Temperature-stress Pretreatment in Barley Anther Culture

Huang, Bin; Sunderland, N.

doi:10.1093/oxfordjournals.aob.a086232

Cited by 131 publications

(44 citation statements)

References 4 publications

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“…Methods of spike collection, pre-treatment and culture were as described by Huang and Sunderland (1982). Spontaneously doubled baploids were produced from the parental material and self pollinated to produce a total of 74 distinct microspore-derived (M) lines.…”

Section: Methodsmentioning

confidence: 99%

Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare CV. Sabarlis

et al. 1984

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SUMMARYSeventy-four spontaneously doubled microspore-derived (M) lines of Hordeum vulgare cv. Sabarlis were grown in the field and their agronomic performance compared with that of 16 selfed seed-derived (S) lines. It was found that two of the characters scored, namely mildew susceptibility, and growth habit did not show any differences between S and M lines. In contrast, the other seven characters, days to awn emergence, plant height, neck length, number of fertile tillers, number of grains on the main tiller, grain yield on the main tiller, and thousand grain weight, all showed significant differences between the M lines and not between the S lines. Overall, the M populations tended to be later to awn emergence, and to be taller with longer necks and fewer tillers than the S population.Possible explanations for this variation are discussed in relation to other examples of "somaclonal variation" described amongst plants regenerated from cultured cells.

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Section: Methodsmentioning

confidence: 99%

Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare CV. Sabarlis

et al. 1984

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“…Spikes containing anthers in the mid uninucleate stage were cold pretreated at 4 ~ for 28 days in two-compartment Petri dishes (Sterilin 501) as described by HUANG and SUNDERLAND (11).…”

Section: Tissue Culturementioning

confidence: 99%

Induction of microspore embryogenesis in cultured anthers of hordeum vulgare. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources

Olsen

1987

Carlsberg Res. Commun.

118

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Improvement of the yield of green plants was obtained by decreasing the concentration of ammonium nitrate in the culture medium and using glutamine as a nontoxic nitrogen source. Embryoids developed directly from the microspores on such media, and frequently green plants were obtained by germination of the embryoids on the induction medium, whereas formation of calli was greatly reduced.Ficoll was tested as a carrier agent in liquid barley anther culture. It gave a higher total yield ofembryoids resulting from an increased number of responding anthers and an increased number of developed structures. When anthers were floated on a Ficoll medium surface the microspores developed inside the anther. After several mitotic divisions the anther wall burst and the proembryoids were expelled onto the surface of the medium. Here their development continued by germination. The substitution of ammonium nitrate by glutamine or asparagine in the liquid medium containing Ficoll was found to be beneficial for culture development.This report is based on the regeneration of 6,827 green plants, with an optimal average ratio of green to albino plants of 1.8. A frequency of 4.6 green plants per plated anther was obtained.

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“…Immediately after harvesting, tillers were sterilized in the laminar flow with ethanol 96 o . Spikes were removed and stored at 4 o C, in the dark, as described by Huang & Sunderland (1982).…”

Section: Methodsmentioning

confidence: 99%

Ontogenetic events in androgenesis of Brazilian barley genotypes

2000

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This paper describes a simple procedure for obtention of barley (Hordeum vulgare L.) doubled haploid plants from Brazilian hybrid genotypes. Anatomical and histological examinations showed the reversion of barley pollen to an sporophytic mode of development. A sequence of mitosis led to the formation of multicellular pollen grains. Regeneration of plants occurred either by direct embryogenesis or callus formation followed by differentiation through direct embryogenesis or organogenesis. Plants were formed in the same medium used for induction dispensing an additional regeneration step. This procedure makes doubled haploid production simpler and faster. Plantlets were transferred to another medium for rooting and after that planted in pots with vermiculite and nutrient solution.Key words: anther culture, doubled haploids. RESUMO Método para obtenção de duplo-haplóides de genótipos brasileiros de cevadaAnteras de cevada (Hordeum vulgare L.) foram cultivadas in vitro, fazendo com que houvesse uma reversão no desenvolvimento dos micrósporos para um modo esporofítico de desenvolvimento. Uma primeira mitose simétrica, seguida de uma seqüência de mitoses, levou à formação de micrósporos multinucleados. A regeneração de plantas ocorreu tanto por embriogênese direta quanto pela formação de calos, com posterior diferenciação via embriogênese indireta ou organogênese. Os embriões e calos androgenéticos mostraram grande potencial para regeneração, e plantas verdes e albinas foram regeneradas no mesmo meio utilizado para indução, sendo a transferência para outro meio necessária somente para dar prosseguimento ao desenvolvimento e ao enraizamento das plântulas. A possibilidade de induzir pró-embrióides e regenerar plantas em um único passo, sem necessidade de transferência para um meio específico de regeneração, aumenta a rapidez e a eficiência da técnica de cultura de anteras.Palavras-chave: cultura de anteras, duplo-haplóides.

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Temperature-stress Pretreatment in Barley Anther Culture

Cited by 131 publications

References 4 publications

Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare CV. Sabarlis

Variation in the agronomic characters of microspore-derived plants of Hordeum vulgare CV. Sabarlis

Induction of microspore embryogenesis in cultured anthers of hordeum vulgare. The effects of ammonium nitrate, glutamine and asparagine as nitrogen sources

Ontogenetic events in androgenesis of Brazilian barley genotypes

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