Temperature induced-masculinisation in the Nile tilapia causes rapid up-regulation of both dmrt1 and amh expressions

Poonlaphdecha, Srisupaph; Pepey, Elodie; Canonne, Marc; Verdal, Hugues de; Baroiller, Jean‐François; D'Cotta, Hélèna

doi:10.1016/j.ygcen.2013.06.007

Cited by 70 publications

(63 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was suggested as an important step required for the feminization of genetic male fish . On the other hand, there have been reports showing that inhibition of estrogen synthesis by aromatase inhibitors or androgen resulted in female to male sex reversal (Baron et al 2007, Poonlaphdecha et al 2013. The two opposite pathways are continuously antagonizing during development as demonstrated by the simultaneous expression of cyp19a1a and dmrt1 during sex reversal in the present study and by the simultaneous expression of cyp19a1a and cyp11b2 during secondary sex reversal .…”

Section: Discussionsupporting

confidence: 46%

“…However, this is opposite to the facts: i) it is easy to induce 100% female to male sex reversal but it is difficult to induce 100% male to female sex reversal and ii) secondary sex reversal of ovary into testis has been reported in medaka, zebrafish and tilapia (Poonlaphdecha et al 2013, Takatsu et al 2013, while there Figure 5 Expression profiles of methyltransferase genes in gonads. The expression levels of the maintenance methyltransferase gene dnmt1 were up-regulated significantly, while the expression levels of the de novo DNA methyltransferases genes dnmt3 and dnmt7 were down-regulated in the MTCE 2 -XX gonad but up-regulated in the MTCE 2 -XY gonad significantly compared with their respective control.…”

Section: Discussionmentioning

confidence: 84%

See 1 more Smart Citation

Simultaneous exposure to estrogen and androgen resulted in feminization and endocrine disruption

Chen¹,

Jiang²,

Feng³

et al. 2016

Journal of Endocrinology

View full text Add to dashboard Cite

Estrogen, which is synthesized earlier in females than androgen in males, is critical for sex determination in non-mammalian vertebrates. However, it remains unknown that what would happen to the gonadal phenotype if estrogen and androgen were administrated simultaneously. In this study, XY and XX tilapia fry were treated with the same dose of 17a-methyltestosterone (MT) and 17b-estradiol (E 2 ) alone and in combination from 0 to 30 days after hatching. Treatment of XY fish with E 2 resulted in male to female sex reversal, while treatment of XX fish with MT resulted in female to male sex reversal. In contrast, simultaneous treatment of XX and XY fish with MT and E 2 resulted in female, but with cyp11b2 and cyp19a1a co-expressed in the ovary. Serum 11-ketotestosteron level of the MT and E 2 simultaneously treated XX and XY female was similar to that of the XY control, while serum E 2 level of these two groups was similar to that of the XX control. Transcriptomic cluster analysis revealed that the MT and E 2 treated XX and XY gonads clustered into the same branch with the XX control. However a small fraction of genes, which showed disordered expression, may be associated with stress response. These results demonstrated that estrogen could maintain the female phenotype of XX fish and feminize XY fish even in the presence of androgen. Simultaneous treatment with estrogen and androgen up-regulated the endogenous estrogen and androgen synthesis, and resulted in disordered gene expression and endocrine disruption in tilapia.

show abstract

Section: Discussionsupporting

confidence: 46%

Section: Discussionmentioning

confidence: 84%

Simultaneous exposure to estrogen and androgen resulted in feminization and endocrine disruption

Chen¹,

Jiang²,

Feng³

et al. 2016

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…This difference represents just a fraction (8%) of the ∼15,000 DETs that were differentially expressed between untreated females (FCT) and males (MCT), which, in turn, account for a significant part of the estimated number of protein-coding genes (>25,000) in zebrafish. In fish, masculinization by elevated temperature usually involves down-regulation of profemale genes, such as cyp19a1a and foxl2, and up-regulation of promale genes, such as dmrt1 or amh (8)(9)(10)(11)(12)(13)(14). In accordance with this general pattern, the FHT2 females with a male-like gonadal transcriptome exhibited down-regulation of both key profemale genes such as cyp19a1a and vtg5 as well as downregulation of profemale pathways such as oocyte meiosis, progesterone-mediated oocyte maturation, canonical Wnt signaling (28,53), and Fanconi anemia (26).…”

