Temporal leakage of Cu,Zn superoxide dismutase and loss of two low-molecular-weight forms of glutathione peroxidase-1 from buffalo (Bubalus bubalis) sperm after freezing and thawing

Kar, Senjuti; Divyashree, Bannur C.; Roy, Sudhir C.

doi:10.1016/j.theriogenology.2014.10.014

Cited by 14 publications

(8 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activity of GPx in fresh seminal plasma was higher than reported by Waheed, Gouda, and Khalifa (), which may be due to different assay method, different units of expression and bull variation. The activity of GPx was reduced in frozen‐thaw seminal plasma which was in agreement with Kar, Bannur, Divyashree, and Sudhir () who reported reduced activity of GPx in frozen spermatozoa. The higher production of ROS may be responsible for inhibition of SOD and GPx activity during freeze‐thaw procedure, which in turn may be responsible for reduced levels of these antioxidants in frozen‐thaw seminal plasma.…”

Section: Discussionsupporting

confidence: 90%

Study on correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation

et al. 2018

View full text Add to dashboard Cite

This investigation was carried out to study the correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation. Semen samples were evaluated for sperm parameters (mass motility [MM], concentration [CON], progressive motility [PM], viability [VIB], acrosomal integrity [AI] and hypo-osmotic swelling [HOS] response), antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx] and total antioxidant capacity [TAC]) and oxidants (Lipid peroxidation [LPO] and reactive oxygen species [ROS]) at fresh, pre-freeze and post-thaw stages. Sperm parameters (PM, VIB, AI and HOS response) and antioxidants (SOD, CAT and TAC) were significantly (p < .05) reduced at fresh stage, and oxidants (LPO and ROS) were significantly (p < .05) increased at pre-freeze and post-thaw stages. At fresh stage, MM was negatively correlated with LPO (p < .05), and CON was positively correlated with SOD, TAC and CAT, negatively correlated with LPO and CAT was positively (p < .01) correlated with VIB and HOS response. At pre-freeze stage, CAT was positively correlated with PM and AI (p < .05), and AI was negatively (p < .05) correlated with ROS. At post-thaw stage, CAT was positively correlated with PM, VIB, HOS response and AI,, and LPO was negatively correlated with HOS, AI and VIB. The study of correlations of these parameters at different preservation stages with bull fertility may play an important role in developing models for predicting future fertility of bulls in the absence of conception rate data.

show abstract

Section: Discussionsupporting

confidence: 90%

Study on correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Superoxide dismutase (SOD1) is an antioxidant that seeks to protect the cell from lipid peroxidation caused by free radicals [reviewed in (Kopeika et al ., )]. Similar to earlier studies performed in human, buffalo, and chicken, we found that SOD1 levels were lower following cryopreservation (Alvarez & Storey, ; Lasso et al ., ; Cheng et al ., ; Kar et al ., ). These findings were different from what was reported in pigs and fish (Sea Bream; Sparusaurata ) where SOD1 levels were increased following cryopreservation (Chen et al ., ; Zilli et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Identification of protein changes in human spermatozoa throughout the cryopreservation process

et al. 2016

View full text Add to dashboard Cite

SUMMARYCryoinjury is a consequence of cryopreservation and may have a negative impact on sperm quality regarding motility, morphology, and viability. This study was designed to identify potential proteomic changes in human sperm cells throughout the cryopreservation process. Comparisons made within this study included the detection of the sperm proteomic changes induced by incubation of the sperm cells with a protein-free cryoprotectant (with and without CryoSperm), and the proteomic changes induced by freezing, thawing, and subsequent after-thawing incubation at two different temperatures (0°C vs. 23°C). Tandem Mass Tag (TMT) peptide labeling coupled with LC-MS/MS was used for protein quantification. LC-MS/MS resulted in the identification of 769 quantifiable proteins. The abundance of 105 proteins was altered upon CryoSperm incubation. Freezing and thawing also induced substantial protein changes. However, fewer changes were observed when semen was thawed and then maintained after-thawing at approximately 0°C than when it was maintained after-thawing at 23°C, with 60 and 99 differential proteins detected, respectively, as compared to unfrozen semen incubated in CryoSperm. Collectively, these differences indicate that substantial changes occur in the sperm proteome at every stage of the cryopreservation process which may ultimately impair the sperm fertilizing capability. This is the first study to compare protein levels in fresh and cryopreserved semen using the TMT technology coupled to LC-MS/MS.

show abstract

“…Sperm cryopreservation is an indispensable tool to conserve the elite germplasm to improve farm animal species (Kar et al, 2015). Sperm recovery from the caudal epididymis can be advantageous after the unexpected death of endangered animals or animals with genetic merit.…”

Section: Introductionmentioning

confidence: 99%

The effect of quercetin on fertility of frozen-thawed ram epididymal spermatozoa

2017

View full text Add to dashboard Cite

The aim of the present study was to evaluate the effects of quercetin as an antioxidant supplement on frozen-thawed ram epididymal sperm quality. Quercetin is a type of flavonoid antioxidant that is found in plants, with the ability to scavenge free radicals. Twenty testicles from mature rams were collected from a nearby slaughterhouse immediately after slaughter. Epididymal spermatozoa were recovered from the caudal of epididymides by injecting Bracket and Oliphant's (BO) medium retrogradely through the ductus deferens and extended with a tris egg-yolk-based extender and supplemented with 0, 5, 10, 15, 20, and 50 µg/mL quercetin. Following equilibration, the straws were frozen, and then plunged into liquid nitrogen. After thawing, optimized concentrations of quercetin were defined based on their viabilities and used to assess fertilization and developmental potential. The results showed that the viability of frozen-thawed spermatozoa significantly increased by using 5 and 10 µg/mL quercetin in the freezing extender. However, total and progressive motility of frozen-thawed spermatozoa were not affected by 5 and 10 µg/mL quercetin in comparison with control (0 µg/mL). The mean number of zygote, morula, and blastocyst stage embryos increased significantly by using 5 and 10 µg/mL quercetin compared with other frozen-thawed treatments(P <0.05). However, the blastocyst rate of fresh sperm was significantly higher (P <0.05). In conclusion, to improve the quality of frozen-thawed ram epididymal spermatozoa, 5 and 10 µg/mL quercetin appears to be an attractive option. Further studies are suggested to understand the synergistic effect of quercetin with other antioxidants to improve the ram freezing-thawing process.

show abstract

Temporal leakage of Cu,Zn superoxide dismutase and loss of two low-molecular-weight forms of glutathione peroxidase-1 from buffalo (Bubalus bubalis) sperm after freezing and thawing

Cited by 14 publications

References 41 publications

Study on correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation

Study on correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation

Identification of protein changes in human spermatozoa throughout the cryopreservation process

The effect of quercetin on fertility of frozen-thawed ram epididymal spermatozoa

Contact Info

Product

Resources

About