Temperature ranges over which rainbow trout fibroblasts survive and synthesize heat‐shock proteins

Mosser, Dick D.; Heikkila, John J.; Bols, Niels C.

doi:10.1002/jcp.1041280312

Cited by 70 publications

(43 citation statements)

References 38 publications

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“…But cells from poikilothermic teleosts are not able to grow at their sub-lethal temperatures (Hightower and Renfro 1988). For example, the rainbow trout, Oncorhynchus mykiss, cell line RTG-2 cannot grow at 26-28°C and cannot survive at 30°C (Mosser et al 1986). It has been also reported that goldfish, Carassius auratus, cells easily change their growth rate in association with temperature shifts (Sato et al 1990).…”

Section: Discussionmentioning

confidence: 99%

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

et al. 2014

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A new epithelial cell line, Horabagrus brachysoma fin (HBF), was established from the caudal fin tissue of yellow catfish, H. brachysoma and characterized. This HBF cell line was maintained in Leibovitz's-15 medium supplemented with 15 % fetal bovine serum (FBS) and subcultured more than 62 times over a period of 20 months. The HBF cell line consists predominantly of epithelial cells and is able to grow at temperatures between 20 and 35°C with an optimum temperature of 28°C. The growth rate of these cells increased as the proportion of FBS increased from 5 to 20 % at 28°C with optimum growth at the concentrations of 15 % FBS. Partial amplification and sequencing of fragments of two mitochondrial genes 16S rRNA and COI confirmed that HBF cell line originated from yellow catfish. The HBF cells showed strong positive reaction to the cytokeratin marker, indicating that it was epithelial in nature. HBF cell line was inoculated with tissue homogenate from juveniles of Sea bass, Lates calcarifer infected with viral nervous necrosis virus (VNNV) and found not susceptible to VNNV. The extracellular products of Vibrio cholerae MTCC 3904 were toxic to the HBF cells. These cells were confirmed for the absence of Mycoplasma sp by PCR.

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Section: Discussionmentioning

confidence: 99%

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

et al. 2014

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“…Among the environmental stressors that aquatic vertebrates have to face, temperature changes have a major role for the growth and survival of ectothermic animals living in variable thermal environments (Iwama et al 1998;Mosser et al 1986). HSPs participate in chaperone-mediated autophagy (CMA), a chaperone-dependent selection of oxidized and abnormal proteins produced under stressed conditions and targeted to lysosomes for degradation.…”

Section: Autophagy Induced By Environmental Stress On Aquatic Invertementioning

confidence: 99%

Autophagy as a defense strategy against stress: focus on Paracentrotus lividus sea urchin embryos exposed to cadmium

Chiarelli

Martino

Agnello

et al. 2016

Cell Stress and Chaperones

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Autophagy is used by organisms as a defense strategy to face environmental stress. This mechanism has been described as one of the most important intracellular pathways responsible for the degradation and recycling of proteins and organelles. It can act as a cell survival mechanism if the cellular damage is not too extensive or as a cell death mechanism if the damage/stress is irreversible; in the latter case, it can operate as an independent pathway or together with the apoptotic one. In this review, we discuss the autophagic process activated in several aquatic organisms exposed to different types of environmental stressors, focusing on the sea urchin embryo, a suitable system recently included into the guidelines for the use and interpretation of assays to monitor autophagy. After cadmium (Cd) exposure, a heavy metal recognized as an environmental toxicant, the sea urchin embryo is able to adopt different defense mechanisms, in a hierarchical way. Among these, autophagy is one of the main responses activated to preserve the developmental program. Finally, we discuss the interplay between autophagy and apoptosis in the sea urchin embryo, a temporal and functional choice that depends on the intensity of stress conditions.

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“…As such, they are critical for the growth and survival of ectothermic animals living in marine environments with variable temperatures (Mosser et al 1986;Hightower and Renfro 1988;Iwama et al 1998). The temperature range to which a fish species can adapt is dependent upon adaptive cellular functions and stress responses.…”

Section: Introductionmentioning

confidence: 99%

Stress Protein HSP70 in Fish

Yamashita¹,

Yabu²,

Ojima³

2010

Aqua-BioSci. Monogr. (ABSM)

128

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Stress proteins (heat-shock proteins, HSPs), which comprise an evolutionally wellconserved protein family, are induced in response to a variety of stress conditions and metabolic insults. When cells are subjected to sudden environmental changes, stress proteins are induced and play a central role in cellular homeostasis. A response to sudden adverse environmental changes is referred to as the heat-shock or stress response and is accompanied by a rapid increase in the synthesis of stress proteins. Given the importance of stress proteins in thermal adaptation at the cellular level, we have studied the expression, regulation, and protective functions of the members of the HSP70 stress protein family under normal and stress conditions in a variety of fish species. HSP70/heat-shock cognate protein-70 (HSC70) plays essential roles in the receptor complex formation and activation of Activin/Nodal/transforming growth factor-β and bone morphogenetic protein receptors and facilitates Nodal signaling. In addition, chaperone-mediated autophagy assisted by HSP70/heat-shock cognate (HSC)70 may be responsible for the stress responses in fish cells. HSP70 and HSC70 translocated into the lysosomes were found to accelerate protein degradation and catabolism under both stressed and normal conditions.

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Temperature ranges over which rainbow trout fibroblasts survive and synthesize heat‐shock proteins

Cited by 70 publications

References 38 publications

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

A new epithelial cell line, HBF from caudal fin of endangered yellow catfish, Horabagrus brachysoma (Gunther, 1864)

Autophagy as a defense strategy against stress: focus on Paracentrotus lividus sea urchin embryos exposed to cadmium

Stress Protein HSP70 in Fish

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