Temporal Dynamics of High-Density Lipoprotein Proteome in Diet-Controlled Subjects with Type 2 Diabetes

Kheniser, Karim; Ösme, Abdullah; Kim, Chun Ki; Ilchenko, Serguei; Kasumov, Takhar; Kashyap, Sangeeta R.

doi:10.3390/biom10040520

Cited by 13 publications

(9 citation statements)

References 66 publications

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“…Our proteomic analysis shows that A1AT is enriched in the smallest HDL fraction F6 (Fig. 6 k).VDBP is a carrier protein for Vitamin D metabolites and has been found associated with HDL fractions in some studies 41 . Most bound vitamin D metabolites are carried by VDBP and a small fraction are carried by albumin 42 .…”

Section: Discussionmentioning

confidence: 65%

Isolation of HDL by sequential flotation ultracentrifugation followed by size exclusion chromatography reveals size-based enrichment of HDL-associated proteins

Zheng

Agus

Hong

et al. 2021

Sci Rep

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High-density lipoprotein (HDL) particles have multiple beneficial and cardioprotective roles, yet our understanding of their full structural and functional repertoire is limited due to challenges in separating HDL particles from contaminating plasma proteins and other lipid-carrying particles that overlap HDL in size and/or density. Here we describe a method for isolating HDL particles using a combination of sequential flotation density ultracentrifugation and fast protein liquid chromatography with a size exclusion column. Purity was visualized by polyacrylamide gel electrophoresis and verified by proteomics, while size and structural integrity were confirmed by transmission electron microscopy. This HDL isolation method can be used to isolate a high yield of purified HDL from a low starting plasma volume for functional analyses. This method also enables investigators to select their specific HDL fraction of interest: from the least inclusive but highest purity HDL fraction eluting in the middle of the HDL peak, to pooling all of the fractions to capture the breadth of HDL particles in the original plasma sample. We show that certain proteins such as lecithin cholesterol acyltransferase (LCAT), phospholipid transfer protein (PLTP), and clusterin (CLUS) are enriched in large HDL particles whereas proteins such as alpha-2HS-glycoprotein (A2HSG), alpha-1 antitrypsin (A1AT), and vitamin D binding protein (VDBP) are enriched or found exclusively in small HDL particles.

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Section: Discussionmentioning

confidence: 65%

Isolation of HDL by sequential flotation ultracentrifugation followed by size exclusion chromatography reveals size-based enrichment of HDL-associated proteins

Zheng

Agus

Hong

et al. 2021

Sci Rep

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show abstract

“…Specifically, in the presence of type 2 diabetes, low levels of ApoA-1 and ApoE as well as reduced catabolism of apolipoprotein B (ApoB) and overexpression of ApoC-3 are observed [49]. Enrichment of HDL particles with the oxidative moieties myeloperoxidase and serum amyloid A (SAA) is also observed under diabetic conditions [50]. Lipid composition also seems to be affected by the presence of type 2 diabetes.…”

Section: Impact Of Type 2 Diabetes On Hdlmentioning

confidence: 99%

HDL and type 2 diabetes: the chicken or the egg?

et al. 2021

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HDL is a complex macromolecular cluster of various components, such as apolipoproteins, enzymes and lipids. Quality evidence from clinical and epidemiological studies led to the principle that HDL-cholesterol (HDL-C) levels are inversely correlated with the risk of CHD. Nevertheless, the failure of many cholesteryl ester transfer protein inhibitors to protect against CVD casts doubts on this principle and highlights the fact that HDL functionality, as dictated by its proteome and lipidome, also plays an important role in protecting against metabolic disorders. Recent data indicate that HDL-C levels and HDL particle functionality are correlated with the pathogenesis and prognosis of type 2 diabetes mellitus, a major risk factor for CVD. Hyperglycaemia leads to reduced HDL-C levels and deteriorated HDL functionality, via various alterations in HDL particles' proteome and lipidome. In turn, reduced HDL-C levels and impaired HDL functionality impact the performance of key organs related to glucose homeostasis, such as pancreas and skeletal muscles. Interestingly, different structural alterations in HDL correlate with distinct metabolic abnormalities, as indicated by recent data evaluating the role of apolipoprotein A1 and lecithin-cholesterol acyltransferase deficiency in glucose homeostasis. While it is becoming evident that not all HDL disturbances are causatively associated with the development and progression of type 2 diabetes, a bidirectional correlation between these two conditions exists, leading to a perpetual self-feeding cycle.

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“…Diabetes is, perhaps, the major controllable risk factor for CVD. In particular, related to the HDL fraction, ApoA-I, ApoA-II, ApoA-IV, ApoE, ApoJ, as well as PON1, transthyretin, complement C3, and vitamin D-binding protein have shown changes in patients with type 2 diabetes (T2D) [76,77]. In contrast, individuals with type 1 diabetes (T1D) had proteomic alterations of their HDL particles.…”

Section: Clinical Significance Of Lipoproteomic Analysesmentioning

confidence: 99%

Lipoproteomics: Methodologies and Analysis of Lipoprotein-Associated Proteins along with the Drug Intervention

Torres‐Romero¹,

Lara-Riegos²,

Parra³

et al. 2021

Drug Design - Novel Advances in the Omics Field and Applications

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Lipoproteins are specialized particles involved in the transport and distribution of hydrophobic lipids, as cholesterol and triglycerides, throughout the body. The lipoproteins exhibit a basic spherical shape as complexes of lipids and proteins, and these latter are known as apolipoproteins. Initially, the proteins associated with lipoproteins were recognized as integral or peripheral proteins that only maintain the dynamics and metabolism of lipoproteins. However, there exist many studies on different lipoproteins evidencing that the quantity and type of apolipoproteins and lipoprotein-associated proteins are diverse and could be associated with different lipoprotein function outcomes. Here, we summarized recent processes in the determination of apolipoproteins and lipoprotein-associated proteins profiles through a proteomic approach, analyzing the major methods available and are used to achieve this. We also discuss the relevance of these lipoproteomic analyses on the human disease outcomes.

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Temporal Dynamics of High-Density Lipoprotein Proteome in Diet-Controlled Subjects with Type 2 Diabetes

Cited by 13 publications

References 66 publications

Isolation of HDL by sequential flotation ultracentrifugation followed by size exclusion chromatography reveals size-based enrichment of HDL-associated proteins

Isolation of HDL by sequential flotation ultracentrifugation followed by size exclusion chromatography reveals size-based enrichment of HDL-associated proteins

HDL and type 2 diabetes: the chicken or the egg?

Lipoproteomics: Methodologies and Analysis of Lipoprotein-Associated Proteins along with the Drug Intervention

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