Telomeric DNA quantity, DNA damage, and heat shock protein gene expression as physiological stress markers in chickens

Sohn, Sea Hwan; Subramani, Vinod Kumar; Moon, Yeonsil; Jang, In-Surk

doi:10.3382/ps.2011-01904

Cited by 46 publications

(56 citation statements)

References 43 publications

(55 reference statements)

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“…가금류에 있어 스트레스 반응 정도 측정에 생리적 스 트레스 지표로 가장 널리 이용되고 있는 것이 부신피질호르 몬의 일종인 코르티코스테론(corticosterone)의 수준과 heterophil:lymphocyte의 비율(H/L ratio)이다 (Beuving and Vonder, 1978;Gross and Siegel, 1983;Davis and Sipoes, 1987;Gross, 1989;Maxwell, 1993;Zulkifli et al, 1995;Fraisse and Cockrem, 2006;Rimoldi et al, 2015). 그러나 이러한 생화학적 지 표는 측정 변이나 개체 변이가 큼에 따라 최근에는 스트레 스 관련 유전자 표지들이 스트레스 예측 지표로 소개되고 있 는데, 텔로미어 함유율이나 DNA 손상율 및 열 스트레스 단 백질 발현율 등이 새롭게 제시되고 있다 (Kregel, 2002;Gornati et al, 2004;Chen et al, 2007;Beloor et al, 2010;Sohn et al, 2012). 텔로미어는 진핵세포 염색체 말단부의 단순 반복 염기서열로 노화가 진행됨에 따라 이의 길이가 짧아진다 (Cottliar and Slavutsky, 2001).…”

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“…또한 텔로미어 길이의 감축 정도는 유전적 요인뿐만 아니라, 환경적 요인에 의해 많은 영 향을 받는 것으로 보고되고 있다 (Meeker and Coffey, 1997;Richter and Proctor, 2007;Sohn and Subramani, 2014). 특히, 닭에 있어 환경 스트레스 중 밀사 스트레스는 텔로미어 유실 을 급격히 촉진시킨다고 하였다 (Beloor et al, 2010;Sohn et al, 2012;. 열 스트레스 단백질(Heat Shock Proteins; HSPs) 유전자의 발현율 또한 중요한 스트레스 반 응 표지로 알려져 있다.…”

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Comparison of Stress Response in Diallel Crossed Korean Domestic Chicken Breeds

Cho¹,

Park²,

Choi³

et al. 2016

Korean Journal of Poultry Science

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To establish a new synthetic Korean meat chicken breed, we tested 5×5 diallel cross mating experiment with domestic chicken breeds. Comparing stress responses among diallel crossed chicken breeds, we analyzed telomere length, DNA damage and expressions of heat shock protein genes (HSPs) as the markers of the stress response. The telomere length was measured by quantitative fluorescence in situ hybridization on the nuclei of lymphocytes. The expression levels of HSP-70, HSP-90α and HSP-90β genes were analyzed by quantitative real-time polymerase chain reaction in lymphocytes. The DNA damage rate of lymphocytes was quantified by the comet assay known as the single cell gel electrophoresis. In results, there were significant differences in the values of the stress markers such as telomere length, HSPs and DNA damage rate, and also were significant differences in viabilities and body weights among the 5×5 diallel crossed chicken breeds. The telomere shortening rate, expression values of HSPs and DNA damage rate were significant low in W and Y crossed chickens compare to the others, but GG pure breed showed the highest values in the 25 crossed chickens. Estimating correlation coefficient, the survival rate positively correlated to telomere length, but negatively correlated to the expression levels of HSP-70, HSP-90α, HSP-90β genes and to the value of % DNA in tail as DNA damage rate. The expression levels of HSP-70, HSP-90α and HSP-90β genes of dead chickens had significantly higher than those of survival chickens. According to the results on the stress marker analysis, it would be considered that the crossed breeds had more stress resistant than the pure breeds, and the crossed chickens with a light strain such as W or Y were relatively resistant to stress, but the crossed chickens with a heavy strain such as G, H, F were susceptible to stress.

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Comparison of Stress Response in Diallel Crossed Korean Domestic Chicken Breeds

Cho¹,

Park²,

Choi³

et al. 2016

Korean Journal of Poultry Science

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“…이밖에도 IL-1β, IL-6, IL-18 및 IFN-γ와 같은 염증반 응 촉진 사이토카인(pro-inflammantory cytokines) 유전자 발 현율, 열스트레스 단백질(heat shock proteins; HSPs) 유전자 발현율, 텔로미어 함유율 및 DNA 손상율 등이 또 다른 개체 의 스트레스 반응 정도를 나타내는 생리적 표지로 알려져 있 다 (Kregel, 2002;Gornati et al, 2004;Chen et al, 2007;Beloor et al, 2010;Soleimani et al, 2011;Sohn et al, 2012;. 이들 중 염색체 말단부를 지칭하는 텔로미어는 대 표적 노화 표지로 연령이 증가함에 따라 길이가 줄어들게 되 는데, 이의 감축 정도는 유전적, 환경적 요인에 따라 많은 차이를 나타낸다 (Meeker and Coffey, 1997;Cottliar and Slavutsky, 2001;Richter and Proctor, 2007;Sohn and Subramani, 2014).…”

