Tenectin, a novel extracellular matrix protein expressed during Drosophila melanogaster embryonic development

Fraichard, Stéphane; Bougé, Anne-Laure; Chauvel, Isabelle; Bouhin, Hervé

doi:10.1016/j.modgep.2006.01.007

Cited by 14 publications

(22 citation statements)

References 9 publications

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“…2B) [27,29]. In tnc mutants, the hindgut tube diameter remains narrow, while overexpression of Tnc leads to diametric overexpansion in a dose-dependent fashion (Fig.…”

Section: A Non-chitinous Matrix Drives Diameter Expansion Of the Drosmentioning

confidence: 93%

Luminal matrices: An inside view on organ morphogenesis

Luschnig

2014

Experimental Cell Research

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Tubular epithelia come in various shapes and sizes to accommodate the specific needs for transport, excretion and absorption in multicellular organisms. The intestinal tract, glandular organs and conduits for liquids and gases are all lined by a continuous layer of epithelial cells, which form the boundary of the luminal space. Defects in epithelial architecture and lumen dimensions will impair transport and can lead to serious organ malfunctions. Not surprisingly, multiple cellular and molecular mechanisms participate in controlling size and form of tubular epithelial structures. One intriguing aspect of epithelial organ formation is the coordinate behavior of individual cells as they mold the mature lumen. Here, we focus on recent findings, primarily from Drosophila, demonstrating that informative cues can emanate from the developing organ lumen in the form of solid luminal material. The luminal material is produced by the surrounding epithelium and helps to coordinate changes in shape and arrangement of the very same cells, resulting in correct lumen dimensions.

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“…2B) [27,29]. In tnc mutants, the hindgut tube diameter remains narrow, while overexpression of Tnc leads to diametric overexpansion in a dose-dependent fashion (Fig.…”

Section: A Non-chitinous Matrix Drives Diameter Expansion Of the Drosmentioning

confidence: 93%

Luminal matrices: An inside view on organ morphogenesis

Luschnig

2014

Experimental Cell Research

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“…After washing in PBS, the imaginal discs and the larval brains were incubated overnight at 4–8°C with primary antibodies diluted in PBS containing 5% normal goat serum, 0.1% Triton X-100, and 0.02% sodium azide. Primary antibodies were mouse anti-elav (1:1000; Developmental Studies Hybridoma Center, University Iowa) and anti-tenectin (1:4000; Fraichard et al, 2006). Detection of the different primary antibodies was carried out using alkaline phosphatase anti-rabbit (1:50, Sigma), AlexaFluor594 anti-mouse (1:200, Molecular Probes), and AlexaFluor488 anti-rabbit (1:50, Molecular Probes).…”

Section: Methodsmentioning

confidence: 99%

“…To analyze the role of tenebrin in development, we identified its homolog, tenectin , in Drosophila melanogaster and described its embryonic expression patterns (Fraichard et al, 2006). In this report we used tenectin dsRNA to generate tenectin mutants and find phenotypes in the adult wing and male genitalia.…”

Section: Introductionmentioning

confidence: 99%

Tenectin is a novel αPS2βPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila

Fraichard

Bougé

Kendall

et al. 2010

Developmental Biology

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Morphogenesis of the adult structures of holometabolous insects is regulated by ecdysteroids and juvenile hormones and involves cell-cell interactions mediated in part by the cell surface integrin receptors and their extracellular matrix (ECM) ligands. These adhesion molecules and their regulation by hormones are not well characterized. We describe the gene structure of a newly described ECM molecule, tenectin, and demonstrate that it is a hormonally regulated ECM protein required for proper morphogenesis of the adult wing and male genitalia. Tenectin’s function as a new ligand of the PS2 integrins is demonstrated by both genetic interactions in the fly and by cell spreading and cell adhesion assays in cultured cells. Its interaction with the PS2 integrins is dependent on RGD and RGD-like motifs. Tenectin’s function in looping morphogenesis in the development of the male genitalia led to experiments that demonstrate a role for PS integrins in the execution of left-right asymmetry.

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“…These molecules include Thrombospondin (Tsp) (Adams et al, 2003), M-spondin (Mspo; a homologue of F-spondin/VSGP/Spon1) (Umemiya et al, 1997), Papilin (Ppn; an alternatively spliced, conserved, prominent constituent of BMs with thrombospondin type-1 domains and homologies to the carboxyterminal of ADAMTS group metalloproteases) (Campbell et al, 1987; Kramerova et al, 2003; Fessler et al, 2004), Multiplexin (Mp; a homologue of collagens XV and XVIII with an endostatin domain) (Meyer and Moussian, 2009; Momota et al, 2011), Faulty attraction (Frac; a protein rich in EGF-like and calcium-binding EGF domains, related to the Fibrillin and Fibulin protein families) (Miller et al, 2011), Tiggrin (Tig; containing unique N- and C-terminal domains and a central stretch of sixteen contiguous ~75 amino acid repeats, essentially modified through mucin-type O -glycosylation) (Fogerty et al, 1994; Bunch et al, 1998; Zhang et al, 2011), Tenectin (Tnc; containing a signal peptide, a RGD tripeptide, and a c-type von Willebrand Factor domain) (Fraichard et al, 2006), Glutactin (Glt; containing a catalytically inactive acetylcholine esterase domain) (Olson et al, 1990), Peroxidasin (Pxn, containing leucin rich and immuno globulin repeats, and a functional peroxidase domain) (Nelson et al, 1994), and MDP-1 (Macrophage-derived proteoglycan-1; potentially synonymous to Papilin) (Hortsch et al, 1998). Indications of a potential Drosophila Fibronectin (Gratecos et al, 1988) have not been confirmed by subsequent studies or genome annotations.…”

Section: Ecm Molecules In Drosophilamentioning

confidence: 99%

Extracellular matrix and its receptors indrosophilaneural development

Broadie

Baumgärtner

Prokop

2011

Developmental Neurobiology

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Extracellular matrix (ECM) and matrix receptors are intimately involved in most biological processes. The ECM plays fundamental developmental and physiological roles in health and disease, including processes underlying the development, maintenance and regeneration of the nervous system. To understand the principles of ECM-mediated functions in the nervous system, genetic model organisms like Drosophila provide simple, malleable and powerful experimental platforms. This article provides an overview of ECM proteins and receptors in Drosophila. It then focuses on their roles during three progressive phases of neural development: 1) neural progenitor proliferation, 2) axonal growth and pathfinding and 3) synapse formation and function. Each section highlights known ECM and ECM-receptor components and recent studies done in mutant conditions to reveal their in vivo functions, all illustrating the enormous opportunities provided when merging work on the nervous system with systematic research into ECM-related gene functions.

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Tenectin, a novel extracellular matrix protein expressed during Drosophila melanogaster embryonic development

Cited by 14 publications

References 9 publications

Luminal matrices: An inside view on organ morphogenesis

Luminal matrices: An inside view on organ morphogenesis

Tenectin is a novel αPS2βPS integrin ligand required for wing morphogenesis and male genital looping in Drosophila

Extracellular matrix and its receptors indrosophilaneural development

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