Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck

Easty, D.M.; Easty, G. C.; Carter, R L; Monaghan, Paul; Butler, L. J.

doi:10.1038/bjc.1981.115

Cited by 170 publications

(81 citation statements)

References 24 publications

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“…There was also no correlation with anaplasia, the cell line with the highest levels, LICR-LON-HN-5 having been demonstrated to keratinise in culture with spinous cells, keratohyaline granule containing cells and the formation of a cornified envelope (Monaghan et al, 1983;Knight et al, 1984) while the line LICR-LON-HN-2 with ten-fold fewer receptors is relatively poorly differentiated both in vitro and in vivo (Easty et al, 1981a;Cowley et al, 1984).…”

Section: Discussionmentioning

confidence: 99%

Increased EGF receptors on human squamous carcinoma cell lines

Cowley¹,

Smith²,

Gusterson³

1986

Br J Cancer

116

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Section: Discussionmentioning

confidence: 99%

Increased EGF receptors on human squamous carcinoma cell lines

Cowley¹,

Smith²,

Gusterson³

1986

Br J Cancer

116

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“…Human cells from a number of lineages including keratinocytes are known to exhibit a limited proliferative capacity in vitro (Hay¯ick, 1965;Rheinwald and Green, 1975) but in contrast, many human tumors, including squamous cell carcinoma of the head and neck (SCC-HN), contain variant cells which proliferate essentially inde®nitely and are designated immortal (Rheinwald and Beckett, 1981;Easty et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Evidence for the inactivation of multiple replicative lifespan genes in immortal human squamous cell carcinoma keratinocytes

Loughran

Bond

et al. 1997

Oncogene

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Human keratinocyte immortality is genetically recessive to the normal phenotype of limited replicative lifespan and appears to require the dysfunction of p53 and the cyclin D-Cdk inhibitor p16. In order to test for the inactivation of other candidate replicative lifespan genes in the immortal cells of human tumors, we developed a series of mortal and immortal keratinocyte cultures derived from neoplastic lesions of the head and neck which were amenable to molecular genetic analysis by the loss of heterozygosity (LOH) technique. The results indicate that keratinocyte immortalization in head and neck squamous cell carcinoma (SCC-HN) development involves the inactivation of at least two further pathways to senescence and four in all. Chromosomes 1, 4 and 7 carry genes representing immortality complementation groups C, B and D respectively and immortal keratinocytes showed LOH at either 4q32-q34 between D4S1554 and D4S171 (group B) or 7q31 (group D) but never 1q25 (group C). These results tentatively suggest that the genes responsible for the immortality complementation groups encode proteins on the same pathway to senescence. In addition, all of the immortal keratinocyte lines possessed high levels of telomerase activity and a suppressor of telomerase activity has been mapped to the short arm of chromosome 3p. Five out of eight lines showed LOH at 3p21.2-p21.3, a region which may carry a gene capable of suppressing SCC-HN telomerase. However, alternative mechanisms of telomerase reactivation were also suggested by our results. None of the above genetic alterations were seen in seven senescent neoplastic keratinocyte cultures. Other loci harbouring antiproliferative genes implicated in replicative lifespan showed few or no alterations and any alterations seen were additional to those described above.

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“…However, the establishment of an oral cancer cell line is especially difficult from the primary site, and only a few successful attempts have been reported (Easty et al, 1981;Hu et al, 1984;Roa et al, 1985;Sacks et al, 1988;Tatke et al, 1995;Ji et al, 2001). Most previously established cell lines have been reported from lymph node metastatic foci, or transplanted tumors in nude mice.…”

Section: Introductionmentioning

confidence: 99%

Characterization of newly established oral cancer cell lines derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells

Lee

Kim²,

Lee³

et al. 2005

Exp Mol Med

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Since genetic abnormalities of human cancer are greatly geographically dependent, cultural and environmental backgrounds are thought to be closely related to the carcinogenic process. In the present study, eight human cell lines were established by culture from untreated carcinomas of the oral cancer, of which five were from primary oral squamous cell carcinomas (OSC), one from a mucoepidermoid carcinoma (MEC) and one each originating from metastatic OSC and MEC. All the studied tumor lines grew as monolayers, and showed: i) an epithelial origin by the presence of cytokeratin, and ii) tumorigenic potential in nude mice. Western blot analysis revealed i) over expression of EGFR in six of the cell lines ii) decreased expression of Ecadherin in six cell lines compared to normal human oral mucosa. A mutational analysis showed: point mutations of p53 at exon 7, with transversion, and at exon 8, with transition. These well-characterized human YD cell lines should serve as useful tools in the study of the molecular pathogenesis and biological characteristics of head and neck cancer cells, and in the future testing of new therapeutic reagents for oral cancer.

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Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck

Cited by 170 publications

References 24 publications

Increased EGF receptors on human squamous carcinoma cell lines

Increased EGF receptors on human squamous carcinoma cell lines

Evidence for the inactivation of multiple replicative lifespan genes in immortal human squamous cell carcinoma keratinocytes

Characterization of newly established oral cancer cell lines derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells

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