Temperature Dependence of Unbinding Forces between Complementary DNA Strands

Schumakovitch, Irina; Grange, Wilfried; Strunz, Torsten; Bertoncini, Patricia; Güntherodt, H.‐J.; Hegner, Martin

doi:10.1016/s0006-3495(02)75416-7

Cited by 104 publications

(96 citation statements)

References 24 publications

(30 reference statements)

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“…In particular, Schumakovitch et al [1156] have studied the temperature dependence of unbinding forces between complementary DNA strands. Sattin et al [1146] determined the force Fig.…”

Section: Rupture Force Of Specific Interactionsmentioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,218

2,949

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“…In particular, Schumakovitch et al [1156] have studied the temperature dependence of unbinding forces between complementary DNA strands. Sattin et al [1146] determined the force Fig.…”

Section: Rupture Force Of Specific Interactionsmentioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,218

2,949

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“…5.2.1). Similar experiments have been carried out to investigate the kinetics of short DNA hairpins using SMF [161,162] or AFM [163,164,165,166,167] finding slower kinetics of unfolding/refolding depending on the length of the sequence as predicted by some theoretical models [133,168]. Mechanical unfolding of single RNA molecules through nanopores has been also proposed as a method to determine the secondary structure [169].…”

Section: Rnamentioning

confidence: 98%

Single-molecule experiments in biological physics: methods and applications

Ritort

2006

J. Phys.: Condens. Matter

389

463

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Abstract. I review single-molecule experiments (SME) in biological physics. Recent technological developments have provided the tools to design and build scientific instruments of high enough sensitivity and precision to manipulate and visualize individual molecules and measure microscopic forces. Using SME it is possible to: manipulate molecules one at a time and measure distributions describing molecular properties; characterize the kinetics of biomolecular reactions and; detect molecular intermediates. SME provide the additional information about thermodynamics and kinetics of biomolecular processes. This complements information obtained in traditional bulk assays. In SME it is also possible to measure small energies and detect large Brownian deviations in biomolecular reactions, thereby offering new methods and systems to scrutinize the basic foundations of statistical mechanics. This review is written at a very introductory level emphasizing the importance of SME to scientists interested in knowing the common playground of ideas and the interdisciplinary topics accessible by these techniques.

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“…Strunz et al [7,8] investigated the unbinding of DNA duplex of various lengths and found that the unbinding force depends on the loading rate and sequence length. It was also found that changing the pulling direction results different unbinding forces [11,13].…”

Section: Introductionmentioning

confidence: 99%

Statistical mechanics of DNA rupture: Theory and simulations

Nath

Modi

Mishra

et al. 2013

The Journal of Chemical Physics

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We study the effects of the shear force on the rupture mechanism on a double stranded DNA. Motivated by recent experiments, we perform the atomistic simulations with explicit solvent to obtain the distributions of extension in hydrogen and covalent bonds below the rupture force. We obtain a significant difference between the atomistic simulations and the existing results in the literature based on the coarse-grained models (theory and simulations). We discuss the possible reasons and improve the coarse-grained model by incorporating the consequences of semi-microscopic details of the nucleotides in its description. The distributions obtained by the modified model (simulations and theoretical) are qualitatively similar to the one obtained using atomistic simulations.

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Temperature Dependence of Unbinding Forces between Complementary DNA Strands

Cited by 104 publications

References 24 publications

Force measurements with the atomic force microscope: Technique, interpretation and applications

Force measurements with the atomic force microscope: Technique, interpretation and applications

Single-molecule experiments in biological physics: methods and applications

Statistical mechanics of DNA rupture: Theory and simulations

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