Innovations in understanding the biology of cervical cancer

Wolf, Judith K.; Ph.D., M.P.H. Eduardo L. Franco; Arbeit, Jeffery M.; Shroyer, Kenneth R.; Wu, T. C.; Runowicz, Carolyn D.; Tortolero‐Luna, Guillermo; Herrero, Rolando; Crum, Christopher P.

doi:10.1002/cncr.11682

Cited by 32 publications

(25 citation statements)

References 32 publications

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“…The importance of oncogenes and tumor suppressor genes and their protein products as they pertain to cervical carcinogenesis must be considered as well [80]. The epidermal growth factor (EGFR) receptor is a 170-kD transmembrane glycoprotein that, when activated, promotes cell growth in a variety of normal and transformed tissues [81].…”

Section: Looking Ahead Beyond 204mentioning

confidence: 99%

Gynecologic oncology group trials of chemotherapy for metastatic and recurrent cervical cancer

Tewari

Monk

2005

Curr Oncol Rep

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Section: Looking Ahead Beyond 204mentioning

confidence: 99%

Gynecologic oncology group trials of chemotherapy for metastatic and recurrent cervical cancer

Tewari

Monk

2005

Curr Oncol Rep

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“…Those that are found in cervical and oral cancers are often referred as "mucosal high risk" type, among which HPV-16 is probably the most carcinogenic. 2 The oncogenic property of HPV has been assigned primarily to two early genes, E6 and E7, which are expressed in HPV-containing cells and carcinomas, and act as transforming genes both in vitro and in vivo. 3 Evidence has suggested that expression of the HPV E6 and E7 genes is necessary for continued growth of most cervical cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Ribozyme targeting HPV16 E6E7 transcripts in cervical cancer cells suppresses cell growth and sensitizes cells to chemotherapy and radiotherapy

Zheng

Zhang²,

Rao³

2004

Cancer Biology & Therapy

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ACKNOWLEDGEMENTSWe thank Lianghu Qu for his help in experiment design and Yuntao Wu for critical readings of the manuscript. ABSTRACTHuman Papillomavirus (HPV) is related to more than 90% of cervical cancer. The virus is shown to be essential for the induction and maintenance of the malignant phenotype in cervical cancer. In this report, we designed a hammerhead ribozyme Rz170 to specifically target the HPV16 E6E7 transcripts, and our results demonstrated that Rz170 can cleave HPV16 E6E7 transcripts effectively and with high specificity. When transfected into a HPV16 positive cervical cancer cell, CaSKi, the ribozyme reduced the expression of HPV16 E6 and E7 mRNA, and inhibited cell growth both in vitro and in vivo. The percentage of apoptosis cells was also increased. We found that Rz170 reduced the expression of the viral E6 and E7 proteins, and cellular c-myc, bcl-2 proteins, but increased the expression of p53 and Rb proteins. It is likely that the ribozyme inhibited cervical cancer cell growth by reducing the expression of the HPV16 E6 and E7gene, which may alter the expression of p53, Rb, c-myc and bcl-2, and led to apoptosis in cancer cells. We also found that CaSKi cells transfected with Rz170 showed increased sensitivity to cisplatin and radiation. Our study demonstrated the potential of Rz170 for treating cervical cancer, and the possibility of using a combined therapeutic strategy involving ribozyme, chemotherapy or radiotherapy.

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“…Several risk factors have been identified for the occurrence of cervical cancer such as the human papilloma virus (HPV), which is responsible for more than 90% of the cases of invasive cervical cancer worldwide [5][6][7][8][9], and being a sexually active female [10].…”

Section: Introductionmentioning

confidence: 99%

Practice and Barriers towards Cervical Cancer Screening among University Staff at a Malaysian University

Naggar¹

2012

J Community Med Health Edu

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Objective: The objective of this study was to determine the practice and barriers towards the Pap smear test among Malaysian women.Methodology: This is a cross-sectional survey of female university staff. Data was collected by a self-administered questionnaire from a total of 117 women. T-test and ANOVA test were conducted to determine if there was a significant difference between the study parameters.Results: A total number of 117 female university staff participated in this study. The majority of them were Malays, single, and living in the city (92.3%, 58.1%, and 92.3%; respectively). Regarding lifestyle practices, the majority consumed vitamin and mineral supplements regularly, and exercised once a week, (55.6%, and 41%, respectively). As for their knowledge about cervical cancer, the majority have heard about the Pap smear test before (81.2%). Regarding the practice of Pap smear test, only 22.2% ever had a Pap smear test done. As for the barriers toward having a Pap smear test done, the most common barrier among study participants was lack of time (29.9%), followed by the excuse that the Pap smear test is a painful procedure (17.9%). Regarding the factors that influenced the practice of having a Pap smear test were marital status, occupation, regular vitamin and mineral supplements intake, daily fruits intake, regular medical check-up, educational and income level significantly influenced the practice (p=0.001, p=0.002, p=0.034, p=0.001, p=0.001, p=0.024, p=0.001; respectively).

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Innovations in understanding the biology of cervical cancer

Abstract: Revelation of the connection between the human papillomavirus (HPV) and cervical neoplasia and invasive cervical cancer is prompting new investigations to expand that understanding and promote vaccines, gene therapy, and other inter-

Cited by 32 publications

References 32 publications

Gynecologic oncology group trials of chemotherapy for metastatic and recurrent cervical cancer

Gynecologic oncology group trials of chemotherapy for metastatic and recurrent cervical cancer

Ribozyme targeting HPV16 E6E7 transcripts in cervical cancer cells suppresses cell growth and sensitizes cells to chemotherapy and radiotherapy

Practice and Barriers towards Cervical Cancer Screening among University Staff at a Malaysian University

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