Temperature sensitivity of the cdc9-1 allele of Saccharomyces cerevisiae DNA ligase is dependen on specific combinations of amino acids in the primary structure of the expressed protein

Unternährer, S.; Hinnen, Albert

doi:10.1007/bf00280014

Cited by 3 publications

(1 citation statement)

References 6 publications

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“…We studied genetic interactions of UBP10 with a conditional allele of the DNA ligase I by testing the viability of cdc9 ts and ubp10 Δ double mutant cells. In our study we used a cdc9-7 allele (from the National BioResource Project, NBRP Japan) a W303 derivative strain that we characterized and sequenced to find that it is synonymous to the cdc9-1 allele 53 . We found that depletion of Ubp10 aggravates dramatically the thermosensitivity of strains carrying this CDC9 temperature-sensitive mutant allele (Figure 3A).…”

Section: Resultsmentioning

confidence: 99%

Timely lagging strand maturation relies on Ubp10-mediated PCNA dissociation from replicating chromatin

Zamarreño,

Muñoz,

Alonso

et al. 2024

Preprint

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Synthesis and maturation of Okazaki Fragments is an incessant and highly efficient metabolic process completing the synthesis of the lagging strands at replication forks during S phase. Accurate Okazaki fragment maturation (OFM) is crucial to maintain genome integrity and, therefore, cell survival in all living organisms. In eukaryotes, OFM involves the consecutive action of DNA polymerase Pol ∂, 5’ Flap endonuclease Fen1 and DNA ligase I, and constitutes the best example of a sequential process coordinated by the sliding clamp PCNA. For OFM to occur efficiently, cooperation of these enzymes with PCNA must be highly regulated. Here, we present evidence of a role for the PCNA-deubiquitylase Ubp10 in the maturation of Okazaki fragments in the budding yeastSaccharomyces cerevisiae. We show that Ubp10 associates with lagging-strand DNA synthesis machineries on replicating chromatin to ensure timely ligation of Okazaki fragments by promoting an Elg1ATAD5-independent PCNA unloading mechanism.This document was written without the use of AI.

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Section: Resultsmentioning

confidence: 99%

Timely lagging strand maturation relies on Ubp10-mediated PCNA dissociation from replicating chromatin

Zamarreño,

Muñoz,

Alonso

et al. 2024

Preprint

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Timely lagging strand maturation relies on Ubp10 deubiquitylase-mediated PCNA dissociation from replicating chromatin

Zamarreño,

Muñoz,

Alonso-Rodríguez

et al. 2024

Nat Commun

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Synthesis and maturation of Okazaki Fragments is an incessant and highly efficient metabolic process completing the synthesis of the lagging strands at replication forks during S phase. Accurate Okazaki fragment maturation (OFM) is crucial to maintain genome integrity and, therefore, cell survival in all living organisms. In eukaryotes, OFM involves the consecutive action of DNA polymerase Pol ∂, 5’ Flap endonuclease Fen1 and DNA ligase I, and constitutes the best example of a sequential process coordinated by the sliding clamp PCNA. For OFM to occur efficiently, cooperation of these enzymes with PCNA must be highly regulated. Here, we present evidence of a role for the K164-PCNA-deubiquitylase Ubp10 in the maturation of Okazaki fragments in the budding yeast Saccharomyces cerevisiae . We show that Ubp10 associates with lagging-strand DNA synthesis machineries on replicating chromatin to ensure timely ligation of Okazaki fragments by promoting PCNA dissociation from chromatin requiring lysine 164 deubiquitylation.

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The Impact of Lagging Strand Replication Mutations on the Stability of CAG Repeat Tracts in Yeast

2000

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We have examined the stability of long tracts of CAG repeats in yeast mutants defective in enzymes suspected to be involved in lagging strand replication. Alleles of DNA ligase (cdc9-1 and cdc9-2) destabilize CAG tracts in the stable tract orientation, i.e., when CAG serves as the lagging strand template. In this orientation nearly two-thirds of the events recorded in the cdc9-1 mutant were tract expansions. While neither DNA ligase allele significantly increases the frequency of tract-length changes in the unstable orientation, the cdc9-1 mutant produced a significant number of expansions in tracts of this orientation. A mutation in primase (pri2-1) destabilizes tracts in both the stable and the unstable orientations. Mutations in a DNA helicase/deoxyribonuclease (dna2-1) or in two RNase H activities (rnh1Δ and rnh35Δ) do not have a significant effect on CAG repeat tract stability. We interpret our results in terms of the steps of replication that are likely to lead to expansion and to contraction of CAG repeat tracts.

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Temperature sensitivity of the cdc9-1 allele of Saccharomyces cerevisiae DNA ligase is dependen on specific combinations of amino acids in the primary structure of the expressed protein

Cited by 3 publications

References 6 publications

Timely lagging strand maturation relies on Ubp10-mediated PCNA dissociation from replicating chromatin

Timely lagging strand maturation relies on Ubp10-mediated PCNA dissociation from replicating chromatin

Timely lagging strand maturation relies on Ubp10 deubiquitylase-mediated PCNA dissociation from replicating chromatin

The Impact of Lagging Strand Replication Mutations on the Stability of CAG Repeat Tracts in Yeast

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