Temperature and Tissue Type Impact Development of Lucilia cuprina (Diptera: Calliphoridae) in Sri Lanka

Abstract

Lucilia cuprina (Wiedemann; Diptera: Calliphoridae) is a facultative ecto-parasitic fly, distributed throughout the temperate and subtropical regions of the world. This blow fly species is of medical, veterinary, and forensic importance due to it being used in maggot debridement therapy (MDT), a causative agent of myiasis, and a decomposer of vertebrate carrion. The current study examined the combined effects of temperature and tissue type on the development of L. cuprina. Specimens were reared on three tissue types; swine muscle, swine liver, and bovine muscle at 20°C, 25°C, 27°C, and 38°C. The optimum condition for L. cuprina development was for immatures reared on bovine muscle (287.4 h) followed by those reared on swine muscle (288.0 h) and swine liver (288.4 h) at 27°C. The minimum temperature threshold of L. cuprina was deduced to be 14°C. No significant differences in larval width and length over time were determined for the tissue type.

New Distribution Record for Lucilia cuprina (Diptera: Calliphoridae) in Indiana, United States

“…All L. sericata strains investigated exhibited similar minimum development times regardless of whether they originated from the United States ( Tarone et al 2011 ), Canada ( Anderson 2000 ), or Austria ( Grassberger and Reiter 2001 ) ( Table 2 ). Both Sri Lankan ( Bambaradeniya et al 2017 ) and Indian ( Bansode et al 2016 ) L. cuprina strains were similar in development time, though the U.S. strain ( Ash and Greenberg 1975b ) exhibited the longest development time of all strains. When L. sericata data are used to estimate a PMI MIN with L. cuprina specimens, percent error can range between 17.90% and 34.36% (mean = 26.71% ± 4.76%).…”

“…To date, only one developmental study is known to have been performed with a North American strain of L. cuprina ( Ash and Greenberg 1975b ), even though this species is encountered routinely in death investigations in the southern United States, particularly in southeast Texas ( Sanford 2017 ). In fact, only a handful of literature exists worldwide that investigates the developmental physiological requirements for this species ( Day and Wallman 2006 , Kotzé et al 2015 , Bansode et al 2016 , Bambaradeniya et al 2017 ). The three L. cuprina data sets compared here give relatively similar development and ADH values (<10.5% error) despite originating from three distant geographical regions.…”

“…A handful of studies have investigated the developmental requirements for North American strains of L. sericata ( Tarone and Foran 2006 , 2008 ; Gallagher et al 2010 ; Tarone et al 2011 ), but only one similar study exists for North American L. cuprina , though this was under the synonym P. pallescens ( Ash and Greenberg 1975b ). Development data for L. cuprina primarily exist for other regions, including Australia, South Africa, India, and Sri Lanka ( Day and Wallman 2006 , Kotzé et al 2015 , Bansode et al 2016 , Bambaradeniya et al 2017 ).…”

Terapia de desbridamiento larval: una revisión sistemática

“…Ambas poseen semejantes proteasas, con secreciones similares al utilizarlas en heridas. La terapia larval, mediante la excreción/secreción, puede estimular la cicatrización de las heridas degradando, por medio de sus enzimas, el tejido necrótico, remodelando las proteínas de la matriz extracelular, o estimulando la migración y proliferación de los fibroblastos (11)(12)(13) Las larvas de mosca son seres necrófagos, se alimentan descomponiendo y consumiendo el tejido necrótico enzimáticamente mediante un proceso de digestión extracorpórea. Esto se debe a que excretan/secretan una mezcla proteolítica, glicolítica, lipolítica y enzimas nucleasas sobre la superficie del tejido, lo cual causa la licuefacción y la digestión del tejido necrótico, siendo posteriormente ingerido (14).…”

Estimation of life expectancy and measurement of immature stages ofLucilia sericatafed on three kinds of diets

“…The mean size and number of larvae grown with beef were higher compared to the other media and beef has been introduced as the most suitable substrate to nurture larvae. Due to the odor and lack of sterilization, rearing larvae on animal tissue can be undesirable (6). In 1988, Mandeville introduced dry cat food with CSMA (special food for fly larvae) as a suitable diet to rear Lucillia sericata (7); however, Sherman rejected it because of the impossibility of sterilizing this diet (8).…”