Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

Pal, Anusuya; Gope, Amalesh; Iannacchione, Germano S.

doi:10.3390/biom11020231

Cited by 16 publications

(24 citation statements)

References 35 publications

(74 reference statements)

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“…However, it should be noted that large variations in the evaporation temperature can affect the final width of the peripheral ring [58]. Recently, Pal et al [46] showed that the width of the peripheral ring changes as the substrate temperature increases from 25 °C to 55 °C for dried deposits of BSA drops. However, in our case, no significant changes of the ring width were observed.…”

Section: Glassy Peripheral Protein Ring and Crack Formationmentioning

confidence: 99%

“…Iqbal et al [45] probed the effect of dilution and substrate wettability on the evaporative behaviour and desiccation patterns of blood drops. Additionally, Pal et al [46] investigated the drying dynamics and morphological features of blood and aqueous BSA drops at various initial concentrations and substrate temperatures. Very recently, Carreon et al [47] examined the effect of substrate temperature on the drying patterns of protein mixtures and salt-protein mixtures, to investigate the most suitable drying temperature for efficient diagnosis.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Substrate Temperature on the Evaporative Behaviour and Desiccation Patterns of Foetal Bovine Serum Drops

Efstratiou

Christy

Bonn

et al. 2021

Colloids and Interfaces

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The drying of bio-fluid drops results in the formation of complex patterns, which are morphologically and topographically affected by environmental conditions including temperature. We examine the effect of substrate temperatures between 20 °C and 40 °C, on the evaporative dynamics and dried deposits of foetal bovine serum (FBS) drops. The deposits consist of four zones: a peripheral protein ring, a zone of protein structures, a protein gel, and a central crystalline zone. We investigate the link between the evaporative behaviour, final deposit volume, and cracking. Drops dried at higher substrate temperatures in the range of 20 °C to 35 °C produce deposits of lower final volume. We attribute this to a lower water content and a more brittle gel in the deposits formed at higher temperatures. However, the average deposit volume is higher for drops dried at 40 °C compared to drops dried at 35 °C, indicating protein denaturation. Focusing on the protein ring, we show that the ring volume decreases with increasing temperature from 20 °C to 35 °C, whereas the number of cracks increases due to faster water evaporation. Interestingly, for deposits of drops dried at 40 °C, the ring volume increases, but the number of cracks also increases, suggesting an interplay between water evaporation and increasing strain in the deposits due to protein denaturation.

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Section: Glassy Peripheral Protein Ring and Crack Formationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of Substrate Temperature on the Evaporative Behaviour and Desiccation Patterns of Foetal Bovine Serum Drops

Efstratiou

Christy

Bonn

et al. 2021

Colloids and Interfaces

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“…We examined the time-dependent changes in the concentration and quality metrics of total RNA at two different temperatures. We hypothesized that the concentration of total RNA in the blood samples will decrease with a longer TSD due to the activity of exogenous RNases [41,42] and the deformation of white blood cells as the blood dries [11,12]. We predicted that quality metrics, such as the RNA Integrity Number equivalent (RINe), the DV200 (percentage of RNA fragments greater than 200 nucleotides), and the 28S/18S rRNA peak area ratio, will decrease over time due to fragmentation of larger RNA fragments and a corresponding increase of smaller RNA fragments [42].…”

Section: Introductionmentioning

confidence: 99%

“…These tools do not provide TSD estimates of blood, requiring additional analytical approaches to be investigated. A bloodstain ages in a series of complex processes influenced by many factors, ranging from its formation on a surface [10][11][12][13] to the degradation it undergoes over time [14][15][16][17]. Previous studies have developed chemometric [2,14,15,18] and genetic methods [3,[19][20][21] that estimate the TSD of bloodstains in controlled laboratory conditions.…”

Section: Introductionmentioning

confidence: 99%

Using total RNA quality metrics for time since deposition estimates in degrading bloodstains

Elliott

Stotesbury

Shafer

2021

Preprint

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Determining the age, or time since deposition (TSD), of bloodstains would provide forensic scientists with critical information regarding the timeline of the events of bloodshed during a crime. The physicochemical changes that occur to major biomolecules as a bloodstain dries can be used to estimate the TSD of bloodstains. For example, high-resolution automated gel electrophoresis can be used to quantify the timewise degradation of DNA present in bloodstains. Our study aims to analyze and quantify the timewise degradation trends found in total RNA from bloodstains, expanding the scope of the TSD research which has previously explored DNA and targeted mRNA molecules. Fifty bloodstains were stored in plastic microcentrifuge tubes at room temperature and tested over 10 different timepoints spanning one week. A total of eight RNA metrics were visually assessed and quantified using linear regression. RNA Integrity Number equivalent (RINe), total RNA concentration, and 28S/18S rRNA peak area ratios were retained for further analyses based on their relationship with time and limited correlations. RINe and total RNA concentration both exhibited negative trends over time, highlighting a decrease in quality and quantity. RINe was the RNA metric that demonstrated the greatest association with time (R2 = 0.696). Generalized linear mixed-effects models including donor (biological replicate) as a random effect increased the fit for all RNA metrics to varying degrees, but no significant differences were found between biological replicates for the RINe metric. Our results illustrated the presence of a significant decrease in the retained RNA metrics after 24 hours, suggesting that this method could be used to reliably differentiate day-old bloodstains from older bloodstains. Future work should focus on recreating this study in different environmental conditions, including testing on a variety of substrates.

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“…The time factor is, therefore, as important as the other factors considered here. For example, the quality of many biomedical liquids decreases with time [1][2][3], which changes their surface chemistry properties. Therefore, effective measurement of physical-chemical properties of a liquid utilizing the least amount of liquid and a fast-paced technique, is in considerable demand in the modern era.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Viscosity Dependence of the Critical Meniscus Height with Optical Fiber Sensors

Binetti

Simpson

Alwis

2021

Sensors

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Conventional means of data extraction using optical fiber interrogators are not adequate for fast-paced detection of a target parameter. In this instance, the relationship between the critical meniscus heights (CMH) of several liquids to the extraction speed of a rod submerged in them, have been analyzed. A limitation of a previous interrogator used for the purpose had been light absorption by the liquid due to the used bandwidth of the readily-available light source, i.e., C-band. The newly proposed technique addresses this limitation by utilizing a broadband light source instead, with a Si-photodetector and an Arduino. In addition, the Arduino is capable of extracting data at a relatively faster rate with respect to the conventional optical interrogator. The use of a different operational wavelength (850 nm instead of 1550 nm) increased the r2 and the sensitivity of the sensor. The new setup can measure surface chemistry properties, with the advantage of being comparatively cheaper than the conventionally available interrogator units, thereby providing a suitable alternative to conventional measurement techniques of liquid surface properties, while reducing material waste, i.e., in terms of the required volume for detection of a target parameter, through the use of optical fiber.

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Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

Cited by 16 publications

References 35 publications

The Effect of Substrate Temperature on the Evaporative Behaviour and Desiccation Patterns of Foetal Bovine Serum Drops

The Effect of Substrate Temperature on the Evaporative Behaviour and Desiccation Patterns of Foetal Bovine Serum Drops

Using total RNA quality metrics for time since deposition estimates in degrading bloodstains

Evaluation of Viscosity Dependence of the Critical Meniscus Height with Optical Fiber Sensors

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