocial bee Euglossa viridissima Friese (Apidae: Euglossini)

Evolutionary Developmental Biology (Evo-Devo) Research in Latin America

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