Telomerase Expression in Normal Epithelium, Reactive Atypia, Squamous Dysplasia, and Squamous Cell Carcinoma of the Uterine Cervix

Shroyer, Kenneth R.; Thompson, Lynn; Enomoto, Takayuki; ESKENS, JOAN L.; Shroyer, A. Laurie; McGregor, James A.

doi:10.1093/ajcp/109.2.153

Cited by 43 publications

(48 citation statements)

References 26 publications

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“…The cytology reports for these samples were inflammation (n ¼ 2) and ASC-US (n ¼ 1) and the colposcopic examination revealed cervical erosion in four cases. This is in agreement with earlier studies that showed presence of mild telomerase activity even in ASC-US, inflammation and cervical erosions (Kyo et al, 1997;Shroyer et al, 1998;Wisman et al, 2000). It is also known that the presence of HPV 16 E6 protein can activate telomerase activity irrespective of cells entering 'crisis' stage (Klingelhutz et al, 1996).…”

Section: Discussionsupporting

confidence: 92%

“…High telomerase activity in all samples of CIS, at par with invasive carcinomas, is justified since this stage is a pre-invasive stage. Similar results were reported by several other authors (Kyo et al, 1997;Zheng et al, 1997;Shroyer et al, 1998;Zhang et al, 1999;Wisman et al, 2000). Meeker and Coffey (1997) noted 46% telomerase positivity in cervical precancerous tissues where as Snijders et al (1998) reported low telomerase positivity in CIN lesions.…”

Section: Discussionsupporting

confidence: 85%

“…Reddy et al (2001) reported 100% telomerase positivity in 29 cases of cervical intraepithelial neoplasia (CIN) IB, IIB, IIIA and IIIB. Several authors (Kyo et al, 1997;Meeker and Coffey, 1997;Zheng et al, 1997;Shroyer et al, 1998;Wisman et al, 2000) in survey on human malignancies reported 88 -100% telomerase activity. Cervical cancers exhibit high telomerase activity irrespective of histopathology grading but the intensity/level of telomerase activity increased with the clinical progression of the disease.…”

Section: Discussionmentioning

confidence: 99%

“…The average telomerase activity increased with the progression of the clinical stage. The gradual elevation of telomerase activity from mild to moderate to high to very high telomerase activity in different dysplsia and invasive cancer samples indicate that with the progression of the disease there is a concomitant increase in the telomerase activity (Kyo et al, 1997;Shroyer et al, 1998: Wisman et al, 2001Baege et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

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Telomerase activity as an adjunct to high-risk human papillomavirus types 16 and 18 and cytology screening in cervical cancer

et al. 2006

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Telomerase is a ribonucleoprotein comprising an RNA template, the telomerase-associated protein and its catalytic subunit, human telomerase reverse transcriptase (hTERT). Telomerase activation is a critical step in cellular immortalisation and development of cancer. Enhanced telomerase activity has been demonstrated in cervical cancer. In the present study telomerase activity and hTERT mRNA expression were evaluated and correlated with the presence of human papillomavirus (HPV) infection and cytological changes in the cervical lesions. Telomerase activity was assayed by telomeric repeat amplification protocol, hTERT mRNA expression by reverse transcriptase polymerase chain reaction and presence of high risk HPV (HR-HPV) infection by polymerase chain reaction. Out of 154 cervical samples of different cytology, 90 (58.44%) were positive for HR-HPV types 16/18, while among 55 normal cervical scrapes, 10 (18.18%) were HPV DNA positive. All 59 invasive cancer samples showed a very high telomerase activity. Among dysplasia, seven (63.6%) mild dysplasia, 18 (100%) of moderate, 20 (100%) of severe dysplasia and 6 (100%) carcinoma in situ (CIS) samples were positive with mild to moderate to high to very high telomerase activity respectively. Seven (12.7%) samples of apparently normal cervical scrapes were weakly positive for telomerase activity. We observed a good correlation (Po0.001) between telomerase activity and HR-HPV 16/18 positivity with a sensitivity of 88.1% for HPV and 100% for telomerase activity. It is suggested that telomerase activity may be used as an adjunct to cytology and HPV DNA testing in triaging women with cervical lesions.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Telomerase activity as an adjunct to high-risk human papillomavirus types 16 and 18 and cytology screening in cervical cancer

et al. 2006

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“…17,24,25 Telomerase is a ribonucleoprotein DNA polymerase containing an RNA component, designated hTR, and a single catalytic protein subunit, human telomerase reverse transcriptase (hTERT), which directs the synthesis of telomeric DNA repeats. Telomerase contributes to cellular immortalization as a fundamental component of the processes of malignant transformation and may play a central role in HPV-mediated transformation of the cervical mucosa.…”

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confidence: 99%