Ten new polymorphic microsatellite loci for North American river otters (<i>Lontra canadensis</i>) and their utility in related mustelids

Beheler, Amanda S.; Fike, Jennifer A.; Dharmarajan, Guha; Rhodes, Olin E.; Serfass, Thomas L.

doi:10.1111/j.1471-8286.2005.01005.x

Cited by 36 publications

(26 citation statements)

References 10 publications

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“…Subsequently because of the loss of released individuals, the occurrence of offspring and the increasing relatedness among individuals, we had to gradually increase the number of microsatellite loci: OT04, OT05, OT07, OT14, OT17, OT19 and OT22 (Huang et al 2005) and RI18 (Beheler et al 2005). We only used tetranucleotide microsatellite loci to reduce the occurrence of stutter bands and ambiguity in scoring that often happens with dinucleotide loci.…”

Section: Genetic Typingmentioning

confidence: 99%

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

et al. 2010

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Section: Genetic Typingmentioning

confidence: 99%

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

et al. 2010

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“…Thirteen microsatellite loci identified in the Eurasian otter, Lutra lutra, [Lut435, Lut453, Lut604, Lut701, Lut733 and Lut782 (Dallas and Piertney 1998); 04OT17 (Huang et al 2005)] and North American river otter, Lontra canadensis, [RIO3 and RIO6 (Beheler et al 2004); RIO12, RIO15, RIO16 and RIO17 (Beheler et al 2005)] were used in this study. These markers had previously been PCR amplified in the giant otter and were found to be polymorphic (Pickles et al 2009).…”

Section: Dna Extraction and Amplificationmentioning

confidence: 99%

Genetic diversity and population structure in the endangered giant otter, Pteronura brasiliensis

et al. 2011

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We assessed levels of genetic diversity and investigated patterns of population structure in three remnant populations of the endangered giant otter, Pteronura brasiliensis, using microsatellite loci. All populations displayed moderate to low levels of heterozygosity and allelic richness (H O 0.56-0.57, A R 4.00-5.15) and effective population sizes were low (N E 10.8-54) although only the Iténez population exhibited the signature of a genetic bottleneck. Population structure analyses revealed a pattern in which the populations of the Upper Amazon, Orinoco and Essequibo drainages comprised partially differentiated segments of a northern South American metapopulation, whereas the population of the Iténez appeared isolated. The observed patterns are congruent with previous mitochondrial DNA analysis which suggested the Iténez and northern South American groups constitute two evolutionary significant units. The results presented here should be considered in planning future policies aiming to manage the recovery of the giant otter across its range.

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“…Briefly, we extracted DNA from river otter blood, feces, and hair follicles, and amplified the DNA with PCR using standard protocols. We scored eight microsatellite loci to distinguish among individual otters, including three tetranucleotide (Rio01, Rio17, and Rio19) and one trinucleotide (Rio05) markers developed for river otters (Beheler et al, 2004(Beheler et al, , 2005, and four tetranucleotide markers (Lut701, Lut733, Lut801, and Lut829) developed for Eurasian otters (Lutra lutra; Dallas and Piertney, 1998). In addition, we used the LutSRY locus for sex identification (Dallas et al, 2000).…”

Section: Dna Analysismentioning

confidence: 99%

Indirect effects of bioinvasions in Yellowstone Lake: The response of river otters to declines in native cutthroat trout

Crait

Regehr

Ben‐David

2015

Biological Conservation

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Nonnative species threaten ecosystems throughout the world -including protected reserves. In Yellowstone National Park, river otters Lontra canadensis depend on native cutthroat trout as prey. However, nonnative lake trout and whirling disease have significantly reduced the abundance of these native fish in the park's largest body of water, Yellowstone Lake. We studied the demographic and behavioral responses of otters to declining cutthroat trout on Yellowstone Lake and its tributaries. From 2002-2008, we monitored otter activity at latrine (scentmarking) sites, collected scat for prey identification, and used individual genotypes from scat and hair samples to evaluate survival and abundance with capture-recapture methods. Otter activity at latrines decreased with declines in cutthroat trout, and the prevalence of these fish in otter scat declined from 73% to 53%. Cutthroat trout numbers were the best predictor of temporal variation in apparent survival, and mean annual survival for otters was low (0.72). The density of otters in our study area (1 otter per 13.4 km of shoreline) was also low, and evidence of a recent genetic bottleneck suggests that otter abundance might have declined prior to our study. River otters in and around Yellowstone Lake appear to be responding to reductions in cutthroat trout via changes in distribution, diet, and possibly survival and abundance. Our results provide a baseline estimate for monitoring the broader outcome of management efforts to conserve native cutthroat trout and emphasize the indirect ecosystem consequences of invasive species.

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Ten new polymorphic microsatellite loci for North American river otters (Lontra canadensis) and their utility in related mustelids

Cited by 36 publications

References 10 publications

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

The reintroduction of the Eurasian otter (Lutra lutra) into the Netherlands: hidden life revealed by noninvasive genetic monitoring

Genetic diversity and population structure in the endangered giant otter, Pteronura brasiliensis

Indirect effects of bioinvasions in Yellowstone Lake: The response of river otters to declines in native cutthroat trout

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