Temperature stress and insect immunity

Wojda, Iwona

doi:10.1016/j.jtherbio.2016.12.002

Cited by 96 publications

(60 citation statements)

References 82 publications

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“…Some proteins with other putative pleiotropic functions may also play a role in termite immunity, as has been shown in other insects. These include proteases (Colbert et al ., ), protease inhibitors (Cerenius et al ., ), stress response proteins (Napping, ; de Morais Guedes et al ., ; Colinet et al ., ; Wojda, ) and oxidoreductases like glucose dehydrogenase [FAD‐quinone] in the larvae of the moth M. sexta (Cox‐Foster & Stehr, ). The top 30 proteins, as sorted by their relative abundance and categorized by function [based on exponentially modified protein abundance index (emPAI) value], are listed in Table .…”

Section: Resultsmentioning

confidence: 99%

Termite soldiers contribute to social immunity by synthesizing potent oral secretions

Johnston

Kuropka

et al. 2018

Insect Molecular Biology

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The importance of soldiers to termite society defence has long been recognized, but the contribution of soldiers to other societal functions, such as colony immunity, is less well understood. We explore this issue by examining the role of soldiers in protecting nestmates against pathogen infection. Even though they are unable to engage in grooming behaviour, we find that the presence of soldiers of the Darwin termite, Mastotermes darwiniensis, significantly improves the survival of nestmates following entomopathogenic infection. We also show that the copious exocrine oral secretions produced by Darwin termite soldiers contain a high concentration of proteins involved in digestion, chemical biosynthesis, and immunity. The oral secretions produced by soldiers are sufficient to protect nestmates against infection, and they have potent inhibitory activity against a broad spectrum of microbes. Our findings support the view that soldiers may play an important role in colony immunity, and broaden our understanding of the possible function of soldiers during the origin of soldier-first societies.

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Section: Resultsmentioning

confidence: 99%

Termite soldiers contribute to social immunity by synthesizing potent oral secretions

Johnston

Kuropka

et al. 2018

Insect Molecular Biology

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“…In the present study, only male sperm production was affected by the temperature causing no consequences for competitive mating ability. It is possible that the high temperature treatment did not affect all the physiological functions of males, such as breathing, the nervous system and general behavior (Neven, 2000;Wojda, 2016) which otherwise would have made males weaker and less likely to mate with females. The fact that measured fitness was not reduced may also result from the present experimental design, which did not test successive mating of males throughout their lives or competition in natural situations involving numerous males and females emerging simultaneously in seed stocks (Ahmed, 1996).…”

Section: Discussionmentioning

confidence: 99%

Heat shock sensitivity of adult male fertility in the parasitoid wasp Anisopteromalus calandrae (Hymenoptera, Pteromalidae)

Chevrier

Nguyen

Bressac

2019

Journal of Thermal Biology

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In insects, decreased reproduction is a sublethal consequence of high temperatures, with males being more sensitive to this in many species. In hymenoptera, arrhenotokous parthenogenesis means that female offspring are produced using sperm and are thus diploid, while males are haploid. Consequently, sperm stocks in males and females (after copulation) are a key regulator of the sex ratio. Anisopteromalus calandrae is a parasitoid wasp in which males can suffer from subfertility due to a drastic decrease in sperm count after exposure to high temperatures during a critical early pupal stage. However, in this species spermatogenesis continues during adulthood, therefore the heat sensitivity of adult males remains to be studied. Laboratory studies were conducted on virgin and previously mated young adult males under control (30°C) and heat shock (10 min at 48°C) conditions to exhaust their initial sperm stock. After heat shock, in both virgin and already mated males, the individual sperm potential was half that of controls. Both groups continuously produced sperm, but sperm stock of heat shocked males' never reached that of the controls. Heat shock reduced survival at 10 days only in previously experienced males but had no impact on the mating ability in competition for a female compared to controls. Despite a reduced sperm count, heat shocked males had fully fertile spermatozoa. Such a physiological response to heat shock in a species with continuous sperm production could be of major interest for both wild populations in a context of temperature variations and parasitoid wasps introduced for agronomical purposes.

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“…Although they are mainly located in the mitochondria of eukaryotic cells, HSP60 proteins are also found in the cytosol and endoplasm (Li et al;2017). The gene expression profile of HSP60 in insects is not as commonly studied as those of HSP70 and 90 because the expression of this protein cannot be induced by heat stress in certain species (Hai-Hong et al, 2014;Li et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The phenomenon of thermal shock was first described in the fruit fly Drosophila melanogaster by Ritossa (1962); this led to the discovery of HSPs (Tissières et al, 1974), which are common and related to immunity (Wojda, 2017). In insects, these proteins are expressed after exposure to abiotic stressors, such as heavy metals, ultraviolet radiation, chemical compounds, hypoxia, dehydration and inanition, as well as biological stressors such as parasites, pathogens and hormones.…”

Section: Introductionmentioning

confidence: 99%

Research Article Heat shock genes in the stingless bee <i>Melipona interrupta</i> (Hymenoptera, Meliponini)

Siqueira¹,

Brito²,

Carvalho‐Zilse³

2018

Genet. Mol. Res.

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Heat shock proteins (HSPs) are highly conserved molecules across all plant and animal species. In insects, HSPs are expressed in response to biotic and abiotic stressors and have a well characterized expression pattern in response to heat stress, especially heat shock genes Hsp60, 70 and 90. Temperature affects many aspects of eusocial Hymenoptera, including the stingless bees (Apidae, Meliponini). Consequently, these insects have developed thermal adaptation mechanisms, including thermoregulation. However, this ability decreases when there is deviation from the optimum temperature, compromising colony survival. The study of molecular responses to heat shock stress can be important for the preservation of these pollinizers. We identified and validated in silico the genes encoding HSP60, 70 and 90 in Melipona interrupta, one of the main stingless bees used for honey production in the Amazon region. cDNA fragments of males, workers and queen bees at the white-eyed pupal stage were amplified using degenerate primers. After sequencing, aligning and editing, the sequences were compared with public genomic databases for in silico validation. One fragment of Hsp60, three fragments of Hsp70 and two fragments of Hsp90 were obtained for M. interrupta. These fragments showed 100% ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 17 (3): gmr18062 T.C.S. Siqueira et al 2 similarity with mitochondrial sequences of HSP60 and cytosolic sequences of HSP70 and HSP90 of bees of the genera Apis and Bombus. Therefore, the fragments obtained in this study correspond to parts of HSP60 (mitochondria), HSP70 and HSP90 (cytosol) in white-eyed pupae of M. interrupta. The nucleotide sequences of these fragments did not vary between genders and castes. Our validation in silico of the genes encoding HSPs will be useful for future investigations regarding differential expression of HSPs in response to environmental factors that affect the development, maintenance and survival of stingless bee colonies in the Amazon.

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Temperature stress and insect immunity

Cited by 96 publications

References 82 publications

Termite soldiers contribute to social immunity by synthesizing potent oral secretions

Termite soldiers contribute to social immunity by synthesizing potent oral secretions

Heat shock sensitivity of adult male fertility in the parasitoid wasp Anisopteromalus calandrae (Hymenoptera, Pteromalidae)

Research Article Heat shock genes in the stingless bee <i>Melipona interrupta</i> (Hymenoptera, Meliponini)

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