Microporous bacterial cellulose as a potential scaffold for bone regeneration

Zaborowska, Magdalena; Bodin, Aase Katarina; Bäckdahl, Henrik; Popp, Jenni R.; Goldstein, Aaron S.; Gatenholm, Paul

doi:10.1016/j.actbio.2010.01.004

Cited by 347 publications

(210 citation statements)

References 32 publications

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“…It is known that oxidation of fibrous cellulose leads to some loss of material (presumably by dissolution) and individualisation of fibrils, thus a film of relatively high DO was selected for comparison (Jin et al 2014). The Young's modulus for the unmodified cellulose films was 2 ± 0.8 MPa [comparable with previously reported value of 1.6 MPa (Zaborowska et al 2010)] and did not change significantly upon modification (Fig. 2a).…”

Section: Mechanical Propertiessupporting

confidence: 65%

“…In tissue engineering it is important that the scaffold has a similar E to the surrounding tissue so that it can cope with mechanical wear and also to guide stem cell differentiation (Engler et al 2006). The value of E & 2 MPa measured for these cationic cellulose films suggests potential for application in scaffolds for soft tissues or non-weight bearing bone (Zaborowska et al 2010). …”

Section: Discussionmentioning

confidence: 99%

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Surface modified cellulose scaffolds for tissue engineering

et al. 2016

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We report the ability of cellulose to support cells without the use of matrix ligands on the surface of the material, thus creating a two-component system for tissue engineering of cells and materials. Sheets of bacterial cellulose, grown from a culture medium containing Acetobacter organism were chemically modified with glycidyltrimethylammonium chloride or by oxidation with sodium hypochlorite in the presence of sodium bromide and 2,2,6,6-tetramethylpipiridine 1-oxyl radical to introduce a positive, or negative, charge, respectively. This modification process did not degrade the mechanical properties of the bulk material, but grafting of a positively charged moiety to the cellulose surface (cationic cellulose) increased cell attachment by 70% compared to unmodified cellulose, while negatively charged, oxidised cellulose films (anionic cellulose), showed low levels of cell attachment comparable to those seen for unmodified cellulose. Only a minimal level of cationic surface derivitisation (ca 3% degree of substitution) was required for increased cell attachment and no mediating proteins were required. Cell adhesion studies exhibited the same trends as the attachment studies, while the mean cell area and aspect ratio was highest on the cationic surfaces. Overall, we demonstrated the utility of positively charged bacterial cellulose in tissue engineering in the absence of proteins for cell attachment.

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Section: Mechanical Propertiessupporting

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Surface modified cellulose scaffolds for tissue engineering

et al. 2016

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“…16,17,22 Its unique properties have sustained the elevator pitch of several BC applications, especially in the biomedical field, where temporary skin substitutes and artificial blood vessels appear as patented products (such as Biofill and BASYC). Recent studies on the potential use of BC as a biomaterial include artificial skin 17 , vascular grafts 20,23,24 , conduits in urinary reconstruction and diversion 25 , cartilage replacement 26 , bone regeneration 27 , artificial cornea 28 , tissue engineering hydrogels 29 and scaffolds 30 . Also BC is not biodegradable in the human body, which can be beneficial since substrates developed with degradable materials and biological tissues can be difficult to handle and may induce retinal degeneration due to material degradation.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Gonçalves¹,

Padrão²,

Rodrigues

et al. 2015

Biomacromolecules

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The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 μm of average thickness) and heat-dried BC substrates were surface-modified via acetylation and polysaccharide adsorption, using chitosan and carboxymethyl cellulose. All substrates were characterized according to their surface chemistry, wettability, energy, topography, and also regarding their permeability, dimensional stability, mechanical properties, and endotoxin content. Then, their ability to promote RPE cell adhesion and proliferation in vitro was assessed. All surface-modified BC substrates presented similar permeation coefficients with solutes of up to 300 kDa. Acetylation of BC decreased it's swelling and the amount of endotoxins. Surface modification of BC greatly enhanced the adhesion and proliferation of RPE cells. All samples showed similar stress-strain behavior; BC and acetylated BC showed the highest elastic modulus, but the latter exhibited a slightly smaller tensile strength and elongation at break as compared to pristine BC. Although similar proliferation rates were observed among the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation. 2 ABSTRACT: The feasibility of bacterial cellulose (BC) as a novel substrate for retinal pigment epithelium (RPE) culture was evaluated. Thin (41.6 ± 2.2 µm of average thickness) and heatdried BC substrates were surface modified via acetylation and polysaccharide adsorption using chitosan and carboxymethyl cellulose. The BC substrates were characterized according to surface chemistry, wettability, energy, topography, permeability, dimensional stability, mechanical properties and level of endotoxins present. Then, the ability to promote RPE cell adhesion and proliferation in vitro was assessed. BC substrates were porous and permeable to solutes up to 300 kDa. The acetylation decreased substrate swelling and the amount of endotoxins present. Surface modification greatly enhanced the adhesion and proliferation of RPE cells in BC. Although similar proliferation rates were observed between the modified substrates, the acetylated ones showed higher initial cell adhesion. This difference may be mainly due to the moderately hydrophilic surface obtained after acetylation.

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“…and in-vitro tissue regeneration (e.g. cortical bone [12], muscle [13,14], peripheral nerves [15], etc.). Still, the incomplete knowledge of their mechanical characteristics, especially time-dependent behaviour, complicates understanding of their performance under various loading conditions.…”

Section: Introductionmentioning

confidence: 99%

Through-thickness stress relaxation in bacterial cellulose hydrogel

Gao

Kuśmierczyk

Shi

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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Biological hydrogels, e.g. bacterial cellulose (BC) hydrogel, attracted increasing interest in recent decades since they show a good potential for biomedical engineering as replacements of real tissues thanks mainly to their good biocompatibility and fibrous structure. To select potential candidates for such applications, a comprehensive understanding of their performance under application-relevant conditions is needed. Most hydrogels demonstrate time-dependent behaviour due to the contribution of their liquid phase and reorientation of fibres in a process of their deformation. To quantify such time-dependent behaviour is crucial due to their exposure to complicated loading conditions in body environment. Some hydrogel-based biomaterials with a multi-layered fibrous structure demonstrate a promise as artificial skin and blood vessels. The results show a good potential to use a fraction-exponential model to describe such behaviour of multi-layered hydrogels, especially at stages of stress decay at low forces and of stress equilibrium at high forces.

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Microporous bacterial cellulose as a potential scaffold for bone regeneration

Cited by 347 publications

References 32 publications

Surface modified cellulose scaffolds for tissue engineering

Surface modified cellulose scaffolds for tissue engineering

Bacterial Cellulose As a Support for the Growth of Retinal Pigment Epithelium

Through-thickness stress relaxation in bacterial cellulose hydrogel

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