Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in <i>Sambucus nigra</i><sup>*</sup>

Coupe, Simon A.; Taylor, Jane; Roberts, Jeremy A.

doi:10.1046/j.1365-3040.1997.d01-54.x

Cited by 32 publications

(11 citation statements)

References 58 publications

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“…In A. thaliana NPR1 proteins regulate the expression of PR (PATHOGENESIS-RELATED) genes (Maier et al 2011). Moreover, ethylene-induced AZ activation in Sumbucus nigra leaflet strongly increased the mRNA level of PR genes (Coupe et al 1997). Thus, it cannot be completely excluded that changes in LlBOP expression induced by exogenous phytohormones could be, in a certain degree, connected with a stress, which is one of the factors stimulating conversion leading to organ abortion.…”

Section: Resultsmentioning

confidence: 99%

Profiling the BLADE-ON-PETIOLE gene expression in the abscission zone of generative organs in Lupinus luteus

et al. 2015

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The great agronomic potential of Lupinus luteus, species widely cultivated in many European countries as well as Australia, is strongly affected by premature and excessive generative organ abscission, mainly flowers. The unwanted process takes place in a specialized group of cells, called abscission zone (AZ). During their development they become competent to respond to external and internal factors, including phytohormones. Recently it was shown that the formation of AZ cells in Arabidopsis thaliana is coordinated by transcription factors, BLADE-ON-PETIOLE (BOPs). There is no such data, excluding tobacco plants, about BOP-dependent regulation of organ abscission in crop plants. In this work, we examined LlBOP mRNA content during generative organs AZ development and functioning. The high accumulation of LlBOP transcript was accompanied by the differentiation of morphologically distinct cells at the base of the mature flower pedicel. Moreover, enhanced LlBOP expression was observed in the active AZ, and was regulated by factors, which can strongly affect generative organ abscission. All these data indicate that LlBOP is involved in the abscission zone formation and functioning in L. luteus.

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Section: Resultsmentioning

confidence: 99%

Profiling the BLADE-ON-PETIOLE gene expression in the abscission zone of generative organs in Lupinus luteus

et al. 2015

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“…The differential screen identified a number of genes that were preferentially expressed in the Cnr mutant and similar to genes expressed in Sambucus nigra leaflets during ethylene-promoted abscission (Coupe et al, 1995(Coupe et al, , 1997. Twenty Sambucus abscission-related clones were isolated from a differential screen, and sequencing revealed that the majority encoded PR proteins, including a PR-1 protein, class I chitinase, proteinase inhibitors, and a metallothionein-like protein, all of which were matched by clones from the Cnr library.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Colorless non-ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening

et al. 2004

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The Colorless non-ripening (Cnr) mutation in tomato (Solanum lycopersicum) results in mature fruits with colorless pericarp tissue showing an excessive loss of cell adhesion (A.J. Thompson, M. Tor, C.S. Barry, J. Vrebalov, C. Orfila, M.C. Jarvis, J.J. Giovannoni, D. Grierson, G.B. Seymour [1999] Plant Physiol 120: 383-390). This pleiotropic mutation is an important tool for investigating the biochemical and molecular basis of cell separation during ripening. This study reports on the changes in enzyme activity associated with cell wall disassembly in Cnr and the effect of the mutation on the program of ripening-related gene expression. Real-time PCR and biochemical analysis demonstrated that the expression and activity of a range of cell wall-degrading enzymes was altered in Cnr during both development and ripening. These enzymes included polygalacturonase, pectinesterase (PE), galactanase, and xyloglucan endotransglycosylase. In the case of PE, the protein product of the ripening-related isoform PE2 was not detected in the mutant. In contrast with wild type, Cnr fruits were rich in basic chitinase and peroxidase activity. A microarray and differential screen were used to profile the pattern of gene expression in wild-type and Cnr fruits. They revealed a picture of the gene expression in the mutant that was largely consistent with the real-time PCR and biochemical experiments. Additionally, these experiments demonstrated that the Cnr mutation had a profound effect on many aspects of ripening-related gene expression. This included a severe reduction in the expression of ripening-related genes in mature fruits and indications of premature expression of some of these genes in immature fruits. The program of gene expression in Cnr resembles to some degree that found in dehiscence or abscission zones. We speculate that there is a link between events controlling cell separation in tomato, a fleshy fruit, and those involved in the formation of dehiscence zones in dry fruits.Colorless non-ripening (Cnr) is a pleiotropic dominant mutation of tomato (Solanum lycopersicum) that results in fruits with a white pericarp displaying much reduced cell-to-cell adhesion (Thompson et al., 1999;Fraser et al., 2001). The Cnr locus has been mapped to the middle of the long arm of chromosome 2 and is currently the subject of a map-based cloning exercise (Tor et al., 2002). We have recently isolated and sequenced a region of tomato chromosome 2 that cosegregates with the Cnr locus and are testing a candidate gene at this locus (K. Manning, J.J. Giovannoni, and G.B. Seymour, unpublished data).The loss of cell adhesion in Cnr appears to be due principally to modifications in cell wall structure. Sections of Cnr pericarp tissue show obvious changes in comparison with wild-type fruits, including larger intercellular spaces and thinner cell walls in ripe fruits (Orfila et al., 2001). Mechanical tests on pericarp tissue have revealed that the force required for cell wall failure is greater in Cnr, while tests on cell wall preparations showed ...

