Temporal synergism by microencapsulation of piperonyl butoxide and α‐cypermethrin overcomes insecticide resistance in crop pests

Bingham, Georgina; Gunning, Robin V.; Gorman, Kevin; Field, L. M.; Moores, G. D.

doi:10.1002/ps.1336

Cited by 29 publications

(27 citation statements)

References 21 publications

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“…Bingham et al (2007) have shown in laboratory and field experiments that the application of a microencapsulated formulation of PBO and a-cypermethrin drastically reduced the resistance of Bemisia tabaci, Helicoverpa armigera, Aphis gossypii and Myzus persicae to a-cypermethrin. Gunning et al (1996) reported that PBO was likely to facilitate pyrethroid penetration through the cuticle.…”

Section: Discussionmentioning

confidence: 98%

Susceptibility of four field populations of the diamondback moth Plutella xylostella L. (Lepidoptera: Yponomeutidae) to six insecticides in the Sydney region, New South Wales, Australia

Eziah¹,

Rose²,

Clift³

et al. 2008

Australian Journal of Entomology

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Concerns about the failure of insecticides to control the diamondback moth (DBM) Plutella xylostella in the Sydney region of New South Wales, Australia, necessitated the current investigation to establish the susceptibility of four field populations of the DBM to six insecticides. These include two each of organophosphates (OPs), and synthetic pyrethroid insecticides as well as two new products with different modes of action, spinosad and indoxacarb, currently recommended for DBM control in the region. Topical application of the insecticides to the third-instar larvae showed high resistance to pyrethroids (permethrin and esfenvalerate) of 35.0-to 490.0-fold. Resistance to the OPs (methamidophos and chlorpyrifos) and indoxacarb ranged from 12.1-to 36.2-fold and from 11.4-to 34.6-fold, respectively. However, the field populations were susceptible to spinosad (resistance factors only twoto threefold compared with the susceptible strain). A 2 h pre-treatment of the esfenvalerate-resistant strain with the synergists piperonyl butoxide and diethyl maleate increased the toxicity of esfenvalerate by 30.0-and 1.9-fold, respectively, suggesting the involvement of esterases and/or monooxygenases as the key mechanism(s) of insecticide resistance with glutathione S-transferases playing a minor role.

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Section: Discussionmentioning

confidence: 98%

Susceptibility of four field populations of the diamondback moth Plutella xylostella L. (Lepidoptera: Yponomeutidae) to six insecticides in the Sydney region, New South Wales, Australia

Eziah¹,

Rose²,

Clift³

et al. 2008

Australian Journal of Entomology

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“…The PermaNet® 3.0 had a polyethylene roof with 2.8 g/kg ± 25% deltamethrin and 4.0 g/kg ± 25% PBO, and sides coated with 2.8 g/kg ± 25% deltamethrin. PBO is a synergist compound that elevates the rate of penetration of the insecticide into the insect cuticle [24] and inhibits the enzymatic ability of the insect to breakdown the insecticide [11]. …”

Section: Methodsmentioning

confidence: 99%

Efficacy of PermaNet® 3.0 and PermaNet® 2.0 nets against laboratory-reared and wild Anopheles gambiae sensu lato populations in northern Tanzania

2017

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BackgroundMosquitoes have developed resistance against pyrethroids, the only class of insecticides approved for use on long-lasting insecticidal nets (LLINs). The present study sought to evaluate the efficacy of the pyrethroid synergist PermaNet® 3.0 LLIN versus the pyrethroid-only PermaNet® 2.0 LLIN, in an East African hut design in Lower Moshi, northern Tanzania. In this setting, resistance to pyrethroid insecticides has been identified in Anopheles gambiae mosquitoes.MethodsStandard World Health Organization bioefficacy evaluations were conducted in both laboratory and experimental huts. Experimental hut evaluations were conducted in an area where there was presence of a population of highly pyrethroid-resistant An. arabiensis mosquitoes. All nets used were subjected to cone bioassays and then to experimental hut trials. Mosquito mortality, blood-feeding inhibition and personal protection rate were compared between untreated nets, unwashed LLINs and LLINs that were washed 20 times.ResultsBoth washed and unwashed PermaNet® 2.0 and PermaNet® 3.0 LLINs had knockdown and mortality rates of 100% against a susceptible strain of An. gambiae sensu stricto. The adjusted mortality rate of the wild mosquito population after use of the unwashed PermaNet® 3.0 and PermaNet® 2.0 nets was found to be higher than after use of the washed PermaNet® 2.0 and PermaNet® 3.0 nets.ConclusionsGiven the increasing incidence of pyrethroid resistance in An. gambiae mosquitoes in Tanzania, we recommend that consideration is given to its distribution in areas with pyrethroid-resistant malaria vectors within the framework of a national insecticide-resistance management plan.Electronic supplementary materialThe online version of this article (doi:10.1186/s40249-016-0220-z) contains supplementary material, which is available to authorized users.

