Temperature and cholesterol composition-dependent behavior of 1-myristoyl-2-[12-[(5-dimethylamino-1-naphthalenesulfonyl)amino]dodecanoyl]-sn-glycero-3-phosphocholine in 1,2-dimyristoyl-sn-glycero-3-phosphocholine membranes

A new surfactant-fluorescence probe for detecting shape transitions in self-assembled systems

“…This problem may be expected to be solved by the use of fluorophorelabeled amphiphiles, since this kind of fluorescence probe as part of the surfactant can take part in the formation of aggregates and provide more reliable information. Based on the considerations mentioned above, some fluorophore-labeled amphiphiles have been used to detect the correlative physical properties of various aggregates [20][21][22][23][24][25][26][27][28][29][30]. However, most of these studies focus on micelle [20][21][22][23][24] or membrane [25][26][27][28][29] systems.…”

Measuring Raft Size as a Function of Membrane Composition in PC-Based Systems: Part 1 − Binary Systems

“…If, as we state, DAN-PC is truly excluded from the l o phase, then as long as there is some l d in which it is able to reside, the blue shift would be expected to occur suddenly, resulting in a step change in the DAN-PC emission maximum wavelength rather than a gradual decrease as was seen. However, it has been well established by our group that the DANSYL moiety, when it is attached to the chain of a phospholipid molecule, resides in different positions within the membrane depending on the local cholesterol composition . As the membrane becomes more ordered with more cholesterol, the DANSYL moiety is forced further down into the membrane.…”

Phospholipid-stabilized microbubbles: Influence of shell chemistry on cavitation threshold and binding to giant uni-lamellar vesicles

“…We favor phospholipids, which are used in commercial formulations [1,38], are commonplace in academic formulations [39][40][41][42], and -as they are components of native cell membranes -are inherently biocompatible. Moreover, phospholipid vesicles serve as cell membrane mimics, and study of phospholipid bilayers and associated phase behavior is a mature field within the biophysics community [43][44][45][46][47][48]. We are not the first to appreciate this, as others have already demonstrated analogies between phospholipid bilayer systems and bubble phospholipid monolayers; particularly exciting among these are systems demonstrating multi-phase coexistence and examinations of cholesterol [49][50][51].…”