Temporal changes in microbial communities attached to forages with different lignocellulosic compositions in cattle rumen

Abstract

The attachment of rumen microbes to feed particles is critical to feed fermentation, degradation and digestion. However, the extent to which the physicochemical properties of feeds influence the colonization by rumen microbes is still unclear. We hypothesized that rumen microbial communities may have differential preferences for attachments to feeds with varying lignocellulose properties. To this end, the structure and composition of microbial communities attached to six common forages with different lignocellulosic compositions were analyzed following in situ rumen incubation in male Taleshi cattle. The results showed that differences in lignocellulosic compositions significantly affected the inter-sample diversity of forage-attached microbial communities in the first 24 h of rumen incubation, during which the highest dry matter degradation was achieved. However, extension of the incubation to 96 h resulted in the development of more uniform microbial communities across the forages. Fibrobacteres were significantly overrepresented in the bacterial communities attached to the forages with the highest neutral detergent fiber contents. Ruminococcus tended to attach to the forages with low acid detergent lignin contents. The extent of dry matter fermentation was significantly correlated with the populations of Fibrobacteraceae, unclassified Bacteroidales, Ruminococcaceae and Spirochaetacea. Our findings suggested that lignocellulosic compositions, and more specifically the cellulose components, significantly affected the microbial attachment to and thus the final digestion of the forages.

Rumen Microbiota of Tibetan Sheep (Ovis aries) Adaptation to Extremely Cold Season on the Qinghai-Tibetan Plateau

“…In this study, Bacteroidetes and Firmicutes were the most predominant bacterial phyla in the rumen of Tibetan sheep, corroborating the results of previous studies on Tibetan sheep (38,39), cattle (40), sheep (41), yaks (5,9,12), goats (42), and pikas (43), and indicating that these bacteria play an important role in the ecology and function of the mammalian gastrointestinal tract. Kim et al (44) conducted a meta-analysis of all selected 16S rRNA sequences stored in the NCBI database to summarize the distribution of rumen bacteria in major domestic animals and found that the proportions of Bacteroidetes and Firmicutes were approximately 31% and 56%, respectively.…”

Fibrolytic rumen bacteria of camel and sheep and their applications in the bioconversion of barley straw to soluble sugars for biofuel production

“…The majority of bacterial communities were assigned to phylum Firmicutes and Bacteroidetes, which agrees with previous studies conducted on lignocellulosic forages (Table 3) [17,[57][58][59][60]. Phylum Firmicutes dominated the bacterial community attached to barley straw (Table 3), which is consistent with Du et al (2019) [16] and explains that this phylum is the main contributor in lignocellulose degradation.…”

Evaluation of the Digestibility of Various Types of Swamp Grass in South Sumatra on Pampangan Local Buffalo using the in Sacco Method

“…Thus, it causes a high total digestibility in the treatment, where it can occur because the cellulose fraction is the digestible fraction in the rumen. Gharechahi et al (2020) reported that the composition of lignocellulose, or more specifically the cellulose component, can significantly affect the availability of microbes, resulting in changes in the digestibility value of the forage. Furthermore, according to the results of a research report regarding rumen fluid testing of corn straw, it shows that changes in the composition of fiber fractions in the form of hemicellulose and cellulose in the pretreatment resulted in significant changes to the quality of the resulting fermentation (Li et al, 2017).…”