Temperature-Induced Ultradense PEG Polyelectrolyte Surface Grafting Provides Effective Long-Term Bioresistance against Mammalian Cells, Serum, and Whole Blood

Ogaki, Ryosuke; Andersen, Ole; Jensen, Grethe Vestergaard; Kolind, Kristian; Kraft, David C.; Pedersen, Jan Skov; Foss, Morten

doi:10.1021/bm301125g

Cited by 50 publications

(54 citation statements)

References 61 publications

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“…In addition, the RGD-functionalized copolymer was unable to strongly enhance the spreading of the cells compared to that observed on a pure PLL- g -PEG coating, perhaps because of low expression levels of RGD-specific integrins in the HEK293 cell line. Although the first issue – the adsorption of spreading-promoting secretion to the PLL backbone – could perhaps be dealt with by creating ultradense PEG-surfaces46, we could not overcome the second problem without genetic engineering. Therefore, we simply excluded the HEK293 cell line from further investigations in which the surface density of the RGD-motif was fine-tuned.…”

Section: Resultsmentioning

confidence: 99%

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Orgován

Péter

Bősze

et al. 2014

Sci Rep

105

192

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A novel high-throughput label-free resonant waveguide grating (RWG) imager biosensor, the Epic® BenchTop (BT), was utilized to determine the dependence of cell spreading kinetics on the average surface density (vRGD) of integrin ligand RGD-motifs. vRGD was tuned over four orders of magnitude by co-adsorbing the biologically inactive PLL-g-PEG and the RGD-functionalized PLL-g-PEG-RGD synthetic copolymers from their mixed solutions onto the sensor surface. Using highly adherent human cervical tumor (HeLa) cells as a model system, cell adhesion kinetic data of unprecedented quality were obtained. Spreading kinetics were fitted with the logistic equation to obtain the spreading rate constant (r) and the maximum biosensor response (Δλmax), which is assumed to be directly proportional to the maximum spread contact area (Amax). r was found to be independent of the surface density of integrin ligands. In contrast, Δλmax increased with increasing RGD surface density until saturation at high densities. Interpreting the latter behavior with a simple kinetic mass action model, a 2D dissociation constant of 1753 ± 243 μm−2 (corresponding to a 3D dissociation constant of ~30 μM) was obtained for the binding between RGD-specific integrins embedded in the cell membrane and PLL-g-PEG-RGD. All of these results were obtained completely noninvasively without using any labels.

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Section: Resultsmentioning

confidence: 99%

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Orgován

Péter

Bősze

et al. 2014

Sci Rep

105

192

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“…51,52 Prepared FCS/PCS/Gel/EGCG nanoparticles formed at pH 1.2 with pepsin resulted in a matrix structure, and EGCG released from the nanoparticles was 14.8 ± 1.1% at pH 1.2 ( Figure 4). CS, a biodegradable polymer, is known to be a weak base.…”

Section: ■ Discussionmentioning

confidence: 99%

Active Targeted Nanoparticles for Oral Administration of Gastric Cancer Therapy

et al. 2015

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Gastric carcinogenesis is a commonly diagnosed type of cancer and has a dismal prognosis because of the rate at which it aggressively spreads and because of the lack of effective therapies to stop its progression. This study evaluated a type of oral drug delivery system of a potential target-activated nanosizer comprising a fucose-conjugated chitosan and polyethylene glycol-conjugated chitosan complex with gelatin containing encapsulated green tea polyphenol extract epigallocatechin-3-gallate, allowing oral administration of the drug through a site-specific release in gastric cancer cells. The results demonstrated that the nanoparticles effectively reduced drug release within gastric acids and that a controlled epigallocatechin-3-gallate release inhibited gastric cancer cell growth, induced cell apoptosis, and reduced vascular endothelial growth factor protein expression. Furthermore, in vivo assay results indicated that the prepared epigallocatechin-3-gallate-loaded fucose-chitosan/polyethylene glycol-chitosan/gelatin nanoparticles significantly affected gastric tumor activity and reduced gastric and liver tissue inflammatory reaction in an orthotopic gastric tumor mouse model.

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“…In the case of poly(ethylene glycol) (PEG) derivatives the textile substrata were pre-treated with oxygen-plasma [23,24]. The coatings were analyzed for their antimicrobial property by the JIS L 1902:2002.…”

Section: Methodsmentioning

confidence: 99%

Rapid Assay to Assess Bacterial Adhesion on Textiles

et al. 2016

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Textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. Biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. To develop novel anti-adhesive textile coatings, a rapid, reliable, and quantitative high-throughput method to study microbial attachment to fabrics is required, however currently not available. Here, a fast and reliable 96-well plate-based screening method is developed. The quantification of bacterial adhesion is based on nucleic acid staining by SYTO9, with Pseudomonas aeruginosa and Staphylococcus aureus as the model microorganisms. Subsequently, 38 commercially available and novel coatings were evaluated for their anti-bacterial adhesion properties. A poly(l-lysine)-g-poly(ethylene glycol) coating on polyester textile substratum revealed an 80% reduction of bacterial adhesion. Both the coating itself and the anti-adhesive property were stable after 20 washing cycles, confirmed by X-ray analysis. The assay provides an efficient tool to rapidly screen for non-biocidal coatings reducing bacterial attachment.

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Temperature-Induced Ultradense PEG Polyelectrolyte Surface Grafting Provides Effective Long-Term Bioresistance against Mammalian Cells, Serum, and Whole Blood

Cited by 50 publications

References 61 publications

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Dependence of cancer cell adhesion kinetics on integrin ligand surface density measured by a high-throughput label-free resonant waveguide grating biosensor

Active Targeted Nanoparticles for Oral Administration of Gastric Cancer Therapy

Rapid Assay to Assess Bacterial Adhesion on Textiles

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