TEM study of calcium phosphate precipitation on HA/TCP ceramics

Leng, Yang; Chen, Jiyong; Qu, Shuxin

doi:10.1016/s0142-9612(03)00036-x

Cited by 91 publications

(63 citation statements)

References 11 publications

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“…Numerous studies report the formation of an apatite layer between bone and implant, up to 1000 mm in thickness [36,[47][48][49], commonly accepted to occur through a dissolution-reprecipitation process [47]. Composition and orientation of this layer is debated to date [50][51][52][53][54][55]. In our recent work, we employed ET for the study of the first biomaterial-bone interface in nanoscale three-dimensional resolution [36].…”

Section: (A) Hydroxyapatite-bone Interfacementioning

confidence: 99%

High-resolution three-dimensional probes of biomaterials and their interfaces

Grandfield

Palmquist

Engqvist

2012

Phil. Trans. R. Soc. A.

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Interfacial relationships between biomaterials and tissues strongly influence the success of implant materials and their long-term functionality. Owing to the inhomogeneity of biological tissues at an interface, in particular bone tissue, two-dimensional images often lack detail on the interfacial morphological complexity. Furthermore, the increasing use of nanotechnology in the design and production of biomaterials demands characterization techniques on a similar length scale. Electron tomography (ET) can meet these challenges by enabling high-resolution three-dimensional imaging of biomaterial interfaces. In this article, we review the fundamentals of ET and highlight its recent applications in probing the three-dimensional structure of bioceramics and their interfaces, with particular focus on the hydroxyapatite-bone interface, titanium dioxide-bone interface and a mesoporous titania coating for controlled drug release.

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Section: (A) Hydroxyapatite-bone Interfacementioning

confidence: 99%

High-resolution three-dimensional probes of biomaterials and their interfaces

Grandfield

Palmquist

Engqvist

2012

Phil. Trans. R. Soc. A.

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“…3,[10][11][12] The morphology and structure of apatites, in turn, dictate the biomechanical and biochemical properties. 2,3,5,[13][14][15][16] However, few ultrastructurallevel studies (from 0.1 to 100 nm) on apatite films deposited from SBF have been conducted, and most transmission electron microscopy (TEM) studies have been performed on apatite powders or precipitates processed by methods that are subject to limitations. For example, Lu and Leng 13 prepared TEM specimens by extracting the deposited film by an ultrasonic vibration method, and Chou et al 3 prepared specimens by scraping off the deposited film, suspending apatite precipitates in isopropanol, and picking them up on Cu grids.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural analyses of nanoscale apatite biomimetically grown on organic template

et al. 2008

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The ultrastructure of nanoscale apatite biomimetically formed on an organic template from a supersaturated mineralizing solution was studied to examine the morphological and crystalline arrangement of mineral apatites. Needle-shaped apatite crystal plates with a size distribution of ~100 to ~1000 nm and the long axis parallel to the c axis ([002]) were randomly distributed in the mineral films. Between these randomly distributed needle-shaped apatite crystals, amorphous phases and apatite crystals (~20-40 nm) with the normal of the grains quasi-perpendicular to the c axis were observed. These observations suggest that the apatite film is an interwoven structure of amorphous phases and apatite crystals with various orientations. The mechanisms underlying the shape of the crystalline apatite plate and aggregated apatite nodules are discussed from an energy-barrier point of view. The plate or needle-shaped apatite is favored in single-crystalline form, whereas the granular nodules are favored in the polycrystalline apatite aggregate. The similarity in shape in both singlecrystalline needle-shaped apatite and polycrystalline granular apatite over a wide range of sizes is explained by the principle of similitude, in which the growth and shape are determined by the forces acting upon the surface area and the volume.

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“…The most two visible rings could be regarded as octacalcium phosphate (OCP) rings of (0 0 2) and (4 0 2) or hydroxyapatite (HA) rings of (0 0 2) and (2 1 1), even though it is considered controversial to perform phase identification from TEM ring patterns. 23) Quantitative EDX analysis of the newly formed spherulite layer on TiO 2 and P-200 surfaces showed that the relative amount of Ca/P was 1.25 and 1.54, respectively. Nevertheless, the Ca/P atomic ratio in both the samples (TiO 2 and P-200) were much lower than that of bone apatite (Ca/P = 1.65) and stoichiometric hydroxyapatite [Ca 10 (PO 4 ) 6 (OH) 2 ] (Ca/P = 1.67).…”

Section: Characterization Of Microspheresmentioning

confidence: 99%

Preparation and in vitro apatite-forming ability of porous and non-porous titania microspheres

Miyazaki

Kawashita

2013

J. Ceram. Soc. Japan

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Porous and non-porous titania microspheres with the anatase or rutile phase were successively prepared by the solgel process followed by heat treatment at various temperatures. The pore size of the prepared microspheres was effectively controlled by incorporating silica nanoparticles of different diameters. The apatite-forming ability of the microspheres was investigated in a simulated body fluid, with ion concentrations nearly equal to those of human blood plasma. Results indicated that the titania microspheres with either anatase or rutile structure induced the formation of calcium phosphate compounds (CaPs) on the microsphere surface. The deposition of CaPs was more pronounced on the TiO 2 microspheres calcined at 600°C (anatase structure) and 800°C (rutile structure), compared to that calcined at 500°C (anatase structure). Additionally, anatase microspheres with smooth surface and low specific surface area favored the formation of CaPs, compared to porous microspheres. This indicates that nanoscale pores do not essentially favor apatite formation.

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TEM study of calcium phosphate precipitation on HA/TCP ceramics

Cited by 91 publications

References 11 publications

High-resolution three-dimensional probes of biomaterials and their interfaces

High-resolution three-dimensional probes of biomaterials and their interfaces

Ultrastructural analyses of nanoscale apatite biomimetically grown on organic template

Preparation and in vitro apatite-forming ability of porous and non-porous titania microspheres

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