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“…This is because H. armigera esterases are also inhibited by PBO. [59][60][61][62][63][64] However another study on a different species only saw this effect at high concentrations of PBO (3 mM) of dubious physiological relevance, whereas DEF at concentrations 100-fold lower shows a similar effect. 65) Other evidence that PBO does not have high affinity for the esterases is that the addition of PBO to extracts containing the H. armigera esterases prior to separation by native PAGE does not affect their esterase activity.…”

Section: Heliothine Resistance To Spsmentioning

confidence: 99%

Esterase-based metabolic resistance to insecticides in heliothine and spodopteran pests

Farnsworth

Teese

Yuan

et al. 2010

Journal of Pesticide Science

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IntroductionCarboxylesterases have been implicated in metabolic resistance to several classes of insecticide in a wide variety of pest insect species. [1][2][3] The classes most commonly involved are synthetic pyrethroids (SPs) and organophosphates (OPs), and carbamates (CBs) are also involved in several cases. There are also occasional reports of carboxylesterase phenotypes associated with resistance to benzoylureas, the oxadiazine indoxacarb, the diacylhydrazine tebufenozide, the organochlorine endosulfan and the proteinaceous Cry1Ac toxin of Bacillus thuringiensis that is expressed in transgenic crops. Figure 1 gives structures for some key compounds among these various classes of insecticide.It might be expected that carboxylesterases would be involved in SP resistance because the great majority of SPs are carboxylesters. However, no precise molecular mechanisms for esterase-based SP resistance have yet been elucidated in any insect. OPs are generally phosphotriesters rather than carboxylesters but nevertheless they bind many carboxylesterases with high affinity and this provides a basis for two forms of resistance, both now elucidated at a molecular level in several species. One form involves substantial overexpression of the esterase, allowing for effective sequestration of the insecticide. The other involves specific mutations in the active site of the enzyme which convert it to an OP hydrolase (the so-called "mutant ali-esterase" mechanism). CBs are carbamic acid (NH 2 COOH) derivatives, in which a functional group has been added as an ester; these esters are chemically similar to carboxylesters. Carbamates bind carboxylesterases with quite high affinity, but as yet the molecular mechanisms by which a (mutant) carboxylesterase confers CB resistance have not been elucidated in any species. Of the other insecticide classes occasionally linked to esterase-based resistance only indoxacarb has ester bonds (two Elevated esterase activities and increased band intensities of multiple esterase isozymes after electrophoresis are commonly associated with resistance to organophosphate, pyrethroid and carbamate insecticides in various heliothine and spodopteran pests. One possible explanation for this involves a 'master regulator' mutation in a more general chemical stress response. An association between elevated esterase activities and isozyme intensities has also been reported for resistance to the Cry1Ac toxin of Helicoverpa armigera. The basis for this is unclear albeit some involvement of esterases could be mediated by the toxin's affinity for N-acetyl galactosamine glycans on certain gut-expressed esterases in this species.

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Temporal synergism by microencapsulation of piperonyl butoxide and α‐cypermethrin overcomes insecticide resistance in crop pests

Cited by 29 publications

References 21 publications

Susceptibility of four field populations of the diamondback moth Plutella xylostella L. (Lepidoptera: Yponomeutidae) to six insecticides in the Sydney region, New South Wales, Australia

Susceptibility of four field populations of the diamondback moth Plutella xylostella L. (Lepidoptera: Yponomeutidae) to six insecticides in the Sydney region, New South Wales, Australia

Efficacy of PermaNet® 3.0 and PermaNet® 2.0 nets against laboratory-reared and wild Anopheles gambiae sensu lato populations in northern Tanzania

Esterase-based metabolic resistance to insecticides in heliothine and spodopteran pests

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