Characterization of newly established oral cancer cell lines derived from six squamous cell carcinoma and two mucoepidermoid carcinoma cells

Abstract

Since genetic abnormalities of human cancer are greatly geographically dependent, cultural and environmental backgrounds are thought to be closely related to the carcinogenic process. In the present study, eight human cell lines were established by culture from untreated carcinomas of the oral cancer, of which five were from primary oral squamous cell carcinomas (OSC), one from a mucoepidermoid carcinoma (MEC) and one each originating from metastatic OSC and MEC. All the studied tumor lines grew as monolayers, and showed: i) an epithelial origin by the presence of cytokeratin, and ii) tumorigenic potential in nude mice. Western blot analysis revealed i) over expression of EGFR in six of the cell lines ii) decreased expression of Ecadherin in six cell lines compared to normal human oral mucosa. A mutational analysis showed: point mutations of p53 at exon 7, with transversion, and at exon 8, with transition. These well-characterized human YD cell lines should serve as useful tools in the study of the molecular pathogenesis and biological characteristics of head and neck cancer cells, and in the future testing of new therapeutic reagents for oral cancer.

Antitumor effect of a transducible fusogenic peptide releasing multiple proapoptotic peptides by caspase-3

“…The measurement of viable cell numbers was carried out using a mitochondrial reduction activity assay (14). Briefly, cells (3 Â 10 3 per well) were grown in 96-well culture plates overnight, at which time the medium was replaced with fresh medium containing 2% FBS.…”

Activation of B cells using Schneider 2 cells expressing CD40 ligand for the enhancement of antigen presentation in vitro

“…MLR is performed for measuring viable T cell using XTT assay as previously described (Lee et al, 2005). Briefly, whole CD3 + T cells or T cell subsets from healthy individuals were plated at 1 × 10 5 T cells/well with 10 5 irradiated (32 Gy) CD40 activated B cells or B cells/well in 100 µl RPMI-1640 supplemented with 10% FBS (Cambrex), 2 mM glutamine (Gibco/BRL, Gaithersburg, MD) in 96-well plates for up to 6 days.…”

Apicidin inhibits cell growth by downregulating IGF-1R in salivary mucoepidermoid carcinoma cells

“…(19). The cells were maintained as monolayers at 37˚C in an atmosphere containing 5% CO 2 /air in RPMI-1640 containing 10% heat-inactivated fetal bovine serum (FBS; Gibco-BRL, Gaithersburg, MD, uSA) and 1% penicillin/streptomycin (Gibco-BRL).…”