Temporary immersion improves in vitro multiplication and acclimatization of Anthurium andreanum Lind.

Martínez‐Estrada, Eduardo; Islas-Luna, Bartolo; Pérez-Sato, Juan Antonio; Bello-Bello, Jericó Jabín

doi:10.1016/j.scienta.2019.01.053

Cited by 51 publications

(33 citation statements)

References 35 publications

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“…In contrast, Arano-Avalos et al [ 5 ] in taro found an increase in photosynthetic pigment content in the TIB compared to the semi-solid medium. Martínez-Estrada et al [ 6 ] reported an increase in chlorophyll accumulation using the ebb-and-flow bioreactor during the in vitro multiplication of A. andreanum . Jova et al [ 24 ] in yam ( Dioscorea alata L. ‘Pacala Duclos’) observed an increase in photosynthetic pigment content using the TIB compared to the constant immersion system and the static liquid system.…”

Section: Discussionmentioning

confidence: 99%

“…Bello-Bello et al [ 11 ] in banana ( Musa AAA) report an increase in closed stomata in different TISs (20 to 79%). Martínez-Estrada et al [ 6 ] in anthurium ( Anthurium andreanum Lind.) report that shoots grown in the liquid medium had the highest percentages of closed stomata (23–29%).…”

Section: Discussionmentioning

confidence: 99%

“…In vitro propagation of C. esculenta through semi-automated bioreactors (SABs) is an alternative for the production of high-quality genetic and phytosanitary clones [ 5 ]. SABs are designed for in vitro multiplication of plants by tissue, cell or organ in liquid culture medium with a programmed temporary immersion (TI) [ 6 ]. The advantage of SABs is that they increase multiplication rates, allow greater availability of culture medium components and reduce production costs [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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“…On the other hand, red light stimulated the most intensive growth of shoots by internode elongation (the number of nodes did not change), which resulted in the poor quality of shoots (Kim et al, 2004). The use of mixed light also contributed to a higher multiplication ratio in Vanilla planifolia (Bello-Bello et al, 2016), Rosa kordesii (Azmi et al, 2014), and Anthurium andreanum cultures (Martinez-Estrada et al, 2016). The beneficial effect of mixed light (red and blue) on the growth and development of microshoots results from its positive effect on the photosynthesis intensity -energy distribution of red and blue spectrum coincides with the chlorophyll and phytochrome absorption maxima (Kim et al, 2004).…”

Section: Organogenesis In Vitromentioning

confidence: 99%

Influence of light conditions on the morphogenetic and biochemical response of selected ornamental plant species under in vitro conditions: a mini-review

Kulus¹,

Wozny²

2020

bta

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In vitro tissue culture technique, especially micropropagation, is attracting increasing attention in the production of ornamental plants. This technique will probably dominate the horticultural market in the near future. Light is one of the several factors affecting the success of in vitro plant tissue cultures. It directly affects the widely understood morphogenetic response of the explant, i.e., the ability of the explant to grow or regenerate, produce roots, etc. Lighting conditions provided during the in vitro stage may also greatly affect the plant vigor after transferring to nonsterile conditions. Moreover, the necessity of providing artificial light significantly contributes to the total cost of maintaining tissue cultures (related to energy consumption and the need to cool down the heat generated by lamps). Light quantity (intensity) and quality (spectral composition) are the two main parameters that determine its influence on in vitro cultures. This impact depends on the species and other accompanying factors. The aim of this mini-review is to summarize information on the influence of light on the morphogenetic and biochemical response of explants of some selected ornamental plant species grown under in vitro conditions.

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“…O uso de fontes de luz com diferentes comprimentos de onda a partir de diodos emissores de luz (LED) tem sido amplamente utilizado na propagação in vitro em diferentes culturas, como em cana-de-açúcar, bananeira e orquídeas, com o objetivo de aumentar a produção e melhorar a qualidade das plântulas (MALUTA et al, 2013, MARTINEZ et al, 2016HUNG et al 2016). Fontes de luz com comprimentos de ondas diferentes podem causar mudanças significativas no desenvolvimento in vitro de plântulas, relacionadas às disposições dos tilacóides nos cloroplastos, que podem modificar a emissão e comprimento de raízes, número de estômatos nas folhas e no teor de clorofila (MALUTA et al, 2013, VIEIRA et al, 2015, MACEDO et al, 2011.…”

Section: Introductionunclassified

Conservação >i<in vitro>/i< de morangueiros sob diferentes espectros de luz

Casarotto¹

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In vitro conservation of strawberries under different light spectra Light emitting diodes (LEDs) are used in many agriculture sectors, especially in vitro cultivation, because it is a more efficient light source alternative. In addition to providing significant energy savings, it can also help in the development of plants. Some techniques of in vitro cultivation have the purpose of conserving plant genetic resources, which are fundamental to enable the establishment of germplasm banks and allow the commercial production of micropropagated plants. In Brazil, this technique is already used for the propagation of plants, however there are still few experiments with the presence of LEDs. The objective of the study was to evaluate the development of strawberry cv. Camarosa conserved in vitro, under the effect of different light spectra. The strawberry explants were kept in test tubes containing 10 ml of MS semi-solid culture medium, plus 30 g of sucrose L-1; 1.8 g L-1 of Phytagel® and 0.1 mg L-1 of IBA (idolbutyric acid), remaining in vitro for 28, 56, 84, 112 and 140 days under the treatments of mixed white LEDs (70% red + 30% blue), red and blue, without subculture interference. At each interval, height, fresh weight, number and length of roots, number of leaves, stem diameter, chlorophyll a and b contents were evaluated. The seedlings obtained were acclimatized for 21 days and evaluated the survival rate of the plants, expressed as percentage, number of leaves, stem diameter and height of plants. The use of red and mixed color LEDs contributed to the development of the seedlings, while the blue light reduced the development of the seedlings. In relation to the photosynthetic pigments, the use of LEDs did not interfere in any treatment under the seedlings. In acclimatization, the seedlings under the effects of red and mixed lights, had a better survival result in up to three months of conservation without subcultures.

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Temporary immersion improves in vitro multiplication and acclimatization of Anthurium andreanum Lind.

Cited by 51 publications

References 35 publications

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Influence of light conditions on the morphogenetic and biochemical response of selected ornamental plant species under in vitro conditions: a mini-review

Conservação >i<in vitro>/i< de morangueiros sob diferentes espectros de luz

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