Estimation of life expectancy and measurement of immature stages ofLucilia sericatafed on three kinds of diets

Abstract

Background and Objectives: Finding the best diet is very important to rear Lucilia sericata larvae for therapeutic purposes and prepare standard curves in forensic entomology. The aim of this study was to find the best diet for larvae in maggot therapy. Furthermore, this study was conducted to obtain a vertical life table and measure the length and width of immature stages of L. sericata for forensic entomology. Materials and Methods: Larvae of L. sericata (Karaj strain) were used to evaluate diets. The tests were carried out in three replicates of 100 eggs for each diet including chicken liver, blood agar, and fish food at the same time. Independent t-test, ANOVA, and Tukey’s post-hoc tests were used to compare the mean length and width of larvae between different groups. A P-value of less than 0.05 was considered significance level. Results: In contrast to the first and the second larval instars, there was a significant difference in the mean length and width of the third instar larvae (P<0.001). At the third instar larval stage, those larvae that had been fed on chicken liver were significantly larger than the others. There were also no significant differences between life expectancy, overall survivorship rate, and force of mortality of larval groups fed on three different diets. Conclusions: Chicken liver is an advisable diet for nurturing larvae and plotting standard curves in forensic entomology. For rearing the first and the second larval instars in sterile conditions, blood agar and fish food can serve as suitable diets.

A Parallel Randomized Clinical Trial for Comparison of Two Methods of Maggot Therapy, Free-Range Larvae and Larval-bag, in Diabetic Ulcer (Wagner 2)

“…The eggs were washed with 70% ethanol solution and chloramine for disinfecting. 19 The disinfected eggs were transferred to sterile filter to flask and left 1 night in clean area hatching and preparing sterile first instar larvae for use as free-range larvae. For preparing suitable larvae for larval-bag, the previously disinfected larvae of first instars were put on sterile medium to be grown to young disinfected second instar and transferred to the sterile bags which are made of polyvinyl alcohol.…”

Exploring the Diversity and Abundance of Necrophagous Flies in Tabriz City, Northwest of Iran: Implications for Medical and Health Importance

“…These traps were placed early morning and picked up late evening. Furthermore, the larvae stages of ies were collected from chicken liver placed in open plastic in the environment (Dehghan et al 2020) and adults attracted to bait were caught by hand net. The trapped adult ies and larvae were transferred to the laboratory and adults were mounted.…”