Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and <i>ex-situ</i> Conservation

Ahmad, Zaheer; Shah, Zahid Hussain; Rehman, Hafiz Mamoon; Shahzad, Khurram; Daur, İhsanullah; Elfeel, Abdalla; Hassan, Mahmood Ul; Elsafori, Ali Khalid; Yang, Seung Hwan; Chung, Gyuhwa

doi:10.21775/cimb.022.089

Cited by 8 publications

(7 citation statements)

References 113 publications

(163 reference statements)

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“…Oxidative stress is a pathological process manifesting overproduction of reactive oxygen species (ROS) and reactive nitrogen species (RNS) under harmful stimuli 74 - 79 . These alterations result in an imbalance of antioxidants and ROS/RNS and cause immense injury to neurons.…”

Section: Regulation Of Ampk In Ischemic Strokementioning

confidence: 99%

AMPK: Potential Therapeutic Target for Ischemic Stroke

et al. 2018

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5'-AMP-activated protein kinase (AMPK), a member of the serine/threonine (Ser/Thr) kinase group, is universally distributed in various cells and organs. It is a significant endogenous defensive molecule that responds to harmful stimuli, such as cerebral ischemia, cerebral hemorrhage, and, neurodegenerative diseases (NDD). Cerebral ischemia, which results from insufficient blood flow or the blockage of blood vessels, is a major cause of ischemic stroke. Ischemic stroke has received increased attention due to its '3H' effects, namely high mortality, high morbidity, and high disability. Numerous studies have revealed that activation of AMPK plays a protective role in the brain, whereas its action in ischemic stroke remains elusive and poorly understood. Based on existing evidence, we introduce the basic structure, upstream regulators, and biological roles of AMPK. Second, we analyze the relationship between AMPK and the neurovascular unit (NVU). Third, the actions of AMPK in different phases of ischemia and current therapeutic methods are discussed. Finally, we evaluate existing controversy and provide a detailed analysis, followed by ethical issues, potential directions, and further prospects of AMPK. The information complied here may aid in clinical and basic research of AMPK, which may be a potent drug candidate for ischemic stroke treatment in the future.

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Section: Regulation Of Ampk In Ischemic Strokementioning

confidence: 99%

AMPK: Potential Therapeutic Target for Ischemic Stroke

et al. 2018

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“…Both molecules have been shown to interact with proteins (leading to loss of function) and DNA (leading to mutations) or inhibit DNA and protein synthesis [ 53 , 56 ]. Besides, epigenetic regulation, in particular DNA methylation, has been proposed as a possible indicator of seed ageing [ 57 ] as it has been related to viability loss during seed storage as the following reported studies show.…”

Section: Seed Conservationmentioning

confidence: 99%

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

González‐Benito

Ibáñez

Pirredda

et al. 2020

IJMS

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Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

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“…[43][44][45] Therefore, it is necessary to study aging during CIK cell proliferation and self-renewal by monitoring the activity or magnitude of telomeres on day 0, 7, and day 14. [46][47][48][49] Although there is no significant evidence to suggest the correlation between the efficiency of tumor immunotherapy and the age of CIK cells, understanding of this correlation is important for the effective development of CIK cell-based immunotherapy.…”

Section: The Aging Of Cik Cellsmentioning

confidence: 99%

Cell-based immunotherapy with cytokine-induced killer (CIK) cells: From preparation and testing to clinical application

Meng

Zhong

et al. 2017

Human Vaccines & Immunotherapeutics

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Cell-based immunotherapy holds promise in the quest for the treatment of cancer, having potential synergy with surgery, chemotherapy and radiotherapy. As a novel approach for adoptive cell-based immunotherapy, cytokine-induced killer (CIK) cells have moved from the 'bench to bedside'. CIK cells are a heterogeneous subset of ex-vitro expanded, polyclonal T-effector cells with both natural killer (NK) and Tcell properties, which present potent non-major histocompatibility complex-restricted cytotoxicity against a variety of tumor target cells. Initial clinical studies on CIK cell therapy have provided encouraging results and revealed synergistic antitumor effects when combined with standard therapeutic procedures. At the same time, issues such as inadequate quality control and quantity of CIK cells as well as exaggerated propaganda were continuously emerging. Thus, the Ministry of Health in China stopped CIK cell therapy in May 2016, which was a major setback for the innovation of CIK cell-based immunotherapy. Thus, it is very important to modify technical criteria to develop a standardized operation procedure (SOP) and standardized system for evaluating antitumor efficacy in a safe way.

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Genomics: A Hallmark to Monitor Molecular and Biochemical Processes Leading Toward a Better Perceptive of Seed Aging and ex-situ Conservation

Cited by 8 publications

References 113 publications

AMPK: Potential Therapeutic Target for Ischemic Stroke

AMPK: Potential Therapeutic Target for Ischemic Stroke

Application of the MSAP Technique to Evaluate Epigenetic Changes in Plant Conservation

Cell-based immunotherapy with cytokine-induced killer (CIK) cells: From preparation and testing to clinical application

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