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“…Based on expression patterns and modulation in transgenic plants and mutants, genes encoding polygalacturonases (PGs) and b-1,4-glucanases (cellulases) have been suggested to play a central role in the execution of cell separation (Greenberg et al, 1975;Lashbrook et al, 1998;Brummell et al, 1999;Hong et al, 2000;Gonzá lez-Carranza et al, 2007;Jiang et al, 2008;Ogawa et al, 2009). Other proteins associated with the AZ are expansin (Cho and Cosgrove, 2000;Belfield et al, 2005), pathogenesis-related proteins (Eyal et al, 1993;Coupe et al, 1997), and metallothioneins (Coupe et al, 1995), but their role in the process is not yet clear.…”

Section: Introductionmentioning

confidence: 99%

Programmed Cell Death Occurs Asymmetrically during Abscission in Tomato

et al. 2011

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Abscission occurs specifically in the abscission zone (AZ) tissue as a natural stage of plant development. Previously, we observed delay of tomato (Solanum lycopersicum) leaf abscission when the LX ribonuclease (LX) was inhibited. The known association between LX expression and programmed cell death (PCD) suggested involvement of PCD in abscission. In this study, hallmarks of PCD were identified in the tomato leaf and flower AZs during the late stage of abscission. These included loss of cell viability, altered nuclear morphology, DNA fragmentation, elevated levels of reactive oxygen species and enzymatic activities, and expression of PCD-associated genes. Overexpression of antiapoptotic proteins resulted in retarded abscission, indicating PCD requirement. PCD, LX, and nuclease gene expression were visualized primarily in the AZ distal tissue, demonstrating an asymmetry between the two AZ sides. Asymmetric expression was observed for genes associated with cell wall hydrolysis, leading to AZ, or associated with ethylene biosynthesis, which induces abscission. These results suggest that different abscission-related processes occur asymmetrically between the AZ proximal and distal sides. Taken together, our findings identify PCD as a key mechanism that occurs asymmetrically during normal progression of abscission and suggest an important role for LX in this PCD process.

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Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra^*

Cited by 32 publications

References 58 publications

Profiling the BLADE-ON-PETIOLE gene expression in the abscission zone of generative organs in Lupinus luteus

Profiling the BLADE-ON-PETIOLE gene expression in the abscission zone of generative organs in Lupinus luteus

Effect of the Colorless non-ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening

Programmed Cell Death Occurs Asymmetrically during Abscission in Tomato

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Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra*

Cited by 32 publications

References 58 publications

Profiling the BLADE-ON-PETIOLE gene expression in the abscission zone of generative organs in Lupinus luteus

Profiling the BLADE-ON-PETIOLE gene expression in the abscission zone of generative organs in Lupinus luteus

Effect of the Colorless non-ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening

Programmed Cell Death Occurs Asymmetrically during Abscission in Tomato

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Temporal and spatial expression of mRNAs encoding pathogenesis‐related proteins during ethylene‐promoted leaflet abscission in Sambucus nigra^*