Telomerase Activity Is Decreased in Peripheral Blood Mononuclear Cells of Hemodialysis Patients

Tsirpanlis, George; Chatzipanagiotou, Stylianos; Boufidou, Fotini; Kordinas, Vasileios; Alevyzaki, Fotini; Zoga, Margarita; Kyritsis, Ilias; Stamatelou, Kyriaki; Triantafyllis, George; Nicolaou, Chryssoula

doi:10.1159/000092031

Cited by 43 publications

(34 citation statements)

References 33 publications

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“…Here we demonstrated that both telomerase activity and expression are affected in patients with a decline in renal function, a fact that might be associated with the increased inflammation markers. These conclusions concur with the results of two previous studies conducted in our laboratory [34,35]. In addition one cannot overlook the negative statistical relationship between the rise in IL-6 serum concentration and the decrease in telomerase activity.…”

Section: Discussionsupporting

confidence: 92%

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Kordinas

Tsirpanlis

Nicolaou

et al. 2015

Cellular and Molecular Biology Letters

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Telomerase is involved in the elongation of telomeres. It remains active in very few types of cell in mature organisms. One such cell type is the lymphocytes. In this study, we investigated the activity and expression of telomerase in lymphocytes from renal failure patients and compared it to that for normal controls. Inflammation status was determined at the same time. The enzyme activity was measured using PCR-ELISA with peripheral blood mononuclear cells (PBMCs) from three groups: 53 healthy individuals, 50 patients with chronic kidney disease (CKD) and 50 dialysis patients. In the same cell populations, the expression of the reverse transcriptase of the human telomerase gene (hTERT) was measured via real-time PCR. The inflammationstatus of these individuals was determined by calculating the interleukin 6 (IL-6), IL-10, C-reactive protein (CRP) and tumor necrosis factor alpha (TNF-a) serum concentrations via ELISA. The lowest levels of telomerase activity were detected in CKD, and this group had the highest IL-6 and CRP values and the lowest hTERT expression. The dialysis group showed significant differences in comparison to the normal subjects and to the CKD patients. Further studies are warranted in order to explore the way inflammation influences telomerase activity and hTERT expression.

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Section: Discussionsupporting

confidence: 92%

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Kordinas

Tsirpanlis

Nicolaou

et al. 2015

Cellular and Molecular Biology Letters

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“…sCD163 was determined using an ELISA kit from Trillium Diagnostics as previously described [12]. The method for detection of telomerase activity and isolation of PBMC is described elsewhere [14]. Briefly, telomerase activity in PBMC was measured using a commercial telomerase PCR-ELISA (Roche Diagnostics Corp., Indianapolis, IN, USA), based on the telomeric repeat amplification protocol [15].…”

Section: Methodsmentioning

confidence: 99%

Connection between Telomerase Activity in PBMC and Markers of Inflammation and Endothelial Dysfunction in Patients with Metabolic Syndrome

et al. 2012

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Metabolic syndrome (MS) is a constellation of metabolic derangements associated with vascular endothelial dysfunction and oxidative stress and is widely regarded as an inflammatory condition, accompanied by an increased risk for cardiovascular disease. The present study tried to investigate the implications of telomerase activity with inflammation and impaired endothelial function in patients with metabolic syndrome. Telomerase activity in circulating peripheral blood mononuclear cells (PBMC), TNF-α, IL-6 and ADMA were monitored in 39 patients with MS and 20 age and sex-matched healthy volunteers. Telomerase activity in PBMC, TNF-α, IL-6 and ADMA were all significantly elevated in patients with MS compared to healthy volunteers. PBMC telomerase was negatively correlated with HDL and positively correlated with ADMA, while no association between TNF-α and IL-6 was observed. IL-6 was increasing with increasing systolic pressure both in the patients with MS and in the healthy volunteers, while smoking and diabetes were positively correlated with IL-6 only in the patients' group. In conclusion, in patients with MS characterised by a strong dyslipidemic profile and low diabetes prevalence, significant telomerase activity was detected in circulating PBMC, along with elevated markers of inflammation and endothelial dysfunction. These findings suggest a prolonged activity of inflammatory cells in the studied state of this metabolic disorder that could represent a contributory pathway in the pathogenesis of atherosclerosis.

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“…It was suggested that these resulted from chronic activation due to major histocompatibility complex incompatibility and immunosuppressive therapy. Tsirpanlis et al [77] measured the activity of telomerase (the enzyme that preserves telomere length and structure and thus prevents senescence [78] ) in peripheral blood mononuclear cells in hemodialysisdependent patients and nonrenal failure subjects. Telomerase activity was reduced in hemodialysis patients compared to healthy controls, and was lower in longterm than in short-term dialysis patients.…”

Section: Autophagy In Uremiamentioning

confidence: 99%

Aging and uremia: Is there cellular and molecular crossover?

White

Yaqoob

Harwood

2015

WJN

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immune systems. However, whilst much has been documented about the shared physical characteristics of aging and uremia, the molecular and cellular similarities between the two have received less attention. In order to bridge this perceived gap we have reviewed published research concerning the common molecular processes seen in aging subjects and CKD patients, with specific attention to altered proteostasis, mitochondrial dysfunction, post-translational protein modification, and senescence and telomere attrition. We have also sought to illustrate how the cell death and survival pathways apoptosis, necroptosis and autophagy are closely interrelated, and how an understanding of these overlapping pathways is helpful in order to appreciate the shared molecular basis behind the pathophysiology of aging and uremia. This analysis revealed many common molecular characteristics and showed similar patterns of cellular dysfunction. We conclude that the accelerated aging seen in patients with CKD is underpinned at the molecular level, and that a greater understanding of these molecular processes might eventually lead to new much needed therapeutic strategies of benefit to patients with renal disease. AbstractMany observers have noted that the morphological changes that occur in chronic kidney disease (CKD) patients resemble those seen in the geriatric population, with strikingly similar morbidity and mortality profiles and rates of frailty in the two groups, and shared characteristics at a pathophysiological level especially in respect to the changes seen in their vascular and REVIEW

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Telomerase Activity Is Decreased in Peripheral Blood Mononuclear Cells of Hemodialysis Patients

Cited by 43 publications

References 33 publications

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?

Connection between Telomerase Activity in PBMC and Markers of Inflammation and Endothelial Dysfunction in Patients with Metabolic Syndrome

Aging and uremia: Is there cellular and molecular crossover?

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