Template assisted synthesis of porous nanofibrous cellulose membranes for tissue engineering

Rambo, Carlos R.; Recouvreux, Derce de Oliveira Souza; Carminatti, Claudimir Antonio; Pitlovanciv, Ana Kelly; Antônio, Regina Vasconcellos; Porto, Luismar Marques

doi:10.1016/j.msec.2007.11.011

Cited by 127 publications

(58 citation statements)

References 32 publications

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“…In its natural state, BNC is known to be impenetrable by cells, due to the small size of the pores Klemm et al, 2005). However, this issue has been addressed by several investigators, who successfully synthesized BNC scaffolds with large pores, allowing the cells to penetrate the scaffold (Bäckdahl et al, 2008;Rambo et al, 2008). Therefore, in the scope of this work the mechanical 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 5 properties of BNC are the primary focus, including investigation of the tunability of the natural material.…”

Section: Introductionmentioning

confidence: 99%

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Nimeskern

Ávila

Sundberg

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

197

109

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3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Secondly, the capacity of BNC to match these requirements is assessed. Finally, a biofabrication process to produce patient-specific BNC auricular implants is demonstrated.BNC samples (n = 78) with varying cellulose content (2.5 -15%) were compared using stressrelaxation indentation with human ear cartilage (n = 17, from 4 males, aged 49 -93 years old).Additionally, an auricle from a volunteer was scanned using a 3T MRI with a spoiled gradientecho sequence. A negative ear mold was produced from the MRI data in order to investigate if an ear-shaped BNC prototype could be produced from this mold.The results show that the instantaneous modulus E in , equilibrium modulus E eq , and maximum stress σ max of the BNC samples are correlated to effective cellulose content. Despite significantly different relaxation kinetics, the E in , E eq and σ max of BNC at 14% effective cellulose content reached values equivalent to ear cartilage (for E eq , BNC: 2.4 ± 0.4 MPa and ear cartilage: 3.3 ± 1.3 MPa). Additionally, this work shows that BNC can be fabricated into patient-specific auricular shapes. In conclusion, BNC has the capability to reach mechanical properties of relevance for ear cartilage replacement, and can be produced in patient-specific ear shapes.

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Section: Introductionmentioning

confidence: 99%

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Nimeskern

Ávila

Sundberg

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

197

109

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“…It is biocompatible [11] and has good mechanical properties despite its high water content. Bacterial cellulose has been used as scaffolds for tissue engineered meniscus and blood vessels [11][12][13][14]. Studies with endothelial cells, smooth muscle cells and chondrocytes have shown that these cells adhere to the bacterial cellulose.…”

Section: Introductionmentioning

confidence: 99%

Microporous bacterial cellulose as a potential scaffold for bone regeneration

et al. 2010

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a b s t r a c tNanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300-500 lm paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young's modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications.

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“…Para a biossíntese de celulose bacteriana foram seguidos os métodos já desenvolvidos por Rambo et al e Recouvreux et al [20,21] , que consistem basicamente da inoculação de bactérias em um volume de 10% em relação ao volume do meio de cultura; crescimento estático e à temperatura ambiente (25 °C) em um período de 7 dias. Para a obtenção de tubos cilíndricos de celulose bacteriana (vasos de CB) construiu-se um aparato em silicone, como mostrado na Figura 1.…”

Section: Methodsunclassified

Produção e degradação in vitro de estruturas tubulares de celulose bacteriana

Oliveira¹,

Rambo²,

Porto³

2013

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Resumo: A necessidade de novos recursos em cardiologia tem direcionado a Engenharia de Tecidos ao desenvolvimento de vasos sanguíneos artificiais que atendam aos requisitos do organismo. Neste trabalho, estruturas tubulares de celulose bacteriana (CB) foram produzidas e sua degradação in vitro foi avaliada. Através de microscopia eletrônica de varredura constatou-se que não houve alterações significativas na microestrutura e morfologia das fibras de CB após ensaios de degradação. Os ensaios de degradação em soluções fisiológicas PBS e salina revelaram uma degradação substancialmente baixa após 20 semanas. A baixa velocidade de degradação dos vasos é de grande importância, visto que o processo para a formação de novos vasos (angiogênese) demanda tempo. Palavras-chave: Celulose bacteriana, biodegradação, vasos sanguíneos artificiais. Production and In Vitro Degradation of Bacterial Cellulose Tubular StructuresAbstract: The need of new techniques in cardiology has driven Tissue Engineering towards the development of artificial blood vessels that fulfill the requirements of the organism. In this work bacterial cellulose (BC) tubular structures were produced and their degradation in vitro was evaluated. No significant changes in microstructure and BC fiber morphology were observed by scanning electron microscopy after degradation tests. Degradation tests in physiological solutions (PBS and saline) revealed a very low degradation after 20 weeks. A low degradation rate of artificial vessels is important, since the process of new blood vessel formation (angiogenesis) demands time.

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Template assisted synthesis of porous nanofibrous cellulose membranes for tissue engineering

Cited by 127 publications

References 32 publications

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Microporous bacterial cellulose as a potential scaffold for bone regeneration

Produção e degradação in vitro de estruturas tubulares de celulose bacteriana

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