Telomeres and seed banks

Boubriak, Ivan; Polischuk, V. P.; Grodzinsky, A. M.; Osborne, Daphne J.

doi:10.3103/s0095452707010045

Cited by 11 publications

(12 citation statements)

References 17 publications

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“…In plants, the association between telomere shortening and ageing is not as clear as in animals. A few studies, carried out in seeds, have, however, evidenced the correlation between telomere length and seed ageing (Boubriak et al, 2007). The relevance of the interaction between topo I and telomere DNA has been reported in yeast (Lotito et al, 2008); nevertheless, the possible effects of Tdp1 gene depletion on telomere homeostasis are still unknown, in animal as well in plant cells.…”

Section: -Test) (B)mentioning

confidence: 99%

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

Donà¹,

Confalonieri

Minio

et al. 2013

Journal of Experimental Botany

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An intron-spliced hairpin RNA approach was used for the targeted silencing of the MtTdp1α gene encoding the αisoform of tyrosyl-DNA phosphodiesterase 1 in Medicago truncatula Gaertn. Tyrosyl-DNA phosphodiesterase 1, involved in the repair of DNA topoisomerase I-mediated DNA damage, has been poorly investigated in plants. RNASeq analysis, carried out in the MtTdp1α-depleted plants, revealed different levels of transcriptional modulation (up-and down-regulation, alternative splicing, activation of alternative promoter) in genes involved in DNA damage sensing, DNA repair, and chromatin remodelling. It is suggested that the MtTdp1α gene has new, previously undetected roles in maintaining genome integrity. Up-regulation of senescence-associated genes and telomere shortening were observed. Moreover, impaired ribosome biogenesis indicated that the MtTdp1α gene is required for the nucleolar function. In agreement with the RNA-Seq data, transmission electron microscopy detected an altered nucleolar architecture in the MtTdp1α-depleted cells. Based on the reported data, a working hypothesis related to the occurrence of a nucleolar checkpoint in plant cells is proposed.

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Section: -Test) (B)mentioning

confidence: 99%

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

Donà¹,

Confalonieri

Minio

et al. 2013

Journal of Experimental Botany

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“…Extrachromosomal telomeric DNA was not genetically stable and rapidly disappeared after germination. Progressive excision of chromosomal telomeric sequences which correlated with decreased germination was also observed during storage of rye seeds [55] . As there is no proliferation activity in quiescent seeds, the loss of telomeric DNA must be replication independent and, thus, cannot simply be due to the end-replication problem.…”

Section: Telomeres and Seed Longevitymentioning

confidence: 96%

Telomeres, Aging, and Plants: From Weeds to Methuselah – A Mini-Review

Watson

Říha

2010

Gerontology

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The process of aging affects most, if not all, living creatures, from single celled yeast to multi-cellular mammals and plants. The DNA end-replication problem along with the tissue-limited expression of telomerase led to the telomere hypothesis of aging, where limits on cellular proliferation are genetically encoded in the lengths of a cell’s telomeres. Support for this hypothesis has been found in several organisms, from worms to mice to humans. While development, and therefore the process of aging, is quite different between plants and animals, telomere biology between these organisms is fundamentally the same. Do telomeres, then, also play the role of a molecular clock in plants? In this review, we explore the current knowledge of the relationship between telomeres and aging in plants in three specific cases: leaf senescence, aging of perennials and seed longevity.

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“…Despite having identical germination rates, significant differences in telomeric lengths were reported in both fresh and stored wheat seeds (Bucholc and Buchowicz, 1992;Bucholc and Buchowicz, 1995). Various studies have reported a strong association between aging and telomere length, as indicated by the breakdown of telomeric sequences (Boubriak et al, 2007). Dona et al (2013) also found that the telomeric length of seeds from two alpine species, Silene acaulis and Silene vulgaris, decreased and increased significantly upon drying and rehydration, respectively.…”

Section: Dna Methylationmentioning

confidence: 99%

“…Seed aging was assessed effectively by measuring the length of telomeres in seeds (Boubriak et al, 2007;Dona et al, 2013). Therefore, telomeric investigation has been proposed as a reliable marker of seed aging, and both Southern blotting and qRT-PCR have been used for telomere length analysis (Boubriak et al, 2007;Dona et al, 2013).…”

Section: Genomic Methylation Analysismentioning

confidence: 99%

Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

Ahmad¹,

Shah²,

Rehman³

et al. 2017

Current Issues in Molecular Biology

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Abbreviations:AFLP, amplified fragment length polymorphism AGO, argonaute miRNA, micro RNA NGS, next generation sequencing PCD, programmed cell death PIMT, protein L-isoaspartyl methyltransferase PRC2, Polycomb repressive complex 2 RAPD, random amplification of polymorphic DNA ROS, reactive oxygen species SSR, simple sequence repeat TUNEL, terminal deoxynucleotide transferasemediated dUTP nick-end labeling qRT-PCR, quantitative reverse transcriptional polymerase chain reaction Abstract For human food security, the preservation of 7.4 million ex-situ germplasm is a global priority. However, ex situ-conserved seeds are subject to aging, which reduces their viability and ultimately results in the loss of valuable genetic material over long periods. Recent progress in seed biology and genomics has revealed new opportunities to improve the long-term storage of ex-situ seed germplasm. This review summarizes the recent improvements in seed physiology and genomics, with the intention of developing genomic tools for evaluating seed aging. Several lines of seed biology research have shown promise in retrieving viability signal from various stages of seed germination. We conclude that seed aging is associated with mitochondrial alteration and programmed cell death, DNA and enzyme repair, anti-oxidative genes, telomere length, and epigenetic regulation. Clearly, opportunities exist for observing seed aging for developing genomic tools to increment the traditional germination test for effective conservation of ex-situ germplasm.

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Telomeres and seed banks

Cited by 11 publications

References 17 publications

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

RNA-Seq analysis discloses early senescence and nucleolar dysfunction triggered by Tdp1α depletion in Medicago truncatula

Telomeres, Aging, and Plants: From Weeds to Methuselah – A Mini-Review

Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

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