Study on correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation

Abstract

This investigation was carried out to study the correlation of sperm quality parameters with antioxidant and oxidant status of buffalo bull semen during various stages of cryopreservation. Semen samples were evaluated for sperm parameters (mass motility [MM], concentration [CON], progressive motility [PM], viability [VIB], acrosomal integrity [AI] and hypo-osmotic swelling [HOS] response), antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione peroxidase [GPx] and total antioxidant capacity [TAC]) and oxidants (Lipid peroxidation [LPO] and reactive oxygen species [ROS]) at fresh, pre-freeze and post-thaw stages. Sperm parameters (PM, VIB, AI and HOS response) and antioxidants (SOD, CAT and TAC) were significantly (p < .05) reduced at fresh stage, and oxidants (LPO and ROS) were significantly (p < .05) increased at pre-freeze and post-thaw stages. At fresh stage, MM was negatively correlated with LPO (p < .05), and CON was positively correlated with SOD, TAC and CAT, negatively correlated with LPO and CAT was positively (p < .01) correlated with VIB and HOS response. At pre-freeze stage, CAT was positively correlated with PM and AI (p < .05), and AI was negatively (p < .05) correlated with ROS. At post-thaw stage, CAT was positively correlated with PM, VIB, HOS response and AI,, and LPO was negatively correlated with HOS, AI and VIB. The study of correlations of these parameters at different preservation stages with bull fertility may play an important role in developing models for predicting future fertility of bulls in the absence of conception rate data.

Effect of various levels of dissolved oxygen on reactive oxygen species and cryocapacitation‐like changes in bull sperm

“…This might be due to more dependence of sperm on glycolytic pathway than oxidative phosphorylation as former is less efficient than latter and there might be early exhaustion of energy sources leading to early apoptosis and death of sperm and the dead sperm further produce more ROS due to increased expression of aromatic L‐amino acid oxidase (Shannon, & Curson, ). The increase in ROS levels in frozen sperm in our study was in agreement with the reports of Chatdarong, Chaivechakarn, Thuwanut, and Ponglowhapan () in dog, Lone et al () in buffalo, but was in contrast to the reports of Kadirvel, Kumar, Ghosh, and Perumal () who revealed reduced ROS concentration in frozen‐thawed buffalo sperm than that in fresh sperm. The reasons might be different assay method used for ROS, species and breed variation.…”

“…Due to reduced seminal antioxidant levels and inefficiency of sperm to synthesize necessary constituents of the membrane, they become more sensitive to the damages of free radicals (Jones, & Mann, ). Besides this reduction in seminal antioxidant profile during cryopreservation further lowers the natural antioxidant capacity in semen (Lone et al, ). Capacitation‐like changes in sperm are induced due to the loss of membrane cholesterol during cryopreservation (Neild et al, ).…”

“…The extended semen was packaged in 0.25‐ml French mini straws (Bal Krishan Plasikrafts, India) and subjected to a combined cooling with equilibration period of 4 hr at 4°C. After equilibration, semen was frozen with a Biological Cell Freezer (IMV Technologies, L'Aigle, France) as described elsewhere (Lone et al, ). Semen was evaluated at prefreeze and post‐thaw stage for various parameters, viz.…”

“…Oxidative stress due to higher generation of reactive oxygen species (ROS) during freezing and thawing procedures is one of the most important factors responsible for impaired quality of frozen‐thawed sperm (Lone et al, ). This is due to the presence of higher amounts of polyunsaturated fatty acids (PUFAs) in the sperm plasma membrane (Sarlos, Molnar, Kokai, Gabor, & Ratky, ), and the binding of PUFAs to oxygen leads to the production of higher quantities of ROS (Gadea, Gumbao, Gomez‐Gimenez, & Gardon, ).…”

Chlorella vulgaris ameliorates testicular toxicity induced by deltamethrin in male rats via modulating oxidative stress

“…This improvement did not associate with a significant change in serum testosterone, therefore it may be concluded that testosterone is not involved in the mechanism by which CV has improved the epididymal sperm picture. Positive correlation between the oxidant/antioxidant capacity of buffalo bull ejaculate and the sperm cell concentration and motility that has been reported, supported this hypothesis (Lone et al, 2018). This indicates that higher sperm number/epididymal tail in CV-treated rats might have been occurred through increasing the number of sperm cells/mg of testicular tissue.…”