unclassified

“…닭에 있어 이러한 급만성 열 스트레스는 HSPs 유 전자의 발현 증가와 더불어 산란계 및 육계의 생산능력 저 하를 유발하고, 체내 면역력을 약화시켜 질병의 감염빈도를 증가시킨다 (Reece et al, 1972;Muiruri and Harrison, 1991;Njoya and Picard, 1994;Woelfel et al, 2002;Lin et al, 2004;Mashaly et al, 2004;Al-Fataftah and Abu-Dieyeh, 2007;Song et al 2012;Zulkifli et al, 2014 (Lee and Steinert, 1995;Ryu et al, 1997;Lew et al, 2004). Comet assay에 의한 comet score는 산화적 스트레스 시험이나 유전독성 시험에서 DNA 손상정도를 알 기 위해 널리 이용되는 지표로서, 닭의 스트레스 반응 정도 측정에도 이러한 표지가 유용하게 이용되고 있다 (Baos et al, 2006;Frankic et al, 2006;Sohn et al, 2012;Cho et al, 2016 (Bobek et al, 1980;Garriga et al, 2006;Star et al, 2008a;Quinterio-Filho et al, 2010;Kamboh et al, 2013).…”

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The Relationship of the Expressions of Stress-related Markers and Their Production Performances in Korean Domestic Chicken Breed

Park¹,

Cho²,

Choi³

et al. 2016

Korean Journal of Poultry Science

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This study was conducted to verify the relationships between the expression values of stress-related markers and their production performances in 25 strains of Korean domestic chicken breeds. For stress response markers, the amount of telomeric DNA; expression levels of heat shock protein (HSP)-70, HSP-90α, and HSP-90β; and comet scores were analyzed. Production performances were measured by the survival rate, body weights, days at first egg laying, egg weight and hen housed egg production. The results showed that the production traits and values of stress-related markers showed significant differences between strains. In general, the stress response of pure bred chickens with heavy weights was relatively high, while that of hybrid chickens with light weights was relatively low. The correlation coefficients between telomere contents and body weights showed that there were weak negative relationships. However, the correlations of telomere content with the survival rate and egg production were weakly positive after 20 weeks old. The expression levels of HSP genes and DNA damage rate (comet scores) were positively correlated to body weight, but were negatively correlated to the survival rate and egg production. The results implied that increasing body weight was associated with increasing HSPs expression and the DNA damage rate was associated with decreasing telomere content. In addition, increasing HSPs expression and the DNA damage rate decreased the survival rate and egg production, but the relationships with the telomere content was the reverse. Correlations among the stress-related markers showed that there were significant correlation coefficients between all of the marker values. HSPs expression was negatively correlated to the telomere content, while it was positively correlated to the DNA damage rate. There was a highly negative correlation between the telomere content and DNA damage rate. In conclusion, increasing the HSP values and DNA damage rate can promote telomere reduction, which led to a decrease in disease resistance and robustness of the chicken. Thus, increasing the stress response was verified to adversely affect the laying performance and viability of chickens.

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“…There is no doubt that DNA lesions are accumulating with age (Takubo et al, 2010). However, the rate of DNA lesions accumulation could vary widely in different cell types (Castro et al, 2012;Sohn et al, 2012) and differ between species (Meyer et al, 2007;Hunter et al, 2010). The amount of DNA damage also significantly depends on the type of genome: nuclear or mitochondrial (Wang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Age-Dependent Variation of Telomere Length and DNA Damage in Chicken

Makarenko

Усатов

Getmantseva

et al. 2017

OnLine Journal of Biological Sciences

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Abstract:Birds are unique models for molecular studies on aging processes and cellular senescence because of their slow rates of aging and comparatively long life spans among homoeothermic animals. The study aimed to examine the relative telomere length and amount of nuclear, mitochondrial DNA lesions in hens of different ages and to find out the correlation between these biomarkers of aging. The study was carried out on hens of crossbreed «High Sex Brown» of different age groups -1, 75, 225, 330, 450 days old. The measurements of telomeres and DNA damage were done in red cells of hens using quantitative PCR technique. We found out that the amount of telomeric DNA reduced significantly with age. Telomere length ratio to control group (1 day old) made up: 0.88±0.08 for 75 days old, 0.73±0.07 for 225 days old, 0.66±0.1 for 330-days old and 0.58±0.12 for 450 days old. The amount of DNA lesions in both genomes had great, age-dependent increase. The relative quantity of lesions per 10 kb detected in the nuclear DNA were as follows: 0.17±0.19 for 75 days old, 0.66±0.21 for 225 days old, 1.02±0.33 for 330-days old and 1.48±0.45 for 450 days old and the relative quantity of lesions in mtDNA were as follows: 0.11±0.06 for 75 days old, 0.84±0.23 for 225 days old, 1.18±0.3 for 330-days old and 1.49±0.35 for 450 days old. The results obtained are of fundamental interest for aging processes, as well as could have an economic value for poultry farming.

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Telomeric DNA quantity, DNA damage, and heat shock protein gene expression as physiological stress markers in chickens

Cited by 46 publications

References 43 publications

Comparison of Stress Response in Diallel Crossed Korean Domestic Chicken Breeds

Comparison of Stress Response in Diallel Crossed Korean Domestic Chicken Breeds

The Relationship of the Expressions of Stress-related Markers and Their Production Performances in Korean Domestic Chicken Breed

Age-Dependent Variation of Telomere Length and DNA Damage in Chicken

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