Section: Discussionmentioning

confidence: 99%

“…1E and SI Appendix, Table S2). A large number of transcripts (14,783) were differentially expressed between these two groups ( Fig. 2A).…”

Section: Transcriptomic Analysis Of Adult Zebrafish Gonads Revealed Amentioning

confidence: 97%

“…The effects of temperature on gene expression during sex differentiation have been investigated in different teleost species, including the African catfish, Clarias gariepinus (8), European sea bass, Dicentrarchus labrax (9, 10), Japanese flounder, Paralichthys olivaceus (11), pejerrey, Odontesthes bonariensis (12), and Nile tilapia, Oreochromis niloticus (13,14). Regardless of the actual underlying sex-determining mechanism, a shared characteristic of all fish species in which temperature can alter sex ratios is that exposure to heat during early development upregulates the expression of genes related to testis differentiation with a concomitant down-regulation of genes related to ovarian differentiation, as assessed in several species (e.g., 9,13,[15][16][17].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes

Ribas

Liew

Díaz

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

110

136

View full text Add to dashboard Cite

Understanding environmental influences on sex ratios is important for the study of the evolution of sex-determining mechanisms and for evaluating the effects of global warming and chemical pollution. Fishes exhibit sexual plasticity, but the underlying mechanisms of environmental effects on their reproduction are unclear even in the well-established teleost research model, the zebrafish. Here we established the conditions to study the effects of elevated temperature on zebrafish sex. We showed that sex ratio response to elevated temperature is family-specific and typically leads to masculinization (female-to-male sex reversal), resulting in neomales. These results uncovered genotype-by-environment interactions that support a polygenic sex determination system in domesticated (laboratory) zebrafish. We found that some heat-treated fish had gene expression profiles similar to untreated controls of the same sex, indicating that they were resistant to thermal effects. Further, most neomales had gonadal transcriptomes similar to that of regular males. Strikingly, we discovered heat-treated females that displayed a normal ovarian phenotype but with a "male-like" gonadal transcriptome. Such major transcriptomic reprogramming with preserved organ structure has never been reported. Juveniles were also found to have a male-like transcriptome shortly after exposure to heat. These findings were validated by analyzing the expression of genes and signaling pathways associated with sex differentiation. Our results revealed a lasting thermal effect on zebrafish gonads, suggesting new avenues for detection of functional consequences of elevated temperature in natural fish populations in a global warming scenario.A major paradox in developmental biology is the lack of conservation of sex-determining mechanisms even in closely related taxa (1). Thus, in mammals and birds, sex is genetically canalized by a chromosomal sex determination (CSD) system of male (XX/XY in mammals) or female (ZW/ZZ in birds) heterogamety, driven by the action of a master sex-determining gene: sry in mammals and dmrt1 in birds (1). In contrast, fish sex can be very plastic, with the combination of genetic and environmental influences (2). In addition to fish species with CSD, there are species with polygenic sex determination (PSD), in which sex depends on the combined action of several promale and profemale genetic factors, and species with temperature-dependent sex determination (TSD) (3). Understanding how the environment influences sex ratios in vertebrates is of fundamental importance in the study of the evolution of sex-determining mechanisms (4, 5). It is also of practical relevance in evaluating the effects of climate change and chemical pollution (6, 7).The effects of temperature on gene expression during sex differentiation have been investigated in different teleost species, including the African catfish, Clarias gariepinus (8), European sea bass, Dicentrarchus labrax (9, 10), Japanese flounder, Paralichthys olivaceus (11), pejerrey, Odontesthes...

show abstract

Sex Control in Tilapias

Baroiller

D'Cotta

2018

Sex Control in Aquaculture

View full text Add to dashboard Cite

Temperature induced-masculinisation in the Nile tilapia causes rapid up-regulation of both dmrt1 and amh expressions

Cited by 70 publications

References 43 publications

Simultaneous exposure to estrogen and androgen resulted in feminization and endocrine disruption

Simultaneous exposure to estrogen and androgen resulted in feminization and endocrine disruption

Heat-induced masculinization in domesticated zebrafish is family-specific and yields a set of different gonadal transcriptomes

Sex Control in Tilapias

Contact Info

Product

Resources

About