2015
DOI: 10.1021/acs.analchem.5b01518
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Temperature Gradient Approach for Rapidly Assessing Sensor Binding Kinetics and Thermodynamics

Abstract: We report a highly resolved approach for quantitatively measuring the temperature dependence of molecular binding in a sensor format. The method is based on surface plasmon resonance (SPR) imaging measurements made across a spatial temperature gradient. Simultaneous recording of sensor response over the range of temperatures spanned by the gradient avoids many of the complications that arise in the analysis of SPR measurements where temperature is varied. In addition to simplifying quantitative analysis of bin… Show more

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Cited by 7 publications
(12 citation statements)
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“…It is possible to determine the temperature and salt concentration condition that causes DNA hybrids to denature by continuously increasing temperature or decreasing ionic concentration ( Figure 1b). In the literature, measurements of the melting of DNA hybrids have been performed as function of increasing temperature using readouts based on fluorescence [5][6][7] , electrochemistry [8][9][10] , surface plasmon resonance (SPR) 11,12 , surface enhanced Raman spectroscopy (SERS) 13 , and magnetoresistive sensors 14 . Several of these readouts have significant cross-sensitivities to temperature and salt concentration and thus require extra care.…”
Section: Introductionmentioning
confidence: 99%
“…It is possible to determine the temperature and salt concentration condition that causes DNA hybrids to denature by continuously increasing temperature or decreasing ionic concentration ( Figure 1b). In the literature, measurements of the melting of DNA hybrids have been performed as function of increasing temperature using readouts based on fluorescence [5][6][7] , electrochemistry [8][9][10] , surface plasmon resonance (SPR) 11,12 , surface enhanced Raman spectroscopy (SERS) 13 , and magnetoresistive sensors 14 . Several of these readouts have significant cross-sensitivities to temperature and salt concentration and thus require extra care.…”
Section: Introductionmentioning
confidence: 99%
“…Surface plasmon resonance (SPR) is a collective charge density oscillation that occurs at a metal-dielectric interface. As SPR is extremely sensitive to the temperature-dependent refractive index (RI) just above the metal film, it has extensive applications in temperature monitoring and sensing [ 20 , 21 , 22 ]. In work performed by Chiang et al, a variation as small as 0.027 K was distinguished at 632.8 nm [ 23 ].…”
Section: Introductionmentioning
confidence: 99%
“…22 Melting curves for surface-tethered DNA probes have also been measured using fluorescence 23 and surface plasmon reso-nance. 24,25 Compared to these methods, the GMR biosensors offer high sensitivity, no dependence on temperature and are insensitive to the sample matrix as virtually all biological material is nonmagnetic. 20,26 Using our technique, we analyzed melanoma cell lines with known genetic and epigenetic alterations.…”
mentioning
confidence: 99%
“…This avoids conventional assay condition optimization since the target-probe hybrids are exposed to continuously increasing stringency during melting curve measurement . Melting curves for surface-tethered DNA probes have also been measured using fluorescence and surface plasmon resonance. , Compared to these methods, the GMR biosensors offer high sensitivity, no dependence on temperature and are insensitive to the sample matrix as virtually all biological material is nonmagnetic. , …”
mentioning
confidence: 99%