Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines

Kabaso, Doron; Lokar, Maruša; Kralj‐Iglič, Veronika; Veranič, Peter; Iglic, Alessandro

doi:10.2147/ijn.s16982

Cited by 45 publications

(53 citation statements)

References 35 publications

(77 reference statements)

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“…15,42,43,51 The basic equations of motion of the membrane contour and the time dependence of the nonhomogeneous lateral density of integrin molecules are derived in Appendix A.…”

Section: Dynamic Modelmentioning

confidence: 99%

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Adhesion of osteoblasts to a nanorough titanium implant surface

Gongadze¹,

Kabaso²,

Bauer³

et al. 2011

IJN

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This work considers the adhesion of cells to a nanorough titanium implant surface with sharp edges. The basic assumption was that the attraction between the negatively charged titanium surface and a negatively charged osteoblast is mediated by charged proteins with a distinctive quadrupolar internal charge distribution. Similarly, cation-mediated attraction between fibronectin molecules and the titanium surface is expected to be more efficient for a high surface charge density, resulting in facilitated integrin mediated osteoblast adhesion. We suggest that osteoblasts are most strongly bound along the sharp convex edges or spikes of nanorough titanium surfaces where the magnitude of the negative surface charge density is the highest. It is therefore plausible that nanorough regions of titanium surfaces with sharp edges and spikes promote the adhesion of osteoblasts.

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“…15,42,43,51 The basic equations of motion of the membrane contour and the time dependence of the nonhomogeneous lateral density of integrin molecules are derived in Appendix A.…”

Section: Dynamic Modelmentioning

confidence: 99%

“…In particular, it was shown that the time scale for the coalescence of membrane protrusions and membrane growth is shorter, and as a result, more membrane regions adhered to the titanium surface, which is due to the increased instability of the model system. 15,42,43 …”

Section: Dovepressmentioning

confidence: 99%

Adhesion of osteoblasts to a nanorough titanium implant surface

Gongadze¹,

Kabaso²,

Bauer³

et al. 2011

IJN

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“…As the ICNs are supposed to represent an important communication system enabling metastatic cancer cells to explore their surroundings, 7 their reduction by cholesterol removal might represent a new target for anticancer therapy.…”

Section: Discussionmentioning

confidence: 99%

“…[1][2][3][4][5][6][7] Previously, it has been shown that ICNs are stabilized not only by the inner cytoskeleton but also by the membrane composition. [8][9][10] Yet, due to their fragility, it is impossible to separate the ICNs from the rest of the cell, and therefore their membrane composition remains unknown.…”

Section: Introductionmentioning

confidence: 99%

The role of cholesterol-sphingomyelin membrane nanodomains in the stability of intercellular membrane nanotubes

Lokar

Kabaso²,

Resnik³

et al. 2012

IJN

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Intercellular membrane nanotubes (ICNs) are highly curved tubular structures that connect neighboring cells. The stability of these structures depends on the inner cytoskeleton and the cell membrane composition. Yet, due to the difficulty in the extraction of ICNs, the cell membrane composition remains elusive. In the present study, a raft marker, ostreolysin, revealed the enrichment of cholesterol-sphingomyelin membrane nanodomains along ICNs in a T24 (malignant) urothelial cancer cell line. Cholesterol depletion, due to the addition of methyl-β-cyclodextrin, caused the dispersion of cholesterol-sphingomyelin membrane nanodomains and the retraction of ICNs. The depletion of cholesterol also led to cytoskeleton reorganization and to formation of actin stress fibers. Live cell imaging data revealed the possible functional coupling between the change from polygonal to spherical shape, cell separation, and the disconnection of ICNs. The ICN was modeled as an axisymmetric tubular structure, enabling us to investigate the effects of cholesterol content on the ICN curvature. The removal of cholesterol was predicted to reduce the positive spontaneous curvature of the remaining membrane components, increasing their curvature mismatch with the tube curvature. The mechanisms by which the increased curvature mismatch could contribute to the disconnection of ICNs are discussed.

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“…Yet, the underlying dynamics of membrane bending concomitant to the aggregation of negatively charged lipids is poorly understood. In previous theoretical studies, we used a continuum model to describe how membrane complexes (either proteins or lipids) that have spontaneous curvature could initiate the formation of membrane protrusions [12][13][14][15]. The coupling of the spontaneous curvature of membrane complexes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Exploring the binding dynamics of BAR proteins

Kabaso¹,

Gongadze²,

Jorgačevski³

et al. 2011

Cellular and Molecular Biology Letters

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Abstract:We used a continuum model based on the Helfrich free energy to investigate the binding dynamics of a lipid bilayer to a BAR domain surface of a crescent-like shape of positive (e.g. I-BAR shape) or negative (e.g. F-BAR shape) intrinsic curvature. According to structural data, it has been suggested that negatively charged membrane lipids are bound to positively charged amino acids at the binding interface of BAR proteins, contributing a negative binding energy to the system free energy. In addition, the cone-like shape of negatively charged lipids on the inner side of a cell membrane might contribute a positive intrinsic curvature, facilitating the initial bending towards the crescent-like shape of the BAR domain. In the present study, we hypothesize that in the limit of a rigid BAR domain shape, the negative binding energy and the coupling between the intrinsic curvature of negatively charged lipids and the membrane curvature drive the bending of the membrane. To estimate the binding energy, the electric potential at the charged surface of a BAR domain was calculated using the Langevin-Bikerman equation. Results of numerical simulations reveal that the binding energy is important for the initial instability (i.e. bending of a membrane), while the coupling between the intrinsic shapes of lipids and membrane curvature could be crucial for the curvature-dependent aggregation of negatively charged lipids near the surface of the BAR domain. In the discussion,

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Temperature and cholera toxin B are factors that influence formation of membrane nanotubes in RT4 and T24 urothelial cancer cell lines

Cited by 45 publications

References 35 publications

Adhesion of osteoblasts to a nanorough titanium implant surface

Adhesion of osteoblasts to a nanorough titanium implant surface

The role of cholesterol-sphingomyelin membrane nanodomains in the stability of intercellular membrane nanotubes

Exploring the binding dynamics of BAR proteins

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